Analysis of Bacterial Microbiome of Endodontically Infected Primary and Permanent Teeth

Endodontic Disease Clinical Trial

Official title:

Mersin University Clinical Research Ethics Committee

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04211519
• Other study ID #: 2017/234
• Status: Completed
• Phase: N/A
• Start date: September 1, 2017
• Est. completion date: December 10, 2019

Study information

• Verified date: December 2019
• Source: Nuh Naci Yazgan University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Recognition of community profiles in endodontic infections may allow a better understanding of the pathogenesis of the disease and the establishment of more effective treatment protocols.

Therefore, the aim of the present study was to investigate bacterial diversity in endodontically infected primary and permanent teeth using 16S rRNA gene sequencing and QIIME 2TM (Quantitative Insights Into Microbial Ecology 2) bioinformatics pipeline

Description:

Endodontic infections are defined as an infection of the pulp and periapical tissues. This infection is caused by microorganisms that invade the pulp via dental caries or dental trauma. In these mixed population infections, anaerobic bacteria have been reported to be conspicuously dominant and the number of microorganisms per canal may vary. Traditionally, the endodontic microbiome has been identified by culture-based (phenotype-based) techniques. Inability to cultivate approximately 40-55% of bacteria in the endodontic microbiome, bias or inexperience of researchers may limit the results of cultural research. Microbiome-based new generation sequencing (NGS), which was initially used in ecological studies, has been widely used in recent years to identify bacterial diversity using the 16S ribosomal RNA (rRNA) gene in endodontic infections microbiome, bias or inexperience of researchers may limit the results of cultural research. There are few studies investigating the endodontic microbiome in primary teeth. However, to the best of our knowledge, to date, there is no metagenomic study that investigates the endodontic microbiome of the primary and permanent teeth. It is a still question of whether there is a difference in the endodontic microbiomes during the mixed dentition period when both dentition types can be seen.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 30
• Est. completion date: December 10, 2019
• Est. primary completion date: February 15, 2018
• Accepts healthy volunteers: No
• Gender: All
• Age group: 4 Years to 13 Years

Eligibility

Inclusion Criteria:

• have intact roots or <1/3 of physiological root resorption

• have clinical crowns that permit effective rubber dam isolation

• no mobility, fistula, pus discharge, gingival swelling, periapical abscess or internal resorption.

Exclusion Criteria:

• have marginal periodontitis, a history of pharmacological treatment, antibiotics or fluoride intake within the last 2 months

• a history of cancer, diabetes or immunodeficiency disorders

Related Conditions & MeSH terms

• Dental Pulp Diseases
• Endodontic Disease
• Endodontic Inflammation
• Inflammation
• Pulpitis

Intervention

Diagnostic Test:
• sampling
sampling from endodontically infected primary and permanent teeth by using paper point method

Locations

Country	Name	City	State
Turkey	Nuh Naci Yazgan Üniversitesi	Kayseri	Kocasinan

Sponsors (2)

Lead Sponsor	Collaborator
Nuh Naci Yazgan University	Mersin University

Country where clinical trial is conducted

Turkey, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Comparison of microbiome composition of endodontically infected primary and permanent tooth canal	Analysis of the profile of microbial populations based on 16S rRNA gene analysis in endodontically infected permanent and primary teeth	6 months