Physiological Effect of Ataulfo Mango (Manguifera Indica) Beverage in Exercise

Exercise-induced Oxidative Stress Clinical Trial

Official title:

Physiological Response to Ataulfo Mango Pulp and Peel Beverages in Exercise

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03182543
• Other study ID #: UACJ-ICB-2017-01
• Status: Completed
• Phase: N/A
• First received: May 29, 2017
• Last updated: June 7, 2017
• Start date: January 1, 2017
• Est. completion date: April 30, 2017

Study information

• Verified date: June 2017
• Source: Universidad Autonoma de Ciudad Juarez
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The aim of this study is to assess the antioxidant capacity of two mango base beverages (AM1, AM2), against exercise-induced oxidative stress.

Description:

Introduction. The post-consumption physiological effect of mango cv. "Ataulfo" pulp (MP) and peel (MC) in humans before exercise has been poorly evaluated.

Objective. To evaluate the physiological response, to consumption of two mango base beverages, AM1 (200g MP/ 600mL water) and AM2 (160g MP; 40g MC / 600mL water), before and after a submaximal exercise test.

Methods. The antioxidant profile and physicochemical characteristics of AM1 and AM2 were evaluated. Several cardiorespiratory, anthropometric and body composition parameters of 19 university students, prior to the consumption of AM1, AM2 and water, were evaluated in three non-consecutive sessions (self-control cases). The glycemic, lactic and antioxidant (FRAP), uric acid (AU), reduced glutathione (GSH) and lipid oxidation (TBARS) and protein carbonyls (PC) responses were evaluated in plasma before and after an incremental cycle ergometer exercise test.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 19
• Est. completion date: April 30, 2017
• Est. primary completion date: February 28, 2017
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 17 Years to 28 Years

Eligibility

Inclusion Criteria:

• healthy male

• university student

• no alcohol, cigar or drugs consumption

Exclusion Criteria:

• no antioxidant supplement or ergogenic consumption

• health issues with performing physical activity

Related Conditions & MeSH terms

• Exercise-induced Oxidative Stress

Intervention

Dietary Supplement:
• Mango pulp beverage
The subject consumes mango pulp beverage (200 g mango pulp + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.
• Mango pulp and peel beverage
The subject consumes mango pulp and peel beverage (160 g mango pulp + 40 g mango peel + 600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.
• Control beverage
The subject consumes control beverage (600 mL water). 2 hours later subject performs the exercise test. Washout: 1 week washout period prior to the administration of this intervention to avoid possible carryover effect.

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Universidad Autonoma de Ciudad Juarez

References & Publications (5)

Alvarez-Parrilla E, De La Rosa LA, Legarreta P, Saenz L, Rodrigo-García J, González-Aguilar GA. Daily consumption of apple, pear and orange juice differently affects plasma lipids and antioxidant capacity of smoking and non-smoking adults. Int J Food Sci Nutr. 2010 Jun;61(4):369-80. doi: 10.3109/09637480903514041. — View Citation

Ramos-Jiménez A, Hernández-Torres RP, Wall-Medrano A, Torres-Durán PV, Juárez-Oropeza MA, Solis Ceballos JA. Acute physiological response to indoor cycling with and without hydration; case and self-control study. Nutr Hosp. 2013 Sep-Oct;28(5):1487-93. doi: 10.3305/nh.2013.28.5.6698. — View Citation

Spirlandeli AL, Deminice R, Jordao AA. Plasma malondialdehyde as biomarker of lipid peroxidation: effects of acute exercise. Int J Sports Med. 2014 Jan;35(1):14-8. doi: 10.1055/s-0033-1345132. Epub 2013 Jun 14. — View Citation

Wall-Medrano A, Olivas-Aguirre FJ, Velderrain-Rodriguez GR, González-Aguilar A, de la Rosa LA, López-Díaz JA, Álvarez-Parrilla E. [Mango: agroindustrial aspects, nutritional/functional value and health effects]. Nutr Hosp. 2014 Nov 1;31(1):67-75. doi: 10.3305/nh.2015.31.1.7701. Review. Spanish. — View Citation

Wang CY, Haskell WL, Farrell SW, Lamonte MJ, Blair SN, Curtin LR, Hughes JP, Burt VL. Cardiorespiratory fitness levels among US adults 20-49 years of age: findings from the 1999-2004 National Health and Nutrition Examination Survey. Am J Epidemiol. 2010 Feb 15;171(4):426-35. doi: 10.1093/aje/kwp412. Epub 2010 Jan 15. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Antioxidant capacity change	Assessed by FRAP change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 min (immediately after exercise test)
Primary	Antioxidant capacity change	Assessed by uric acid concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 (immediately after exercise test)
Primary	Antioxidant capacity change	Assessed by reduced glutathione concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 (immediately after exercise test)
Secondary	Oxidative stress change	Assessed by TBARS concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 (immediately after exercise test)
Secondary	Oxidative stress change	Assessed by protein carbonyls concentration change. Blood samples were drawn from the antecubital vein into EDTA test tubes at rest, before beverage intake, and immediately following the exercise test. Samples were then centrifuged at 5000 rpm for 10 min at 4°C to obtain plasma, which was withdrawn and separated into eppendorf vials, maintained at -80°C until further biochemical analysis.	0 min (before beverage intake), 9-15 (immediately after exercise test)
Secondary	Lactic acid concentration change	Assessed by lactic acid concentration change. Capillary blood samples were taken from the fingertip, using a lancet and analyzed (YSI modelo 1500, Yellow Springs, USA)	0 min (before performing exercise test), 3 min, 6 min, 9 min, 12 min, 15 min (during exercise test)
Secondary	Postprandial glycemic change	Assessed by blood glucose concentration change. Capillary blood samples were taken from the fingertip, using a lancet, and analyzed (ReliOn Confirm/micro Test Strips ARKRAY, USA)	0 min (before beverage intake), 30 min, 60 min, 90 min, 120 min (after beverage intake)