Coffee Roasting and Glucose Tolerance

Oral Glucose Tolerance Clinical Trial

Official title:

Acute Effects of Light and Dark Roasted Coffee on Glucose Tolerance: A Randomized, Controlled Crossover Trial in Healthy Volunteers

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02417519
• Other study ID #: M217
• Status: Completed
• Phase: N/A
• First received: April 11, 2015
• Last updated: December 11, 2017
• Start date: April 2014
• Est. completion date: November 2015

Study information

• Verified date: December 2017
• Source: University of Copenhagen
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Chlorogenic acid (CGA) in coffee may lower the postprandial glucose response. CGA is destroyed by dark roasting. In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light (LIR) or dark (DAR) roasted coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Plasma glukose and insulin, appetite, and plasma and urine metabolic profiles will be analysed. The primary aim is to investigate whether roasting affects the postprandial glucose area under the curve (AUC).

Description:

Epidemiologic evidence suggests that coffee consumption is associated with a lower risk of type 2 diabetes. Coffee contains caffeine and several other components that may modulate glucose regulation. The chlorogenic acids (CGA) in coffee have been indicated as constituents that may help to normalize the acute glucose response after a carbohydrate challenge. The aim of this study was to investigate whether two coffee beverages that differ in CGA content due to different roasting degrees will affect glucose regulation differently.

In a controlled crossover trial, 11 healthy fasted volunteers consumed 300 mL of either light roasted (LIR) or dark roasted (DAR) coffee, or water, followed 30 min later by a 75-g oral glucose tolerance test (OGTT). Blood samples were drawn at baseline, 30, 60 and 120 min. Differences in glucose and insulin responses and insulin sensitivity index (ISI) were analyzed. The CGA and caffeine contents in the coffees were analyzed using UPLC-MS/MS.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 11
• Est. completion date: November 2015
• Est. primary completion date: September 2014
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 65 Years

Eligibility

Inclusion Criteria:

Body mass index (BMI) of 18.0-30.0 kg/m2 and with no known diseases

Exclusion Criteria:

• systemic infections,

• psychiatric or metabolic disorders,

• known food allergies or intolerances related to the products used in the study (e.g. dairy or gluten),

• ongoing or former drug abuse,

• high intake of alcohol (defined as a weekly intake of >7 units for women and > 14 units for men),

• pregnancy or ongoing planning of pregnancy,

• vegetarianism or veganism,

• participation in other scientific studies during the study period, and

• blood donation during - or in the month leading up to - the study period

Related Conditions & MeSH terms

• Oral Glucose Tolerance

Intervention

Other:
• DAR
Volunteers ingested 300 mL of coffee with a dark roast containing low levels of chlorogenic acids followed 30 min later by by 75g glucose and 300 mL water
• LIR
Volunteers ingested 300 mL of coffee with a light roast containing high levels of chlorogenic acids followed 30 min later by by 75g glucose and 300 mL water
• CTR
Volunteers ingested 300 mL of water containing no chlorogenic acids followed 30 min later by by 75g glucose and 300 mL water

Locations

Country	Name	City	State
Denmark	Department of Nutrition, Exercise and Sports, University of Copenhagen	Frederiksberg

Sponsors (1)

Lead Sponsor	Collaborator
University of Copenhagen

Country where clinical trial is conducted

Denmark, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Plasma glucose Area Under the Curve (Glucose AUC)	The area under the plasma glucose concentration curve was calculated from 0-120min after 75g glucose was ingested.	0-120 min
Secondary	Plasma glucose concentrations	changes in the glucose concentration determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate.	30, 60 and120 min
Secondary	Serum Insulin Area Under the Curve	Area under the curve (AUC) for serum insulin	0-120 min
Secondary	Serum insulin concentrations	changes in the insulin concentration determined by a mixed model analysis and subsequently at each time point with time 0 as a co-variate.	30, 60 and120 min
Secondary	ISI(0-120)	Matsuda's Insulin sensitivity index	0-120min
Secondary	Metabolic profile in plasma	Metabolic profile of plasma measured in all samples collected before the meal and postprandially from 0-120 min as well as in samples collected up to 24 hrs later.	0-24 hours
Secondary	Metabolic profile in urine	Metabolic profile of urine samples measured in all samples collected before the meal and postprandially from 0-120 min 120-240min as well as in samples collected up to 24 hrs later.	0-24 hours