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FOS Immunohistochemical Staining of Colorectal Cancer and Its Adjacent Tissues

Colorectal Cancer Clinical Trial

Official title:
Monkeypox and Cancer: a Pan-cancer Based Multi-omics Analysis and Single Cell Sequencing Analysis-Experimental Studies

Clinical Trial Summary

Colorectal cancer tissue sections were obtained according to the inclusion criteria. The formalin was used to immersed all cancer specimens. And tissues were cut to 5 μm thickness and placed on glass slides before staining. Endogenous peroxidase activity was inhibited and blocked by de-paraffinizing, rehydrating, and using 5% bovine serum albumin at 37ºC for 30 min. The treated sections were incubated with anti-FOS (promab 30360) at 4ºC overnight and washed three times with PBS. After that, it is required that incubation with secondary anti-peroxidation sunflower at 37ºC for 30 minutes. After washing three times again with PBS, the sections were developed in diaminobenzidine and microscopic images were made by light microscopy.

Clinical Trial Description

n/a

Study Design

Related Conditions & MeSH terms

Clinical Trial Details
NCT number NCT05698082
Study type Interventional
Source Cancer Hospital of Guangxi Medical University
Contact Xiaoliang Huang, Dr.
Phone 0086-13617713823
Email xiaoliang@sr.gxmu.edu.cn
Status Recruiting
Phase Phase 2/Phase 3
Start date December 1, 2020
Completion date January 1, 2025
View Details
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