In-vitro Fertilization Clinical Trial
Official title:
Time-lapse Evaluation of Human Embryo Development in Single Versus Sequential Culture Media - a Sibling Oocyte Study
Current human IVF technology employs two strategies with respective culture media for embryo development; 'let the embryo choose' (utilizes single step media) and 'back-to-nature' (utilizes sequential media). To our knowledge, there is not any sibling oocyte study to evaluate potential advantages/disadvantages of these strategies on one another.
Maintaining optimal embryo development during in vitro culture is a key element in assisted
reproduction treatments. Whether the concept of mimicking in vivo environment is a 'must' in
order not to compromise outcome is a matter of debate since 'let the embryo choose' strategy
has been shown to perform at least as good as the 'back to nature' in several studies.'Let
the embryo choose" approach utilizes a milieu with relatively constant concentration of
ingredients (single culture system) as compared to the latter, in which demands of the
embryos are supported according to their passage from the Fallopian tubes to the uterus in a
sequential culture media. Basic differences between these ingredients are; presence of (1)
glucose instead of pyruvate, and (2) essential aminoacids in early embryo stages (until day
3) in single step media, and (3) presence of EDTA in sequential media.
Vast majority of these results were obtained from studies where patients have been
randomized into groups instead of employing a sibling (oocyte/embryo) model which could have
enabled each patient to serve as her own control. In fact, it has been claimed that
introducing a sibling oocyte study design could give a methodological problem at the time of
fertilization since different type of culture media vary in osmolarity. Likewise the only
study that employed a sibling oocyte model utilized two different media (home-made versus
G1.2/G1.2) although the osmolarity of the former had been adjusted to 280-285 mOsm). This
study reported no significant differences in day3/5 embryo development as well as
pregnancy/implantation rates between groups.
Subsequent studies, however, favoured single step media, as they found higher proportion of
compacted embryos to zygotes at day3, morula at 4, a higher blastocyst yield, an overall
higher utilization rate after day5 and an improved implantation rate when compared to
sequential media. Yet, besides potential variation of gamete qualities between patients
allocated to groups (as described above), differences in static observation times of embryos
between laboratories could have affected the results in these studies. Current introduction
of time-lapse equipment into human assisted reproduction technology enabled a more distinct
definition of embryo development and it is now possible to quantify pace of embryo cleavage
in various media. Such a definition is also useful to determine the predictive value of
embryo development in pre-defined time points will be applicable to all embryology
laboratories using various culture systems.
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Observational Model: Case Control, Time Perspective: Prospective
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