FOS Immunohistochemical Staining of Colorectal Cancer and Its Adjacent Tissues

Colorectal Cancer Clinical Trial

Official title:

Monkeypox and Cancer: a Pan-cancer Based Multi-omics Analysis and Single Cell Sequencing Analysis-Experimental Studies

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT05698082
• Other study ID #: KY-2020-305
• Status: Recruiting
• Phase: Phase 2/Phase 3
• Start date: December 1, 2020
• Est. completion date: January 1, 2025

Study information

• Verified date: January 2023
• Source: Cancer Hospital of Guangxi Medical University
• Contact: Xiaoliang Huang, Dr.
• Phone: 0086-13617713823
• Email: xiaoliang[@]sr.gxmu.edu.cn
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Colorectal cancer tissue sections were obtained according to the inclusion criteria. The formalin was used to immersed all cancer specimens. And tissues were cut to 5 μm thickness and placed on glass slides before staining. Endogenous peroxidase activity was inhibited and blocked by de-paraffinizing, rehydrating, and using 5% bovine serum albumin at 37ºC for 30 min. The treated sections were incubated with anti-FOS (promab 30360) at 4ºC overnight and washed three times with PBS. After that, it is required that incubation with secondary anti-peroxidation sunflower at 37ºC for 30 minutes. After washing three times again with PBS, the sections were developed in diaminobenzidine and microscopic images were made by light microscopy.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 120
• Est. completion date: January 1, 2025
• Est. primary completion date: January 1, 2025
• Accepts healthy volunteers: No
• Gender: Male
• Age group: 18 Years to 75 Years

Eligibility

Inclusion Criteria:

• (a) patients with histologically confirmed CRC (b) patients who underwent resection of the primary tumor (c) patients at first visit who had not received treatment before blood testing.

Exclusion Criteria:

• (a) familial adenomatous polyposis or hereditary colon cancer (b) no signs of clinical infection, such as fever, on the day of blood collection; And (c) patients with multiple primary tumors during hospitalization.

Related Conditions & MeSH terms

• Colorectal Cancer
• Colorectal Neoplasms
• Monkey Pox
• Monkeypox

Intervention

Diagnostic Test:
• Immunohistochemical staining with anti-FOS
The tissue sections were incubated with anti-FOS (promab 30360) at 4ºC overnight and washed three times with PBS. After that, it is required that incubation with secondary anti-peroxidation sunflower at 37ºC for 30 minutes. After washing three times again with PBS, the sections were developed in diaminobenzidine and microscopic images were made by light microscopy.

Locations

Country	Name	City	State
China	Guangxi Medical University Cancer Hospital	Nanning	Guangxi

Sponsors (1)

Lead Sponsor	Collaborator
xiaoliang Huang

Country where clinical trial is conducted

China, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Original survival	Time from the start of the study to death from any cause. For subjects who were lost to follow-up before death, the time of last follow-up was calculated as the time of death.	Participants were followed up for 3 years, including outpatient follow-up and telephone follow-up.