Sporadic Amyotrophic Lateral Sclerosis Clinical Trial
Official title:
Bioenergetics and Protein Metabolism in Sporadic Amyotrophic Lateral Sclerosis
INTRODUCTION: Amyotrophic Lateral Sclerosis (ALS) is a degenerative disease of the motor
neurones of the brain and the spinal cord. The pathophysiological mechanisms of the disease
remain unknown. The average age of onset of ALS is about 60 years old, and the mean survival
of patients is about 2 years. The disease is responsible for a progressive paralysis leading
to death from respiratory failure. The only treatment available is the Riluzole, with a very
modest efficiency on the progression of the disease. ALS is the third neurodegenerative
disease, affecting 6000 persons in France, 150 000 in the world. Among the protagonists
involved in the occurrence of the disease, mitochondrial perturbations and protein
accumulations seem to be central elements.
OBJECTIVES: To precise the implication of energy and protein metabolism in the sporadic
forms of ALS, to identify potential biomarkers of the disease and to test new therapies.
METHODS: The investigators will study cell growth, bioenergetics, mitochondrial dynamics,
free-radicals production, presence of cytoplasmic inclusions, cytoskeleton structure and
stress response in primary skin fibroblasts obtained from sporadic ALS patients. The study
will be conducted over a period of three years in 3 centres specialized respectively in
motor neuron diseases, mitochondrial metabolism and neuronal cytoskeleton.
PROSPECTS: If the investigators achieve to identify differences between ALS fibroblasts and
controls, the results will be key elements to reinforce the hypothesis of a systemic disease
with an important metabolic participation, to better define ALS pathophysiological
mechanisms, to find potential biomarkers and to test new therapies.
The investigators will study fibroblasts derived from sporadic ALS patients.
Clinical parameters will be collected such as sex, age, age of onset, clinical presentation,
electrophysiological an respiratory parameters, treatments.
Patients and controls derived fibroblasts will be amplified and cultivated from skin
biopsies.
Primary fibroblast cell cultures will be derived from skin biopsies obtained from control
subjects and from the patients after obtaining written consent. Amplifications will be
performed, from passage 1 to 6, allowing the time for cell cryopreservation of 10 samples
per cell line. All experiments will be conducted on cells with similar passage numbers,
ranging from 6 to 25, to avoid artefacts due to senescence.
Cell growth of each line will be monitored with the IncuCyte ZOOM® system (Essen
Biosciences), a microscope gantry that resides in a cell incubator, and a networked external
controller hard drive that gathers and processes image data. Cell growth will be
automatically calculated at each time point using the relative cell density (ratio of the
occupied area to the total area of the acquired regions).
The respiratory parameters of the fibroblasts will be monitored precisely with an high
resolution oxygraph (OROBOROS). Enzymatic activity of each complex of the mitochondrial
respiratory chain will be measured by spectrometry, and the mitochondrial membrane potential
will be studied with fluorescence microscopy (TMRM probe). The investigators will also study
mitochondrial dynamics and the architecture of mitochondrial network with fluorescence
microscopy and super-resolution microscopy (SRM, Roper). The production of free radicals
will be estimated with probes sensitive to oxidative stress. Finally, a metabolomic study
(mass spectrometry QTRAP LC/MS/MS 5500 ABSciex) will be performed to characterise precisely
the metabolites in the cells and to assess their metabolic profile.
The investigators will search for protein inclusions (intermediate filaments, TDP43, FUS,
ubiquitin) with immuno-fluorescence technics. Western Blots will be performed to study the
protein metabolism. The architecture of the cytoskeleton will be analysed in SRM and
fluorescence microscopy, and the investigators will try to transfect fibroblasts in order to
express Neurofilaments and study the consequences on the cytoskeleton and protein
metabolism.
The investigators will study the response of the cells to the stress (hypoxia SCI-tive Dual,
starvation), and to senescence (high passage numbers).
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Allocation: Non-Randomized, Intervention Model: Parallel Assignment, Masking: Open Label, Primary Purpose: Basic Science
Status | Clinical Trial | Phase | |
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Completed |
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