Effect of Acheta Domesticus Supplementation on Post-Exercise Muscle Recovery in Recreationally Active Male

Muscle Soreness Clinical Trial

Official title:

Effect of Acheta Domesticus Supplementation on Post-Exercise Muscle Recovery in Recreationally Active Male

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT06096389
• Other study ID #: UM.TNC2/UMREC_2337
• Status: Recruiting
• Phase: N/A
• Start date: April 1, 2023
• Est. completion date: December 31, 2023

Study information

• Verified date: October 2023
• Source: University of Malaya
• Contact: Jerry Vanlin
• Phone: +60133248766
• Email: jerry.vanlin[@]gmail.com
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The goal of this clinical trial is to test the effect of house cricket (Acheta domesticus) supplementation for strength recovery in recreationally active males. The main question[s] it aims to answer are: whether house cricket powder could improve strength recovery and whether house cricket powder could be an alternative to commonly used protein supplement. Participants will do exercise (100 drop jumps) to induce fatigue and then they will consume either isocaloric carbohydrate, whey protein isolate or defatted house cricket powder for 4 days. Their strength will be measured every 24 hours for 4 days in order to observe the recovery.

Researcher will compare house cricket powder with isocaloric carbohydrate to see if the strength recovery is faster in house cricket powder group

Researcher will compare house cricket powder with whey protein isolate to see if the strength recovery is comparable.

Description:

Participants: recreationally active males Study type: double-blind randomized controlled trial

Supplement dose is tailored to each participant daily protein consumption. It aims to reach 1.4 g/kg body weight protein, hence participant is required to provide dietary record 3 days before the experiment and during the experiment. Subject will attend one familiarization 1 week before the experiment. During day one of the experiment, subject warmed up before the session. Then their baseline strength and jumping measurement will be taken. Afterwards, they will do 20 reps of drop jumps for 5 sets (each set separated by 2 minutes rest). Immediately after the exercise, they will consume either whey, house cricket or maltodextrin. 10 minutes afterwards, they will undergo another strength and jumping measurement. 24, 48 and 72 hours after the exercise, subject will come for strength and jumping post-test after consuming the supplement. They are also required to fill in a visual analogue scale and 7-points Likert scale form regarding their pain and soreness perception.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 33
• Est. completion date: December 31, 2023
• Est. primary completion date: November 30, 2023
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 30 Years

Eligibility

Inclusion Criteria:

• 5-10 hours of exercise per week

Exclusion Criteria:

• smoking

• drinking alcohol

• history of surgical/medical procedure that could affect the study

• food allergy

• in medication

• have participated in similar studies before

Related Conditions & MeSH terms

• Muscle Soreness
• Myalgia

Intervention

Other:
• 100 Drop Jumps
100 drop jumps from 60 cm height. Divided into 5 sets of 20 reps

Dietary Supplement:
• Whey supplementation
Consume whey protein isolate based on subjects' daily protein intake
• Carbohydrate supplementation
Consume carbohydrate (isocaloric to whey)
• House cricket supplementation
Consume defatted house cricket powder based on subjects' daily protein intake

Locations

Country	Name	City	State
Malaysia	Faculty of Sports and Exercise Sciences	Kuala Lumpur	Federal Territory Of Kuala Lumpur

Sponsors (1)

Lead Sponsor	Collaborator
University of Malaya

Country where clinical trial is conducted

Malaysia, 

References & Publications (37)

Abaidia AE, Delecroix B, Leduc C, Lamblin J, McCall A, Baquet G, Dupont G. Effects of a Strength Training Session After an Exercise Inducing Muscle Damage on Recovery Kinetics. J Strength Cond Res. 2017 Jan;31(1):115-125. doi: 10.1519/JSC.0000000000001479. — View Citation

Abbott W, Brett A, Cockburn E, Clifford T. Presleep Casein Protein Ingestion: Acceleration of Functional Recovery in Professional Soccer Players. Int J Sports Physiol Perform. 2019 Mar 1;14(3):385-391. doi: 10.1123/ijspp.2018-0385. Epub 2019 Feb 17. — View Citation

Agriculture Organization of the United Nations, World Health Organization, & University, U. N. (2007). Protein and amino acid requirements in human nutrition : report of a joint FAO/WHO/UNU expert consultation. World Health Organization. https://apps.who.int/iris/handle/10665/43411

Awobusuyi TD, Pillay K, Siwela M. Consumer Acceptance of Biscuits Supplemented with a Sorghum-Insect Meal. Nutrients. 2020 Mar 25;12(4):895. doi: 10.3390/nu12040895. — View Citation

Baiano A. (2020). Edible insects: an overview on nutritional characteristics, safety, farming, production technologies, regulatory framework, and socio-economic and ethical implications. Trends in Food Science & Technology. 100:35-50

Borgenvik M, Apro W, Blomstrand E. Intake of branched-chain amino acids influences the levels of MAFbx mRNA and MuRF-1 total protein in resting and exercising human muscle. Am J Physiol Endocrinol Metab. 2012 Mar 1;302(5):E510-21. doi: 10.1152/ajpendo.00353.2011. Epub 2011 Nov 29. — View Citation

Brogan, E. N., Park, Y. L., Matak, K. E., & Jaczynski, J. (2021). Characterization of protein in cricket (Acheta domesticus), locust (Locusta migratoria), and silk worm pupae (Bombyx mori) insect powders. Lwt-Food Science and Technology, 152, Article 112314. https://doi.org/10.1016/j.lwt.2021.112314

Brown MA, Stevenson EJ, Howatson G. Whey protein hydrolysate supplementation accelerates recovery from exercise-induced muscle damage in females. Appl Physiol Nutr Metab. 2018 Apr;43(4):324-330. doi: 10.1139/apnm-2017-0412. Epub 2017 Nov 6. — View Citation

Buckley JD, Thomson RL, Coates AM, Howe PR, DeNichilo MO, Rowney MK. Supplementation with a whey protein hydrolysate enhances recovery of muscle force-generating capacity following eccentric exercise. J Sci Med Sport. 2010 Jan;13(1):178-81. doi: 10.1016/j.jsams.2008.06.007. Epub 2008 Sep 2. — View Citation

Buford TW, Kreider RB, Stout JR, Greenwood M, Campbell B, Spano M, Ziegenfuss T, Lopez H, Landis J, Antonio J. International Society of Sports Nutrition position stand: creatine supplementation and exercise. J Int Soc Sports Nutr. 2007 Aug 30;4:6. doi: 10.1186/1550-2783-4-6. No abstract available. — View Citation

Cooke MB, Rybalka E, Stathis CG, Cribb PJ, Hayes A. Whey protein isolate attenuates strength decline after eccentrically-induced muscle damage in healthy individuals. J Int Soc Sports Nutr. 2010 Sep 22;7:30. doi: 10.1186/1550-2783-7-30. — View Citation

Cormack SJ, Newton RU, McGuigan MR, Doyle TL. Reliability of measures obtained during single and repeated countermovement jumps. Int J Sports Physiol Perform. 2008 Jun;3(2):131-44. doi: 10.1123/ijspp.3.2.131. — View Citation

de Matos FM, Novelli PK, de Castro RJS. Enzymatic hydrolysis of black cricket (Gryllus assimilis) proteins positively affects their antioxidant properties. J Food Sci. 2021 Feb;86(2):571-578. doi: 10.1111/1750-3841.15576. Epub 2021 Jan 12. — View Citation

Eddens L, Browne S, Stevenson EJ, Sanderson B, van Someren K, Howatson G. The efficacy of protein supplementation during recovery from muscle-damaging concurrent exercise. Appl Physiol Nutr Metab. 2017 Jul;42(7):716-724. doi: 10.1139/apnm-2016-0626. Epub 2017 Feb 15. — View Citation

Etheridge T, Philp A, Watt PW. A single protein meal increases recovery of muscle function following an acute eccentric exercise bout. Appl Physiol Nutr Metab. 2008 Jun;33(3):483-8. doi: 10.1139/H08-028. — View Citation

Hansen M, Bangsbo J, Jensen J, Bibby BM, Madsen K. Effect of whey protein hydrolysate on performance and recovery of top-class orienteering runners. Int J Sport Nutr Exerc Metab. 2015 Apr;25(2):97-109. doi: 10.1123/ijsnem.2014-0083. Epub 2014 Jul 14. — View Citation

Hermans WJH, Senden JM, Churchward-Venne TA, Paulussen KJM, Fuchs CJ, Smeets JSJ, van Loon JJA, Verdijk LB, van Loon LJC. Insects are a viable protein source for human consumption: from insect protein digestion to postprandial muscle protein synthesis in vivo in humans: a double-blind randomized trial. Am J Clin Nutr. 2021 Sep 1;114(3):934-944. doi: 10.1093/ajcn/nqab115. — View Citation

Hilkens L, De Bock J, Kretzers J, Kardinaal AFM, Floris-Vollenbroek EG, Scholtens PAMJ, Horstman AMH, van Loon LJC, van Dijk JW. Whey protein supplementation does not accelerate recovery from a single bout of eccentric exercise. J Sports Sci. 2021 Feb;39(3):322-331. doi: 10.1080/02640414.2020.1820184. Epub 2020 Oct 5. — View Citation

Kreider RB, Campbell B. Protein for exercise and recovery. Phys Sportsmed. 2009 Jun;37(2):13-21. doi: 10.3810/psm.2009.06.1705. — View Citation

Kritikos S, Papanikolaou K, Draganidis D, Poulios A, Georgakouli K, Tsimeas P, Tzatzakis T, Batsilas D, Batrakoulis A, Deli CK, Chatzinikolaou A, Mohr M, Jamurtas AZ, Fatouros IG. Effect of whey vs. soy protein supplementation on recovery kinetics following speed endurance training in competitive male soccer players: a randomized controlled trial. J Int Soc Sports Nutr. 2021 Mar 16;18(1):23. doi: 10.1186/s12970-021-00420-w. — View Citation

Navarro Del Hierro J, Gutierrez-Docio A, Otero P, Reglero G, Martin D. Characterization, antioxidant activity, and inhibitory effect on pancreatic lipase of extracts from the edible insects Acheta domesticus and Tenebrio molitor. Food Chem. 2020 Mar 30;309:125742. doi: 10.1016/j.foodchem.2019.125742. Epub 2019 Oct 25. — View Citation

Nino MC, Reddivari L, Ferruzzi MG, Liceaga AM. Targeted Phenolic Characterization and Antioxidant Bioactivity of Extracts from Edible Acheta domesticus. Foods. 2021 Sep 28;10(10):2295. doi: 10.3390/foods10102295. — View Citation

Oonincx DG, van Itterbeeck J, Heetkamp MJ, van den Brand H, van Loon JJ, van Huis A. An exploration on greenhouse gas and ammonia production by insect species suitable for animal or human consumption. PLoS One. 2010 Dec 29;5(12):e14445. doi: 10.1371/journal.pone.0014445. — View Citation

Orkusz A. Edible Insects versus Meat-Nutritional Comparison: Knowledge of Their Composition Is the Key to Good Health. Nutrients. 2021 Apr 6;13(4):1207. doi: 10.3390/nu13041207. — View Citation

Osimani, A., Milanovic, V., Cardinali, F., Roncolini, A., Garofalo, C., Clementi, F., Pasquini, M., Mozzon, M., Foligni, R., Raffaelli, N., Zamporlini, F., & Aquilanti, L. (2018). Bread enriched with cricket powder (Acheta domesticus): A technological, microbiological and nutritional evaluation. Innovative Food Science & Emerging Technologies, 48, 150-163. https://doi.org/https://doi.org/10.1016/j.ifset.2018.06.007

Paul, A., Frederich, M., Megido, R. C., Alabi, T., Malik, P., Uyttenbroeck, R., Francis, F., Blecker, C., Haubruge, E., Lognay, G., & Danthine, S. (2017). Insect fatty acids: A comparison of lipids from three Orthopterans and Tenebrio molitor L. larvae. Journal of Asia-Pacific Entomology, 20(2), 337-340. https://doi.org/https://doi.org/10.1016/j.aspen.2017.02.001

Placentino U, Sogari G, Viscecchia R, De Devitiis B, Monacis L. The New Challenge of Sports Nutrition: Accepting Insect Food as Dietary Supplements in Professional Athletes. Foods. 2021 May 18;10(5):1117. doi: 10.3390/foods10051117. — View Citation

Stull VJ, Finer E, Bergmans RS, Febvre HP, Longhurst C, Manter DK, Patz JA, Weir TL. Impact of Edible Cricket Consumption on Gut Microbiota in Healthy Adults, a Double-blind, Randomized Crossover Trial. Sci Rep. 2018 Jul 17;8(1):10762. doi: 10.1038/s41598-018-29032-2. — View Citation

Tang JE, Moore DR, Kujbida GW, Tarnopolsky MA, Phillips SM. Ingestion of whey hydrolysate, casein, or soy protein isolate: effects on mixed muscle protein synthesis at rest and following resistance exercise in young men. J Appl Physiol (1985). 2009 Sep;107(3):987-92. doi: 10.1152/japplphysiol.00076.2009. Epub 2009 Jul 9. — View Citation

Tipton KD, Elliott TA, Cree MG, Wolf SE, Sanford AP, Wolfe RR. Ingestion of casein and whey proteins result in muscle anabolism after resistance exercise. Med Sci Sports Exerc. 2004 Dec;36(12):2073-81. doi: 10.1249/01.mss.0000147582.99810.c5. — View Citation

Trommelen J, van Loon LJ. Pre-Sleep Protein Ingestion to Improve the Skeletal Muscle Adaptive Response to Exercise Training. Nutrients. 2016 Nov 28;8(12):763. doi: 10.3390/nu8120763. — View Citation

Udomsil, N., Imsoonthornruksa, S., Gosalawit, C., & Ketudat-Cairns, M. (2019). Nutritional values and functional properties of house cricket (Acheta domesticus) and field cricket (Gryllus bimaculatus). Food Science and Technology Research, 25(4), 597-605. https://doi.org/10.3136/fstr.25.597

van Huis, A., van Itterbeeck, J., Klunder, H., Mertens, E., Halloran, A., Muir, G., Vantomme, P. (2013). Edible insects: future prospect for food and feed security. Food and Agriculture Organization of the United Nations.

Vangsoe MT, Joergensen MS, Heckmann LL, Hansen M. Effects of Insect Protein Supplementation during Resistance Training on Changes in Muscle Mass and Strength in Young Men. Nutrients. 2018 Mar 10;10(3):335. doi: 10.3390/nu10030335. — View Citation

Vangsoe MT, Thogersen R, Bertram HC, Heckmann LL, Hansen M. Ingestion of Insect Protein Isolate Enhances Blood Amino Acid Concentrations Similar to Soy Protein in A Human Trial. Nutrients. 2018 Sep 22;10(10):1357. doi: 10.3390/nu10101357. — View Citation

Wilborn CD, Taylor LW, Outlaw J, Williams L, Campbell B, Foster CA, Smith-Ryan A, Urbina S, Hayward S. The Effects of Pre- and Post-Exercise Whey vs. Casein Protein Consumption on Body Composition and Performance Measures in Collegiate Female Athletes. J Sports Sci Med. 2013 Mar 1;12(1):74-9. eCollection 2013. — View Citation

Witard OC, Jackman SR, Kies AK, Jeukendrup AE, Tipton KD. Effect of increased dietary protein on tolerance to intensified training. Med Sci Sports Exerc. 2011 Apr;43(4):598-607. doi: 10.1249/MSS.0b013e3181f684c9. — View Citation

* Note: There are 37 references in all — Click here to view all references

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Strength	Isokinetic knee extension and flexion, maximum knee isometric contraction	72 hours
Primary	Power	Countermovement jump, squat jump	72 hours
Primary	Pain Perception	10 cm visual analogue scale (min 0 = no soreness, max 10 = worst imaginable muscle soreness) & 7 Points Likert Scale (min 0 = no soreness, max 6 = a severe pain that limits my ability to move)	72 hours