No Condition, Focus: Metabolites of Alcohol Consumption Clinical Trial
Official title:
Circulating Metabolites Associated With Alcohol Intake in the European Prospective Investigation Into Cancer and Nutrition Cohort
Alcohol consumption is a risk factor for numerous health conditions and an important cause of death. Identifying metabolites associated with alcohol consumption may provide insights into the metabolic pathways through which alcohol may affect human health. The objective of this study is to investigate associations of alcohol consumption with circulating concentrations of 123 metabolites including amino acids, acylcarnitines, hexoses, biogenic amines, phosphatidylcholines, and sphingomyelins. For this purpose, the investigators use data from the European Prospective Investigation into Cancer and Nutrition (EPIC) study and applied a discovery and replication approach.
This study used data from 2,974 control participants from four case-control studies on
colorectal (n=491), hepatobiliary (n=327), kidney (n=635), and prostate cancer (n=1,521)
nested in the EPIC cohort, for which targeted metabolomics data had been acquired. Alcohol
consumption at recruitment was self-reported through dietary questionnaires. Metabolite
concentrations were measured by tandem mass spectrometry using the BIOCRATES AbsoluteIDQTM
p180 kit. Data were randomly divided into discovery (2/3) and replication (1/3) sets.
Multivariable linear regression models were used to evaluate confounder-adjusted associations
of ln-transformed alcohol consumption with Z-standardized ln-transformed residual metabolite
concentrations. Metabolites significantly related to alcohol intake in the discovery set (FDR
q-value<0.05) were further tested in the replication set (Bonferroni-corrected p-value<0.05).
Of the 72 metabolites significantly related to alcohol intake in the discovery set, 34
metabolites were also significant in the replication analysis, including three
acylcarnitines, the amino acid citrulline, four lysophosphatidylcholines, 13
diacylphosphatidylcholines, seven acyl-alkylphosphatidylcholines, and six sphingomyelins.
Associations with acylcarnitines and phosphatidylcholines were generally positive, while
mostly inverse associations were observed with citrulline and sphingomyelins.
This study adds novel knowledge regarding circulating metabolites associated with alcohol
consumption, and provides leads for further studies into the underlying biological
mechanisms. A better understanding of metabolic pathways affected by alcohol consumption may
contribute to the development of mechanism-tailored intervention strategies to prevent and
treat alcohol-related conditions. Furthermore, it may help to identify biomarkers of alcohol
consumption facilitating early preventive strategies in individuals at-risk for developing
alcohol-related morbidities.
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