Clinical Trial Details
— Status: Not yet recruiting
Administrative data
| NCT number |
NCT05348746 |
| Other study ID # |
Doctors with Africa CUAMM |
| Secondary ID |
|
| Status |
Not yet recruiting |
| Phase |
|
| First received |
|
| Last updated |
|
| Start date |
May 1, 2022 |
| Est. completion date |
April 1, 2024 |
Study information
| Verified date |
April 2022 |
| Source |
Doctors with Africa - CUAMM |
| Contact |
Francesco Di Gennaro, MD |
| Phone |
+393924804707 |
| Email |
cicciodigennaro[@]yahoo.it |
| Is FDA regulated |
No |
| Health authority |
|
| Study type |
Observational
|
Clinical Trial Summary
Objective: Measure incidence of malaria and malaria-related outcomes, evaluating potential
impact of the SARS-CoV2 epidemic and of antimalarial resistance in Oyam and Kole district,
Uganda with focus on pregnant women.
Study design: Facility-based, prospective, observational study.
Study population: All pregnant women at any gestational age presenting to the Aber Hospitals
during the study period both at the emergency department or the Ante-Natal Care (ANC) clinic
will be eligible to participate in this study.
Methods: Women will be recruited at ANC visits and at the emergency department and screened
against the inclusion criteria. Women will be followed until delivery and evaluated during
the consecutives ANC visits. Outcomes will be assessed at the delivery or/and at the
discharge if admitted to the hospital for any other causes related with the pregnancy or the
malaria. Also, a subpopulation of nonpregnant individuals diagnosed with malaria will be
recruited for resistance detection.
Main study parameters/primary endpoints: Incidence of malaria and malaria-related adverse
outcomes; impact of the COVID-19 pandemic on malaria care; prevalence of antimalarial
resistance against artemisinin derivatives and sulphadoxine-pyrimethamine.
Description:
1. INTRODUCTION
1.1. Malaria epidemiology and global trends Globally, there were an estimated 241 million
malaria cases in 2020 in 85 malaria endemic countries, increasing from 227 million in 2019,
with most of this increase coming from countries in the WHO African Region.
Although it still represents a major threat for public health, over the last twenty years
tremendous progress has been made on malaria control, averting an estimate of 1.5 billion
cases and saving 7.6 million lives. Malaria mortality fell by 60% over the period from 2000
to 2019, especially in Africa, where political commitment, ignited by the 2000 Abuja
declaration has been the key to success. Along with strong political leadership, these data
outline the potential benefit in malaria control and prevention brought by public health
programs. However, despite the astonishing results achieved in the last two decades, in 2020,
COVID-19 pandemic added a crucial challenge in the fight against the disease, posing a major
risk for Low and Middle Income Countries (LMICs). In fact, in sub-Saharan countries, along
with the substantial danger posed directly to the already fragile healthcare systems, malaria
control has been threatened by supply-chain interruptions and by individual decision-making
adaptation. Indeed, malaria control relies heavily on individual choice to seek care, and
early messaging targeted on reducing SARS-CoV2 transmission advised people to stay home in
case of fever. According to geostatistical models, these factors may contribute to an
estimated increase, in a worst-case scenario, of 21% malaria cases and nearly double
malaria-related deaths in the upcoming years. Furthermore, the true COVID-19 burden in
sub-Saharan countries is unknown and, according to seroprevalence studies, it is likely to be
largely underestimated . All these factors, according to the latest WHO World Malaria Report,
contributed to a reverse of the reducing trend in malaria case incidence (59 cases per 1000
people at risk in 2020, compared to 56 in 2019) and malaria deaths, that passed from 534,000
in 2019 to 602,000 in 2020.
Other substantial challenges towards malaria eradication are drug-resistance, acquisition of
mutations impairing diagnosis - especially those affecting the sensitivity of
rapid-diagnostic tests - access to treatment and supply of malaria prevention tools such as
insecticide-treated mosquito nets (ITN), Indoor Residual Spraying (IRS), Seasonal Malaria
Chemoprevention (SMC) and Intermittent Preventive Treatment for pregnant women (IPTp).
1.3 RESEARCH QUESTIONS
1. How COVID-19 pandemic impacted malaria care and malaria-related prevention attitudes
among pregnant women in Oyam and Kole districts?
2. What is the prevalence of clinical resistance against SP and artemisinin in Oyam and
Kole districts?
3. How COVID-19 pandemic and antimalarial resistance impacted malaria-related outcomes in
pregnant women?
1.4 OBJECTIVES 1.4.1 Main Objective The general aim of the study is to assess the impact of
Antimalarial Resistance and COVID-19 Pandemic on Malaria Prevention, Diagnosis and Treatment
among Pregnant Women in Northern Uganda.
1.4.2 Specific Objectives
1. Assess the impact of the COVID-19 pandemic on malaria prevention and control practices
for pregnant women in terms of: i) access to antenatal care visits, ii) administration
of IPTp, iii) healthcare seeking behavior in case of fever, iv) healthcare provider
compliance with national and international guidelines, v) supply and use of
insecticide-treated nets and indoor residual spraying in Oyam and Kole district for the
period March 2022 to June 2023.
2. Assess incidence of symptomatic and severe malaria in the study cohort in Oyam and Kole
district for the period March 2022 to June 2023.
3. Evaluate the determinants of malaria prevention and control compliance in pregnant women
in Oyam and Kole district for the period March 2022 to June 2023.
4. Assess incidence of malaria-related adverse maternal and fetal outcomes (maternal
anemia, maternal mortality, abortion, low birthweight, prematurity, perinatal mortality,
placental malaria) in Oyam and Kole district for the period March 2022 to June 2023.
5. Assess ideation and knowledge regarding SARS-CoV2 infection and malaria among preganant
women in Oyam and Kole district for the period March 2022 to June 2023.
6. Assess the prevalence of asymptomatic P. falciparum infection in the study cohort in
Oyam and Kole district for the period March 2022 to June 2023.
7. Evaluate the status of circulating dhfr and dhps haplotypes by describing polymorphisms
in the P. falciparum dhfr and dhps genes and estimate the prevalence of dhfr / dhps
combined mutant haplotypes associated with pyrimethamine and sulfadoxine resistance in
the study cohort and in a population of patients diagnosed with malaria in Oyam and Kole
district for the period June 2022 to May 2024.
8. Evaluate the emergence and local spread of artemisinin resistance by means of the
analysis of polymorphisms in the P. falciparum kelch 13 gene in the study cohort and in
a population of patients diagnosed with malaria in Oyam and Kole district for the period
June 2022 to May 2024.
2.0 METHOD AND MATERIALS 2.1 STUDY DESIGN: This will be a health facility based
prospective observational study, using quantitative methods of data collection.
Semi-structured questionnaires will be administered to collect the data. The data will
be collected prospectively, following a cohort of pregnant women presenting to antenatal
care visits until delivery. Also, a subpopulation of nonpregnant individuals diagnosed
with malaria will be recruited for resistance detection. Collected data will explore the
attitude towards malaria prevention during the COVID-19 pandemic, in terms of access to
antenatal care visits, administration of IPTp, healthcare seeking behaviour in case of
fever, use of insecticide-treated nets and Indoor residual spraying, ideation and
knowledge in regard to SARS-CoV2 infection and malaria.
2.2 STUDY SETTING The study will be conducted at Aber hospital and selected Health
Facilities in Oyam and Kole districts, Lango region. Both the hospital and the district
Health Centers are managed by the NGO Doctors with Africa CUAMM.
St. John's Hospital Aber is a private, non-profit, community hospital owned by the Roman
Catholic Diocese of Lira and is accredited by the Uganda Catholic Medical Bureau. The
hospital is administered by Sisters of Mary, Mother of the Church, a religious
congregation. The hospital has a capacity of 220 beds. Aber Hospital has annual
outpatient department attendance of 27,827 contacts, 6,800 admissions with a bed
occupancy rate of about 40% and 1,700 average annual maternal deliveries with a cesarean
section rate of 21%.
Oyam district it is located in the Northern part of Uganda. It has it total population
of 366,200 people. It is bordered by the Districts of Gulu in the North, Kiryandongo in
the South West, Nwoya in the West, Apac in the South and Kole in the East. The district
comprises of 12 sub counties i.e. Loro, Minakulu, Aber, Acaba, Ngai, Iceme, Otwal, Abok,
Myene, Aleka, Kamdini, and Oyam Town Council. Kole District is bordered by Lira District
to the east, Apac District to the south and Oyam District to the west and north. Kole,
the district capital, is located approximately 28 kilometres (17 mi), by road, northwest
of Lira, the largest city in the sub-region. Kole district is subdivided into the
following sub-counties: Aboke, Akalo, Alito, Ayer, Bala, Okwerodot, Ayer Town Council.
2.3 SAMPLING PROCEDURE AND SAMPLE SIZE ESTIMATION 2.3.1 Sample size estimation
Resistance detection
Considering that:
- the expected prevalence of parasitaemia among asymptomatic pregnant women is 5%
- the expected prevalence of parasitaemia among pregnant women who are febrile is 13%
- in 2019, the prevalence of P. falciparum carrying gene polymorphisms associated
with resistance was 20% for artemisinin derivates and 16% for SP.
In order to estimate the proportion positive for P. falciparum with a 2% absolute
precision, at a power of 80% and a 5% level of significance (two sided), a minimum of
800 pregnant women will be required, allowing for a 10% loss to follow-up. The
investigators need to recruit 1200 - 1400 women with unknown parasitological status to
detect around 20 resistances, even considering an increasing trend.
To support the achievement of the minimum sample size, the researcher need to recruit a
subpopulation of nonpregnant, microscopically confirmed malaria patients from Aber
Hospital. This additional recruitment will be performed by consecutive enrollment until
the attainment of the minimum sample size of 300 microscopically confirmed, P.
falciparum infected samples.
Cohort of pregnant women Study population will be recruited by consecutive sampling,
from 1st May 2022 to 30 June 2023.
2.3.2 Sampling procedure
The health facilities will be selected by purposive sampling. The sampling will be based
on the following criteria:
- laboratory capacity
- presence of an experienced microscopist
- antenatal care visit volumes of at least 50 visits per month
- quality of service as per periodic CUAMM assessment
2.3.3 Selection of participants Participants coming to the sampled healthcare facilities
who meet the eligibility criteria will be included in the study. Consecutive enrollment
of participants will be undertaken up to when the minimum sample size required for the
study will be met.
2.7 STUDY PROCEDURES AND TIMELINE
2.7.1 Study procedures Microscopy Thick and thin blood smears will be stained with 2%
Giemsa and read by experienced laboratory technologists. Parasite densities will be
calculated by counting the number of asexual parasites per 200 leukocytes (or per 500
leukocytes, if the count is <10 asexual parasites/200 leukocytes), assuming a leukocyte
count of 8,000/µl. A blood smear will be considered negative when the examination of 100
high power fields does not reveal asexual parasites. Gametocytemia will also be
determined from thick smears. Thin smears w1ill be used for parasite species
identification.
Molecular diagnosis and Plasmodium species confirmation The blood samples of the
patients will be collected using filter paper (Whatman 3 MM) during admission to the
healthcare facility. The dried blood spots (DBSs) will be collected through a finger
prick (three drops of blood per participant) on filter papers which will be dried and
kept in plastic bags with desiccant and stored in boxes in a cool dry place at room
temperature before being transferred at the ISS for molecular diagnosis and drug
resistance analysis.
Shipment of the Blood samples The collected blood samples will be shipped to Italy for
advanced Polymorphism analysis. During shipment, all the samples will be stored in a
dry, cool place at room temperature to the Italian National Institute of Health
(Istituto Superiore di Sanità, ISS).
Advanced Analysis of Polymorphism Total genomic DNA will be extracted from filter blots
(3MM Whatman) using the PureLink Genomic DNA Kits-Invitrogen, according to the
manufacturer's recommendation. Parasite identification is based on nested PCR assay
targeting the 18S rRNA gene. The 18S rRNA gene is used as a target since it contains
both highly conserved and variable regions for each Plasmodium species. The
genus-specific PCR will be followed by Plasmodium species-specific PCR amplification.
Amplicons from the second PCR will be separated by electrophoresis on a 2% agarose gel
and stained with ethidium bromide for visualization using ultraviolet
trans-illumination. The presence of parasitaemia will be confirmed when the expected
band size corresponding to P. falciparum, P. vivax, P. malariae and or P. ovale will be
identified.
Assessment of Plasmodium falciparum drug resistance. Target P. falciparum drug
resistance genes: Pfk13 propeller, Pfdhfr and Pfdhps. The polymorphisms analysis of the
propeller domain of the Pfk13 gene will be performed by PCR amplifications and
subsequent sequencing. Analysis of Pfdhfr gene at codons 51, 59, 108 and Pfdhps gene at
codon positions 436, 437, 540, 581, 613 will be done by means of amplifications and
subsequent Sanger sequencing. Commercial oligonucleotide primer pairs for Pfk13 will be
obtained based on the published article by Taylor et al., whereas for the analysis of
dhfr and dhps genes primer pairs will be obtained based on the published article by
Menegon et al. [31]. The obtained sequences will be compiled and analyzed by Accelrys DS
Gene software. PlasmoDB gene identification no. PF3D7_1343700 (P. falciparum 3D7 strain)
will be used as reference in the numbering of nucleotide and amino acid positions.
Molecular studies will be performed only for research purposes and will have no impact
on the clinical management of study patients.
To enhance local capacity building, one laboratory person from Aber hospital will attend
a two weeks exposure at the reference laboratory in Italy.
Study time points TIME POINT STUDY PROCEDURES AND DATA COLLECTION RECRUITMENT (ANC
VISIT) - Full questionnaire administered by a trained health care worker to collect
information on demographic (eg. age, area of residence), socioeconomic factors (eg.
education, occupation), number and outcome of previous pregnancies, date of last
menstruation, bed net ownership and use, adherence to malaria chemoprevention, reasons
for non-adherence, malaria behaviors, barriers to administration of IPTp, use of ITNs,
perceived impact of COVID-19 will be administered.
- Clinical examination assessing the general wellbeing and nutritional status of the
woman, along with routine measurements (including weight, height, auscultation,
blood pressure and temperature). Gestational age will be assessed, when available,
by obstretic ultrasound and, if unavailable, by pelvis examination performed by
experienced midwives
- Collection of blood sample that will be analyzed as follows:
- Malaria diagnostic test with microscopy and, when positive, parasite count.
Positive samples will be sent to the ISS. Molecular diagnosis will be
performed to confirm microscopy results and to discriminate between Plasmodium
species. Plasmodium falciparum positive samples will be analyzed for detection
of single nucleotide polymorphisms in P. falciparum genes associated with
artemisinin and SP resistance. Women diagnosed with malaria will be treated
and followed according to Uganda Clinical Guidelines, and parasitemia will be
reassessed at day 3.
- HIV diagnostic test.
- Hemoglobin levels. All women found to be HIV+ at study entry will be referred
for further evaluation and treatment.
ANC VISITS
- Physical examination
- Follow-up survey, investigating history of fever, malaria-related prevention
attitudes (compliance with IPTp, use of ITN) and eventual barriers in care-seeking
due to the COVID-19 pandemic.
- Malaria diagnostic test with microscopy and, when positive, parasite count.
Positive samples will be sent to the ISS. Molecular diagnosis will be performed to
confirm microscopy results and to discriminate between Plasmodium species.
Plasmodium falciparum Positive samples will be analyzed for detection of single
nucleotide polymorphisms in P. falciparum genes associated with artemisinin and SP
resistance. Women diagnosed with malaria will be treated and followed according to
Uganda Clinical Guidelines, and parasitemia will be reassessed at day 3.
ANY SPONTANEOUS VISITS TO THE HOSPITAL RELATED WITH PREGNANCY AND/OR MALARIA
- standardized history
- physical exam including temperature, pulse, and blood pressure measurement.
- Participants who are febrile (tympanic temperature > 38.0˚C) or report history of
fever in the past 24 hours will have blood obtained by finger prick for a thick
blood smear. If the thick blood smear is positive, the patient will be diagnosed
with malaria. If the thick blood smear is negative, the patient will be managed by
study physicians for a non-malarial febrile illness. If the patient is afebrile and
does not report a recent fever, a thick blood smear will not be obtained, except
when following routine testing schedules.
- In patients with positive microscopy for P. falciparum, the first, pre-therapy
blood sample collected as DBS will be sent for molecular diagnosis confirmation and
for genetic analysis of antimalarial resistance to ISS. Furthermore, in those
patients, parasitemia will be reassessed at day 3, as per WHO protocol.
- Recruitment of the subpopulation of non-pregnant individuals will be undertaken at
this time point.
DELIVERY
- Delivery information: study staff will document details of the delivery, including
date and time, type of delivery, estimated blood loss and any maternal, obstetrical
or neonatal complications.
- Fetal outcome.
- Infant information: Apgar score and birth weight with calibrated scales. At the
time of delivery, women will undergo repeat rapid HIV testing based on national
guidelines. If women are found to have become HIV-infected during pregnancy, both
the mother and their newborn will be withdrawn from the study and immediately
referred for care following local prevention of mother-to-child transmission
guidelines.
2.8 DATA COLLECTION AND MANAGEMENT 2.8.1 Data collection tools Data will be collected
using semi-structured questioners and managed using REDCap electronic data capture tools
hosted at "Catholic University of the Sacred Heart", Rome, Italy, REDCap (Research
Electronic Data Capture) is a secure, web-based software platform designed to support
data capture for research studies, providing 1) an intuitive interface for validated
data capture; 2) audit trails for tracking data manipulation and export procedures; 3)
automated export procedures for seamless data downloads to common statistical packages;
and 4) procedures for data integration and interoperability with external sources.
2.8.2 Management of the Anti-malarial resistance data Data will be recorded on standard
study data collection forms and will be reviewed for accuracy and completion. Upon
resolution of data forms errors/missing values, the form will be ready for data entry.
The obtained results will be entered into a database. A database will be developed to
accommodate data entry and management of the study's data. The database will be created
with a standard data management software package, such as Microsoft Access. A file for
each study form will be created. The analysis of the sequenced samples will produce a
number of standardized reports: separate Excel datasheets and maps for associated drug
resistance SNPs and all other SNPs (exonic and intronic). In addition, individual sample
variant call format (VCF) files will be generated.
2.8.3 COVID-19 Mitigation Measures:
The investigators know that the COVID-19 risk will continue for most of the study
duration. All study procedures including data collection and management activities will
be implemented with this understanding in mind and the following COVID-19 risk
mitigation measures will be followed:
- All study staff will be oriented on COVID-19- standard operation procedures (SOPs)
as communicated by Ministry of Health (MOH)
- The orientation meetings will follow the MOH recommended number of participant in
line with the COVID-19 prevention protocols.
- The investigators shall regularly use the zoom platform for online meetings to
update on study progress across the study sites
- The study staff and participants will be provided with COVID-19 personal protective
equipment (Mask, Hand sanitisers, and hand washing soap) to use when implementing
the study activities
- COVID-19 prevention protocols will be published, displayed and followed in all
study activity areas and attention of participants will be drawn to these protocols
as part of the introductory communication also to ensure continuation of access to
essential services.
- All study staff and participants will be provided with adequate information and
encouraged to take the COVID-19 vaccination as recommended by MOH
2.11 ETHICAL CONSIDERATIONS A study protocol will be submitted for approval to the Lacor
Hospital research Ethics committee and the Uganda National Council of Science and
Technology (UNCST). Pregnant women will be asked for written informed consent to
participation to the study. We shall administer consent at two levels; first we shall
administer and obtain consent for participation in the study and thereafter consent for
collection and transportation of blood samples to Italy. The blood samples collected
will be used only for the purpose of this study and will not be stored beyond the
current study project. All unprocessed blood samples will be destroyed from the
department of infectious disease, university of Bari-Italy as biological sanitary waste,
at the end of the project. The same standard of care will be guaranteed to all pregnant
women irrespectively of participation. The dignity of study participants will be
guaranteed by the investigators, as well data confidentiality and the right to withdraw
data and/or biological samples at any time. Consent to pregnant women and to mature and
emancipated minors will be obtained according to Uganda Human Subjects Protection
Guidelines.
For all malaria positive samples processed for molecular analysis in the present study,
identifying information will be removed to provide appropriate protection of medical
confidentiality and privacy. In this way, sensitive data cannot be linked or re-linked
with identifiable human subjects, making anonymous each sample processed.
The polymorphisms analysis of the propeller domain of the Pfk13 gene will be performed
by advanced amplifications techniques and subsequent Sanger sequencing in a highly
specialized scientific laboratory. The blood samples collected in this study will be
sent to a laboratory in the University of Bari, Italy, in order to have access to the
latest Pfk13 gene amplification techniques. To enhance local capacity building one
laboratory staff from Aber hospital will be supported to attend a two weeks exposure at
the reference laboratory in Italy Clearance for blood sample shipment to Italy: Approval
for sample transportation will be obtained from UNCST before shipment of any blood
samples to Italy. A request for transfer of the blood samples will be made in writing to
the executive secretary of UNCST. This will be accompanied by a material transfer
agreement (MTA) between Pope John hospital Aber (the transferor), Doctors with Africa
CUAMM (the study partner), and the Department of Infectious Diseases-University of Bari
(the recipient) . The exact specification of the transfer conditions and the approved
study protocol will as well be submitted to UNCST with the request for the transfer of
the blood samples.
