Combined Effect of Exercise Intensity With Nutritional Supplementation on Skeletal Muscle Function

Healthy Volunteers Clinical Trial

— NUTSPORT  

Official title:

Combined Effect of Exercise Intensity With Nutritional Supplementation on Skeletal Muscle Function

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05350566
• Other study ID #: 2021-00373
• Status: Completed
• Phase: N/A
• Start date: June 7, 2022
• Est. completion date: December 7, 2022

Study information

• Verified date: May 2023
• Source: University of Lausanne
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The main objective of this study is to determine the effectiveness of a nutritional intervention (olive leaf extract) combined with a specific training session (MICT or SIT) on PDH activation (as well as other protein expressions) and exercise-induced muscle fatigue.

Description:

Sprint interval training (SIT) is a form of interval training characterised by short bursts of intense exercise interspersed with rest periods. SIT is as, or more, effective than moderate intensity continuous training (MICT) for systemic and metabolic adaptations of muscles, despite a lower workload and shorter duration. However, the mechanisms underlying the metabolic response to SIT versus MICT are poorly understood. Our recent work (Zanou et al. 2021) suggests that increased pyruvate dehydrogenase (PDH) activity may explain why a SIT session led to greater changes in proteins involved in mitochondrial respiration compared to a MICT session.

Recently, it was found that a natural and commercially available compound based on olive leaf extract could stimulate resting mitochondrial bioenergetics by activating the mitochondrial calcium uniporter (MCU), which is involved in calcium transport across the mitochondrial membrane, directly controlling PDH activity. The main objective of this study is to determine the effectiveness of an acute nutritional intervention (olive leaf extract) combined with a specific training session (MICT or SIT) on PDH activation (as well as other protein expressions) and exercise-induced muscle fatigue.

Participants will be allocated to either a SIT (6 x 30 s all out sprints) or a MICT (1h at 50% maximal aerobic power) group and asked to come to the laboratory for five visits including one familiarization session with a triangular test for maximal aerobic power determination, the first experimental session during which they will realize the exercise with the product or a placebo and a session 24h after to assess skeletal muscle adaptations. Then after a 3 week wash-out period, they report to the laboratory for the fourth and fifth session, identical to the second and third sessions but with the other intervention (product or placebo). Muscle microbiopsies will be collected from the vastus lateralis muscle before, immediately after and 24 hours after exercise (MICT or SIT), while knee extensor neuromuscular function will be assessed at the same time points using voluntary and electrically-evoked contractions.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 25
• Est. completion date: December 7, 2022
• Est. primary completion date: December 7, 2022
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: Male
• Age group: 18 Years to 40 Years

Eligibility

Inclusion Criteria:

1. Being male (including females would necessitate increasing the number of included participants to consider sex as a variable which would burden the project too much).

2. Being aged between 18 and 40 years

3. Being healthy (The assessment will be done through a questionnaire and an interview/examination by a medical doctor if there is any doubts)

4. Being physically active, but not involved in any structured training program

5. Having stable dietary habits

Exclusion Criteria:

1. Having previous medical events that would put the participant at risk during the study

2. Ingesting caffeine on the experimental days

3. Not being currently treated for blood clotting problems

4. Taking aspirin or anti-coagulant drugs in the 48 hours preceding the experimental days in which muscle samples will be collected

5. Having allergy to xylocaine

6. Having food allergy

Related Conditions & MeSH terms

• Fatigue
• Healthy Volunteers
• Neuromuscular Fatigue

Intervention

Dietary Supplement:
• Olive leaf extract
A capsule containing 250 mg of an olive leaf extract preparation will be swallowed with a glass of water before exercise.
• Placebo
A capsule containing 336 mg of cellulose microcrystalline will be swallowed with a glass of water before exercise.

Locations

Country	Name	City	State
Switzerland	Bâtiment Synathlon, quartier UNIL-Centre	Lausanne

Sponsors (2)

Lead Sponsor	Collaborator
University of Lausanne	Société des Produits Nestlé (SPN)

Country where clinical trial is conducted

Switzerland, 

References & Publications (2)

Place N, Ivarsson N, Venckunas T, Neyroud D, Brazaitis M, Cheng AJ, Ochala J, Kamandulis S, Girard S, Volungevicius G, Pauzas H, Mekideche A, Kayser B, Martinez-Redondo V, Ruas JL, Bruton J, Truffert A, Lanner JT, Skurvydas A, Westerblad H. Ryanodine receptor fragmentation and sarcoplasmic reticulum Ca2+ leak after one session of high-intensity interval exercise. Proc Natl Acad Sci U S A. 2015 Dec 15;112(50):15492-7. doi: 10.1073/pnas.1507176112. Epub 2015 Nov 2. — View Citation

Zanou N, Dridi H, Reiken S, Imamura de Lima T, Donnelly C, De Marchi U, Ferrini M, Vidal J, Sittenfeld L, Feige JN, Garcia-Roves PM, Lopez-Mejia IC, Marks AR, Auwerx J, Kayser B, Place N. Acute RyR1 Ca2+ leak enhances NADH-linked mitochondrial respiratory capacity. Nat Commun. 2021 Dec 10;12(1):7219. doi: 10.1038/s41467-021-27422-1. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	PDH dephosphorylation	protein involved in skeletal muscle metabolism	before, immediately after and 24 hours after exercise completion
Primary	expression of OXPHOS proteins	proteins used as markers of oxidative phosphorylation complexes	before, immediately after and 24 hours after exercise completion
Primary	expression of MCU protein	protein involved in the transport of Ca2+ in the mitochondria	before, immediately after and 24 hours after exercise completion
Secondary	Maximal voluntary contraction force	Maximal force of the knee extensor muscles	before, immediately after and 24 hours after exercise completion
Secondary	Voluntary activation level	overall index of central fatigue induced by exercise	before, immediately after and 24 hours after exercise completion
Secondary	Peak doublet evoked at 100 Hz	overall index of peripheral fatigue induced by exercise	before, immediately after and 24 hours after exercise completion
Secondary	M-wave amplitude	index of neuromuscular excitability	before, immediately after and 24 hours after exercise completion