Isothiocyanates and Metabolic Health

Metabolic Disturbance Clinical Trial

— IMH  

Official title:

Isothiocyanates and Metabolic Health

Clinical Trial Details — Status: Active, not recruiting

Administrative data

• NCT number: NCT05070585
• Other study ID #: SwedishSchoolSport
• Status: Active, not recruiting
• Phase: Phase 1/Phase 2
• Start date: October 20, 2021
• Est. completion date: December 5, 2023

Study information

• Verified date: December 2022
• Source: The Swedish School of Sport and Health Sciences
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Many sprouts, for example broccoli sprouts, contain substances that have shown to have protective effects, both against oxidative stress and against carcinogenic substances.

In this study we will investigate the activation of the body's antioxidant defense system and effect on blood lactate and glucose after 1 week of isothiocyanate rich sprout supplementation. The subjects will perform maximal and submaximal work test, blood sampling, muscle biopsies and blood pressure monitoring post supplementation. We hypothesize that 1 week of supplementation of isothiocyanate rich sprouts alters blood lactate and glucose metabolism and activates the body's antioxidant defense system.

Description:

Many sprouts, for example broccoli sprouts, contain high levels of glucosinolates, which can be converted to the bioactive substances isothiocyanates. This conversion is assisted by another plant enzyme, myrosinase, which is released when the plant is mechanically processed. It is believed that this reaction is part of the plant's natural defense against pest insects and other animals that attack the plant in the early developmental stage. In cell and animal studies supplementation with isothiocyanates has been shown to induce the protein Nrf2 which is a so-called "Master regulator" of the body's antioxidant defenses and activates more than 200 genes that together provide a strong protection against oxidative and mutagenic stress.

In this study we will investigate Nrf2 activation in human skeletal muscle and effect on lactate-, glucose metabolism after 1 week of isothiocyanate rich sprout supplementation. The study is a double-blinded, randomized, placebo controlled, cross-over study with 4 weeks wash-out between the interventions. We will perform maximal- and submaximal work test and monitor blood glucose and lactate during the tests. Also, in the post intervention phases we will perform muscle biopsies, glucose tolerance tests and monitor blood pressure.

The hypothesis is that 1 week of isothiocyanate rich sprout supplementation activates Nrf2 in human skeletal muscle and alters blood lactate and glucose metabolism in rest and during exercise.

Recruitment information / eligibility

• Status: Active, not recruiting
• Enrollment: 12
• Est. completion date: December 5, 2023
• Est. primary completion date: December 1, 2022
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 45 Years

Eligibility

Inclusion Criteria:

• Healthy volunteer

• 18-45 years old

Exclusion Criteria:

• Any chronic disease

• Inability to perform cycling exercise

• Allergic to anesthesia

Related Conditions & MeSH terms

• Metabolic Disturbance

Intervention

Dietary Supplement:
• Isothiocyanate rich sprouts
Isothiocyanate rich sprout juice consumption for 1 week
• Placebo Juice
Sprout juice devoid of isothiocyanates for 1 week

Locations

Country	Name	City	State
Sweden	Swedish School of Sports and Health Sciences	Stockholm

Sponsors (1)

Lead Sponsor	Collaborator
The Swedish School of Sport and Health Sciences

Country where clinical trial is conducted

Sweden, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Blood lactate	Changes in blood lactate during exercise	Acute during sub maximal exercise
Primary	Nrf2 activation	Increased Nrf2 protein in human skeletal muscle	Acute after 1 week of supplementation
Primary	Glucose regulation	Improved continuous glucose control	Throughout 1 week of supplementation
Secondary	Glucose tolerance test	Improved glucose regulation in rest	120 minutes
Secondary	Clinic blood pressure	Blood pressure regulation	Acute after 1 week of supplementation