Hyperlipidemia, Periodontitis, Gingivitis Clinical Trial
Official title:
GINGIVAL CREVICULAR FLUID MALONDIALDEHYDE, PROTEIN CARBONYL AND TOTAL ANTIOXIDANT CAPACITY IN PATIENTS WITH PERIODONTAL DISEASE AND HYPERLIPIDEMIA
we hypothesized that hyperlipidemia as an unfavourable levels of lipoprotein subfractions have deleterious impact on the development of periodontal infection by altering oxidative stres status of periodontal tissues. The aim of this study was therefore to investigate i) effect of hyperlipidemia on oxidative change in GCF content, ie. MDA, PC and TAOC levels, in patients with different periodontal status,
An observational study was performed in 45 hyperlipidemic(22 females, 23 males) and 45 age
and sex matched normolipidemic (25 females, 20 males) healhy controls. The participants were
recruited as a joint collaboration between Periodontology Department of the Faculty of
Dentistry and the Endocrinology and Metabolic Diseases Department of the Faculty of Medicine
at Ondokuz Mayis University in Samsun, Turkey between january 2013 and august 2014.The study
protocol was approved by the Local Ethics Committee, and written informed consent was
obtained from all study participants in accordance with the Helsinki Declaration (revised in
2000) It has been asserted that elevated serum lipid levels create a pro-inflammatory state,
which leads to an increase in oxidative state by composing an imbalanced production between
highly reactive molecular species and antioxidant defences, consequently predisposing one to
infections. Hyperlipidemia claimed to lead an increase in production of reactive oxygen
species (ROS) and lipid peroxidation (LPO). On the other hand it has been suggested that
high-cholesterol diet increases OS and causes oxidative damage in various organs. Also, OS
related mediators have frequently shown to be associated with chronic periodontitis (CP)
related inflammatory responses . Excessive ROS derived radical formations reported to have
an important role in the inflammatory process by leading to damage to proteins, DNA,
carbohydrates, and lipids.
Hyperlipidemia was defined as the presence of one or more altered values of the lipid
profile and the following cut-off values were used according to the laboratory's
recommendation: TC>200mg/dl; TG>200mg/dl; LDL cholesterol >130 mg/dl; HDL <35mg/dl).
Periodontal status was determined by evaluating the following clinical parameters: Silness &
Löe plaque index ; Löe & Silness gingival index ; Probing pocket dept,clinical attachment
level, bleeding on probing (BOP) measurements were performed on 6 sites per tooth
(mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual) using a
Williams periodontal probe. GCF collection was subsequently performed using those sites that
fit the criteria for GCF sampling described below.
All samples were collected between 8-10 am on the day following periodontal status
assessment. Samples were collected from the deepest 6 sites in the chronic periodontitis
group. In the gingivitis group samples were collected from the teeth with bleeding on
probing, whereas teeth without BOP were chosen in the healthy group. GCF samples were
collected from the similar 6 sites in the gingivitis and periodontally healthy groups in
order to maintain consistency of sampling. Accordingly, total of 90 GCF samples were taken
from each of the 6 groups (15 individuals per group x 6 sites).
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Observational Model: Case Control, Time Perspective: Prospective