DNA Methylation Clinical Trial
Official title:
Cumulative Live Birth Rate With eSET After Preimplantation DNA Methylation Test (PIMT) in ART
NCT number | NCT03642574 |
Other study ID # | PIMT |
Secondary ID | |
Status | Completed |
Phase | N/A |
First received | |
Last updated | |
Start date | September 5, 2018 |
Est. completion date | June 30, 2022 |
Verified date | October 2022 |
Source | Shandong University |
Contact | n/a |
Is FDA regulated | No |
Health authority | |
Study type | Interventional |
The purpose of this clinical trial is to determine the safety and effect of methylation level of DNA methylation in embryos on the outcome of assisted reproductive technology (ART) during blastocyst embryo screening. Subjects with blastocysts on day 5-7 of embryo culture will be biopsied. A Freeze-all strategy and a single frozen blastocyst transfer will be performed till all study-specific embryos have been transferred. Then whole genome bisulfate sequencing will be performed on all cells that obtained from biopsy.
Status | Completed |
Enrollment | 182 |
Est. completion date | June 30, 2022 |
Est. primary completion date | August 28, 2021 |
Accepts healthy volunteers | No |
Gender | Female |
Age group | 20 Years and older |
Eligibility | Inclusion Criteria: 1. Women who are participating in preimplantation screening with PGS indications,defined as maternal age above 38 years, repeated implantation failure (RIF) usually defined as three or more transfers of morphologically high-quality embryos without the establishment of pregnancy, recurrent miscarriage (RM) in patients with normal karyotypes (usually at least three previous consecutive miscarriages) and severe male factor infertility (usually defined as abnormal semen parameters). 2. Women who obtain 2 or more good-quality blastocysts that defined as morphological score of inner cell mass B or A, trophectoderm C or better, and grade 4 or better on Day five of embryo culture will be randomized. Exclusion Criteria: 1. Women with a uterine cavity abnormality, such as a uterine congenital malformation (uterus unicornate, bicornate, or duplex); untreated uterine septum, adenomyosis, submucous myoma, or endometrial polyp(s); or with history of intrauterine adhesions. 2. Women with untreated hydrosalpinx. 3. Women who use donated oocytes or sperm to achieve pregnancy. 4. Women with contraindication for assisted reproductive technology or for pregnancy, such as poorly controlled Type I or Type II diabetes; undiagnosed liver disease or dysfunction (based on serum liver enzyme testing); renal disease or abnormal serum renal function; significant anemia; history of deep venous thrombosis, pulmonary embolus, or cerebrovascular accident; uncontrolled hypertension, known symptomatic heart disease; history of or suspected cervical carcinoma, endometrial carcinoma, or breast carcinoma; undiagnosed vaginal bleeding. |
Country | Name | City | State |
---|---|---|---|
China | Shandong University | Jinan | Shandong |
Lead Sponsor | Collaborator |
---|---|
Chen Zi-Jiang | Chinese Academy of Sciences |
China,
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* Note: There are 12 references in all — Click here to view all references
Type | Measure | Description | Time frame | Safety issue |
---|---|---|---|---|
Other | Clinical pregnancy rate after the first transfer | Number of women with clinical pregnancies after the first transfer / number of women. | 4 months | |
Other | Pregnancy loss rate after the first transfer | Number of pregnancy losses / number of clinical pregnancies after the first transfer . | 9 months | |
Other | Live birth rate after the first transfer | Number of women with live births after the first transfer / number of women. | 12 months | |
Primary | The effect of DNA methylation level on live birth rate | The rate of live birth at different methylation level will be calculated. Live birth is defined as the delivery of any viable infant at 28 weeks or more of gestation, and cumulative live birth rate is calculated by dividing the number of women achieving live birth after transfers (up to 3 transfers of single blastocycst within 1 year). | 22 months | |
Secondary | The effect of DNA methylation level on pregnant rate, pregnant loss rate. | The rate of pregnant and pregnant loss at different methylation level will be calculated. Pregnancy loss refers to a complete spontaneous abortion or a nonviable pregnancy before 28 weeks of gestation. | 22 months | |
Secondary | Duration of pregnancy | The time from the first day of last menstrual period to the day of delivery. | 22 months | |
Secondary | Birth weight | Weight of newborns at delivery. | 22 months | |
Secondary | Cumulative incidence of maternal complications during whole | Number of pregnancies with complications / number of pregnancies over (up to) 3 transfers within 1 year; | 22 months | |
Secondary | Cumulative incidence of neonatal complications during whole | Number of live births with neonatal complications / number of live births over (up to)3 transfers within 1 year | 22 months | |
Secondary | Number of embryo transfers to achieve live birth | Number of embryo transfers the patients have gone through to achieve live birth. | 22 months |
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