Mechanical Ventilation Complication Clinical Trial
Official title:
Characteristics of Lower Respiratory Tract Escherichia Coli Isolates Colonizing and Infecting Mechanically Ventilated Intensive Care Patients: a French Multicenter Prospective Collection
Prospective, multicenter observational study to collect Escherichia coli (E. coli) isolates originating from mechanically ventilated intensive care unit (ICU) patients; in order to characterize phenotype and genotype of E. coli strains retrieved from the lower respiratory tract of ventilated patients.
Prospective, observational, multiple center study performed in 14 ICUs in France to collect
Escherichia coli (E. coli) isolates originating from mechanically ventilated intensive care
unit (ICU) patients; in order to characterize phenotype and genotype of E. coli strains
retrieved from the lower respiratory tract of ventilated patients. All E. coli isolates
identified in the microbiology lab and retrieved from a lung specimen (either tracheal
aspirate, bronchoalveolar lavage, or telescopic plugged catheter) originating from an ICU
patient will be kept, and stored at -80°C in brain-heart infusion broth containing glycerol
20 %. They will be then centralized in the investigators' research unit for further analysis
that includes determination of Antimicrobial susceptibility, E. coli phylotype , O-type, and
virulence factor gene content.
These isolates will be compared to those of two previously published collections, one from
the stools of community subjects, considered as commensal strains, the other from the blood
of bacteraemia patients.
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