Familial Lipoprotein Lipase Deficiency Clinical Trial
Official title:
A Study to Determine the Safety and Efficacy in Lipoprotein Lipase-Deficient Subjects After Intramuscular Administration of AMT-011, an Adeno-Associated Viral Vector Expressing Human Lipoprotein LipaseS447X
Verified date | April 2010 |
Source | Amsterdam Molecular Therapeutics |
Contact | n/a |
Is FDA regulated | No |
Health authority | Canada: Health Canada |
Study type | Interventional |
LPLD is a rare autosomal recessive disorder, characterized by the presence of marked
chylomicronemia and hence hypertriglyceridemia. Clinically the most severe manifestation of
chylomicronemia, is acute pancreatitis, which can be lethal. There is no effective therapy
available to modulate the course of the illness and prevent complications for these
patients. The current clinical management consists of severe reduction of dietary fat that
is hard if not almost impossible to comply with. LPLD subjects continue to experience
pancreatitis attacks, and are admitted to intensive care units on several occasions.
Alipogene tiparvovec corrects or restores lipoprotein lipase (LPL) function long term, and
hence reverses some symptoms, halts the disease progression and prevents further
complications. Alipogene tiparvovec gene therapy ensures that a catabolically beneficial
variant of the human LPL gene, LPL[S447X] is expressed and active in the relevant tissues in
humans. Delivery of the gene is realized via intramuscular injection of an adeno-associated
viral vector, pseudotyped with AAV1 capsids.
Status | Active, not recruiting |
Enrollment | 14 |
Est. completion date | June 2013 |
Est. primary completion date | June 2013 |
Accepts healthy volunteers | No |
Gender | Both |
Age group | 18 Years and older |
Eligibility |
Inclusion Criteria: - Eligible Population Study participants must have participated in the preceding observation study (Prep-02: Appendix III) and be diagnosed with lipoprotein lipase deficiency, meeting the following criteria: (I) Their lipoprotein lipase activity levels in post-heparin plasma should be =20 % of normal; (II) Confirmed homozygocity or compound heterozygocity for mutations in the LPL gene; (III) Post-heparin plasma LPL mass should be >5% of normal; (IV) Median fasting plasma TG concentrations >10.00mmol/L, as determined on the basis of 5 consecutive time points in the preceding observation study with a history of pancreatitis. - General Health The participant must be in good general physical health with, in the opinion of the investigator, no other clinically significant and relevant abnormalities of medical history, and no abnormalities at the physical examination and routine laboratory evaluation performed prior to the trial. - Age Age =18 years old. - Sex Male or female. Females must be of non-child bearing potential or with a negative pregnancy test and not breast feeding. Female subjects must use appropriate contraception (if relevant) and their spouse must use barrier contraception for the duration of the study (12 weeks). Males must practice barrier birth control and their spouse should use appropriate contraception until three consecutive semen samples, taken at least 75 days after administration, are negative for AMT-011 vector DNA. - Compliance The participant is willing to fully comply with all study procedures and requirements of the trial such as restrictions to a low-fat diet (see section 8.1). - Consent The participant has the mental ability to give voluntary written informed consent to participate in the study. Exclusion Criteria: - Disease - Apolipoprotein CII deficiency. - Inflammatory muscle disease (e.g. myositis, myopathies or rhabdomolysis). - Any current or relevant previous history of serious, severe or unstable physical or psychiatric illness, any medical disorder that may make the participant unlikely to fully complete the study, or any condition that presents undue risk from the study medication or procedures (e.g. malignant neoplasia). - Active infectious disease of any nature, including clinically active viral infections. - Laboratory Parameters The following blood screening tests will result in exclusion from participation: - Platelet count < 100 x 109 /L. - Hemoglobin < 7.0 mmol/L. - Liver function disturbances (bilirubin >2.50 x normal, transaminases >3 x ULN). - CPK > 3 x ULN. - Creatinine > 3 x ULN. |
Allocation: Randomized, Endpoint Classification: Safety/Efficacy Study, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Treatment
Country | Name | City | State |
---|---|---|---|
Canada | Ecogene-21 Clinical Trial Center/ Centre de santé et de services sociaux de Chicoutimi | Chicoutimi | Quebec |
Lead Sponsor | Collaborator |
---|---|
Amsterdam Molecular Therapeutics | International Antiviral Therapy Evaluation Center |
Canada,
Burnett JR, Hooper AJ. Alipogene tiparvovec, an adeno-associated virus encoding the Ser(447)X variant of the human lipoprotein lipase gene for the treatment of patients with lipoprotein lipase deficiency. Curr Opin Mol Ther. 2009 Dec;11(6):681-91. — View Citation
Rip J, Nierman MC, Sierts JA, Petersen W, Van den Oever K, Van Raalte D, Ross CJ, Hayden MR, Bakker AC, Dijkhuizen P, Hermens WT, Twisk J, Stroes E, Kastelein JJ, Kuivenhoven JA, Meulenberg JM. Gene therapy for lipoprotein lipase deficiency: working toward clinical application. Hum Gene Ther. 2005 Nov;16(11):1276-86. — View Citation
Ross CJ, Liu G, Kuivenhoven JA, Twisk J, Rip J, van Dop W, Excoffon KJ, Lewis SM, Kastelein JJ, Hayden MR. Complete rescue of lipoprotein lipase-deficient mice by somatic gene transfer of the naturally occurring LPLS447X beneficial mutation. Arterioscler Thromb Vasc Biol. 2005 Oct;25(10):2143-50. Epub 2005 Jul 7. — View Citation
Ross CJ, Twisk J, Bakker AC, Miao F, Verbart D, Rip J, Godbey T, Dijkhuizen P, Hermens WT, Kastelein JJ, Kuivenhoven JA, Meulenberg JM, Hayden MR. Correction of feline lipoprotein lipase deficiency with adeno-associated virus serotype 1-mediated gene transfer of the lipoprotein lipase S447X beneficial mutation. Hum Gene Ther. 2006 May;17(5):487-99. — View Citation
Ross CJ, Twisk J, Meulenberg JM, Liu G, van den Oever K, Moraal E, Hermens WT, Rip J, Kastelein JJ, Kuivenhoven JA, Hayden MR. Long-term correction of murine lipoprotein lipase deficiency with AAV1-mediated gene transfer of the naturally occurring LPL(S447X) beneficial mutation. Hum Gene Ther. 2004 Sep;15(9):906-19. — View Citation
Stroes ES, Nierman MC, Meulenberg JJ, Franssen R, Twisk J, Henny CP, Maas MM, Zwinderman AH, Ross C, Aronica E, High KA, Levi MM, Hayden MR, Kastelein JJ, Kuivenhoven JA. Intramuscular administration of AAV1-lipoprotein lipase S447X lowers triglycerides in lipoprotein lipase-deficient patients. Arterioscler Thromb Vasc Biol. 2008 Dec;28(12):2303-4. doi: 10.1161/ATVBAHA.108.175620. Epub 2008 Sep 18. — View Citation
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Primary | Reduction of fasting triglyceride (TG) concentrations | To achieve a reduction in fasting plasma TG such that the difference in median plasma TG observed before administration and up to 12 weeks after administration represents approximately 40% reduction, on top of a low-fat diet. | 12 weeks | No |
Primary | safety profile of AMT-011 | Between 6 and 15 years after administration of AMT-011, patients will be annually contacted by phone to monitor delayed adverse events related to administration of AMT-011. | 15 years | Yes |
Secondary | Reduction of TG concentrations | To achieve sustained efficacy, defined as approximately 40% reduction in fasting plasma TG up to 26 weeks after administration, on top of a low-fat diet. | 26 weeks | No |
Secondary | Reduction of TG concentrations | To achieve a reduction in fasting plasma TG to a level equal to or less than 10.00 mmol/L on top of a low-fat diet at 12 weeks after administration. | 12 weeks | No |
Secondary | Reduction of TG concentrations | To achieve sustained efficacy, defined as a reduction in fasting plasma TG at 26 weeks after administration to a level equal to or less than 10.00 mmol/L on top of a low-fat diet. | 26 weeks | No |
Secondary | Biological activity and expression of the transgene product. | To determine the biological activity and expression of the lipoprotein lipase (LPLS447X) transgene product. | 1 year | No |
Secondary | Evaluation immune respons | To evaluate potential immune responses against the lipoprotein lipase (LPLS447X) transgene product and the adeno-associated viral (AAV) vector. | 5 years | Yes |
Secondary | To assess the shedding of the viral vector | To assess shedding of AMT-011 | 1 year | Yes |
Status | Clinical Trial | Phase | |
---|---|---|---|
Completed |
NCT00891306 -
Efficacy and Safety of Human Lipoprotein Lipase (LPL)[S447X] Expressed by an Adeno-Associated Viral Vector in LPL-deficient Subjects
|
Phase 2/Phase 3 |