Correlation of Cytokines and Anthropometry in Pregnancy Clinical Trial
Official title:
Cytokines Profile at the Beginning of Pregnancy in Mexican Women
A mechanism by which obesity could influence the increase in obstetric complications as more weight is gained, apart from oxidative stress (3) would be the changes in the expression profile of T helper (Th) cells. Due to the increase rate of obesity among Mexican women, the aim was to determine if there is some association between the weight changes in pregnancy and the cytokines Interleukin (IL)-10, IL-4, Interferon gamma (IFN-γ) and Tumor Necrosis Factor alpha (TNF-α).
Pregnant women older than 18 years old were invited to participate and all were asked to sign
the informed consent. Women with congenital heart disease, disabling diseases, infectious
diseases and autoimmune diseases were excluded and those whose clinical follow-up was lost
were discarded from the final analysis.
Weight and height were measured using a calibrated using an adult scale (Seca, Hamburg,
Germany). Blood Pressure (BP; mmHg) was checked by auscultation using a standard
sphygmomanometer (Riester Big Ben® Square; Jangingen, Germany). All mothers received written
nutritional guidance depending on the characteristics of each patient.
The expression levels of IL-10, IL-4, IFN-γ and TNF-α was performed by the ELISA technique.
Quantitative variables will be represented by measures of central tendency. Once the
Kolmogorov test is performed for the normality of the variables, either the Student's T test
or the Mann Whitney U test will be used to compare both groups. With the same criteria for
normality either Pearson or Spearmen correlation will be used among the cytokines and
anthropometric variables, considering a significant difference at p ≤ 0.05. The statistical
analysis will be carried out in the Statistical Package for the Social Sciences (SPSS)
program, version 19.
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