Gastroesophageal Reflux Disease With Esophagitis Clinical Trial
Official title:
Evaluate the Intestinal Microbiome in Patients With Erosive Gastroesophageal Reflux Disease and in Asymptomatic Healthy Controls
. In this study, we investigated intestinal microbiomes, in individuals with Gastroesophageal Reflux Disease and healthy individuals, using metagenomic techniques.
Background: The pathophysiology of Gastroesophageal Reflux Disease (GERD) involves several
mechanisms. Intestinal dysbiosis may influence factors involved in the pathophysiology of
Gastroesophageal Reflux Disease, such as changes in gastrointestinal motor patterns and
increased intraabdominal pressure due to increased fermentation of non-digestible
carbohydrates. However, few studies have focused on assessment of gut microbiome in the GERD.
In this study, we investigated intestinal microbiomes, in individuals with Gastroesophageal
Reflux Disease and healthy individuals, using metagenomic techniques.
Methods: We performed a study comprising fecal samples of 22 adults, aged 18-60 years: 11
with erosive esophagitis (eight male and three female) and 11 healthy controls (ten male and
one female). Microbiome DNA was extracted from stool samples, and polymerase chain reaction
amplification was performed using primers for the V4 region of the 16S rRNA gene (16S
ribosomal ribonucleic acid). The amplicons were sequenced using the Ion Torrent™ Personal
Genome Machine™ platform, and data were analyzed using QIIME™ software version 1.8
(Quantitative Insights Into Microbial Ecology).
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