Resistance Exercise and Hydrolyzed Collagen Supplementation

Diet, Food, and Nutrition Clinical Trial

Official title:

The Dose-response of Vitamin C-enriched Collagen on Markers of Collagen Synthesis in Healthy Young and Older Men and Women Following Resistance Exercise

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05932771
• Other study ID #: 18/SPS/059
• Status: Completed
• Phase: N/A
• Start date: January 1, 2019
• Est. completion date: April 30, 2023

Study information

• Verified date: June 2023
• Source: Liverpool John Moores University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Description:

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Healthy young and older males and females ingested 0 grams, 15 grams or 30 grams HC with 50 milligrams vitamin C 1h prior to performing four sets' barbell back-squat RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of collagen breakdown) concentration, and the concentration of 18 amino acids that constitute collagen.

This is the first study to investigate the combined effect of different doses HC with high-intensity RE on whole body collagen synthesis in healthy young and older men and women. If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this suggests that long-term HC intake with chronic RE would be beneficial for tendon health, because collagen is the most abundant protein in tendon.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 21
• Est. completion date: April 30, 2023
• Est. primary completion date: August 30, 2019
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 65 Years

Eligibility

Inclusion Criteria:

• At least 12 months experienced in resistance exercise (regular back squat experience)

• Free from musculoskeletal injury

• Nullipara (a woman who has never given birth) (only for young female participatns)

Exclusion Criteria:

• Having a history of patellar tendon pathology

• Vegan

• Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)

• Had a sustained a lower limb injury in the previous six months

• Smoker/vaper

• <18 or >40 years old (only for young participants)

• <40 ro >65 years old (only for older participants)

• Having an irregular menstrual cycle (only for young female participants)

Related Conditions & MeSH terms

• Diet, Food, and Nutrition

Intervention

Dietary Supplement:
• Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise
Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All trials were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams, 15 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of nutritoinal supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)
• Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise
Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula. All trials were performed at the same time of day (08:00 - 15:00). Different doses of HC (0 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams maltodextrin was used in 0 grams HC. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses. The details of nutritoinal supplements used are as follows: Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

Locations

Country	Name	City	State
United Kingdom	Dr Rob Erskine	Liverpool

Sponsors (2)

Lead Sponsor	Collaborator
Rob Erskine	University of East Anglia

Country where clinical trial is conducted

United Kingdom, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in a marker of collagen synthesis	Serum procollagen type ? N-terminal propeptide (P?NP) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.	at rest immediately prior to HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE
Primary	Change in a marker of collagen breakdown	Plasma ß-isomerized C-terminal telopeptide of type I collagen (ß-CTX) concentrations were measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.	at rest immediately prior to HC ingestion and 6-hour post RE
Primary	Changes in amino acids concentrations in blood	Concentration of serum amino acids that constitute collagen was measured following 0 grams, 15 grams or 30 grams hydrolyzed collagen (HC) intake with resistnace exercise (RE) during all trials.	at rest immediately prior to HC ingestion, 0.5-hour post HC ingestion, 1-hour post HC ingestion, 0.5-hour post RE, 1-hour post RE, 2-hour post RE, 4-hour post RE and 6-hour post RE
Secondary	Estrogen concentrations in women in each trial	Concentrations of estrogen (17ß-estradiol) was measured in each trial.	at rest immediately prior to HC ingestion