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Gene Product Sequence Variation clinical trials

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NCT ID: NCT06075979 Recruiting - Clinical trials for Gene Product Sequence Variation

Correlation Between Microbial Infection and Lumbar Degenerative Disease Based on High-throughput Gene Sequencing

Start date: April 2, 2024
Phase: N/A
Study type: Interventional

Lumbar degenerative disease is one of the most common diseases in orthopedic and spinal surgery. The pathogenesis of lumbar degenerative disease is still unclear, mainly including aging degeneration and biomechanical hypothesis. In our previous research work,the investigators took lumbar disc tissue from patients who underthe investigatorsnt surgical treatment for lumbar degenerative diseases. The investigators found that some patients had low-toxic bacterial infection in the intervertebral disc tissue. Combined with literature and previous studies, it is suggested that microbial infection plays a role in lumbar degenerative diseases. The investigators suggest that microbial infection may be closely related to the occurrence and development of lumbar degenerative diseases, which may cause or even accelerate the degeneration of lumbar intervertebral disc tissue. The current research difficulties are as follows: 1. Low sensitivity and specificity of microbial analysis; 2. It is difficult to distinguish the colonization infection of intervertebral disc tissue microorganisms from the contamination of foreign substances. In view of this, this study intends to use the high-throughput gene sequencing technology of infectious pathogens based on nano single molecule sequencing, double verification of blood samples and intervertebral disc tissue samples, to identify the microbial status of degenerative lumbar disc tissue, and to explore the correlation between lumbar degenerative disease and microbial infection, identifying relevant susceptible microorganisms, which is expected to study the pathogenesis of this susceptible microorganism in the future, and provide new ideas and approaches for the prevention, control and treatment of lumbar degenerative diseases.

NCT ID: NCT03192501 Recruiting - Cancer Clinical Trials

iCAGES-guided Precision Therapy for Cancers in Contrast to Standard Care or IHC-guided Theray

Start date: July 1, 2017
Phase:
Study type: Observational

This study prospectively evaluates whether the use of iCAGES (integrated CAncer GEnome Score) tool in guiding the treatment of advanced cancers is superior to current standard care or IHC-guided therapy in progress free survival (PFS),overall survival (OS),and improvement of life quality.

NCT ID: NCT03043209 Completed - Clinical trials for Hypertrophic Cardiomyopathy

Genomic Sequencing in Patients With HCM Undergoing Septal Myectomy

Start date: August 30, 2018
Phase:
Study type: Observational

Investigators aim to use comparative exome and/or genome sequencing to discover causative molecular lesions for phenotypes hypothesized to be caused by somatic mutations. For this study, investigators have targeted hypertrophic cardiomyopathy.

NCT ID: NCT03018574 Completed - Clinical trials for Gene Product Sequence Variation

Candidate Gene Screening for Attention Deficit/Hyperactive Disorder (ADHD)

Start date: May 2016
Phase: N/A
Study type: Observational

Source: Sample bank of Xijing Hospital and Children's Hospital Affiliated to Soochow University; Sample form: Whole blood; Estimated number of samples: 100 patients with ADHD and age, sex matched healthy controls; Case exclusion criteria: all kinds of neuropsychiatric disorders, IQ value of less than 70; Study protocol: 1. Using Qiagen kit to extract the genomic DNA of 200 microliters of blood. 2. UV spectrophotometer test DNA purity of 260/280 close to 1.8 (1.8 ± 0.05), the concentration of 100ng/μL or more before the next sequencing. 3. The extracted genomic DNA will be sent to Sangon Biology Engineering Limited Company (Shanghai) and sequenced to find candidate mutations related to ADHD risk sequence. According to NIH gene database, the longest transcript of NDRG2 (ID: 57447 gene, https://www.ncbi.nlm.nih.gov/nuccore/NC_000014.9? Report=genbank&from=21016763&to=21070872&strand=true) (a total of 17 exons and 16 introns and the gene 5 'UTR and 3' UTR region) will be alignmented sequences to find potential mutations. 4. Using the chi square analysis and other statistical methods to determine the relationship between the mutations and susceptibility to ADHD.