Enterovirus 71 and Influenza Virus Clinical Trial
Official title:
Virosensor -Based Real-time Diagnosis of Enterovirus 71 and Influenza Virus
To develop a real-time diagnostic technique with Virosensor for Enterovirus 71 and Influenza Virus detection, the investigators conduct a prospective clinical study. In comparison with results from direct sequencing of Enterovirus 71 and Influenza Virus, the investigators evaluate the performance of virosensor , including reproducibility, sensitivity, specificity, and cross-reaction. The potential factors which may interfere with the results would be investigated. With such technique, the investigators hope to make early diagnosis and give Enterovirus 71 and Influenza Virus patients early treatment to reduce the complications and case-fatality rate.
1. Sample acquirement： At National Taiwan University Hospital in Taiwan, we will enroll
patients who have influenza-like illness or enterovirus infection . We will take three
throat swabs for each patient: one for viral isolation, one for RT-PCR and the last for
the Virosensor - based real-time diagnosis.
2. Virosensor diagnosis： Virosensor (structural chip-based optosensing virus probing
system), which is for the rapid and sensitive detection of viral antigen in medical
samples, will be used for analyzing the interaction kinetics between
anti-influenza-virus (or anti-Enterovirus 71-virus) and its influenza virus antigen (or
Enterovirus 71 antigen) present in patients'and normal samples.
3. Gold standard： viral isolation and RT-PCR
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Intervention Model: Single Group Assignment, Masking: Double Blind (Investigator, Outcomes Assessor), Primary Purpose: Diagnostic