Clinical Trials Logo

Clinical Trial Summary

The aim of this study is to evaluate the comparison of therapeutic potential of curcumin preconditioned adipose derived stem cells (ASCs) enrichment fat grafting, naïve ASCs enrichment fat grafting and conventional fat grafting to correct facial contour deformities that cause aesthetic complications in patients.


Clinical Trial Description

Human subcutaneous adipose tissue along informed consents will be collected in a lipoaspirate container or 20 cc B.D syringes under the sterilized conditions during the surgery. Fat aspirate collection will be based on certain selection criteria including pre-requisitioning non-diabetic, negative viral status patients. Adipose derived stem cells (ASCs) will be isolated from adipose tissue under sterile aseptic conditions. Firstly, the lipoaspirate container will be opened in the biosafety cabinet and drain off the blood carefully with the help of serological pipette. Then, fat will be washed thrice with 1X phosphate buffer saline (PBS) containing antibiotic and anti-mycotic solution, in order to get rid of blood vessels, hair and other type of connective tissue. After that, the tissue will be enzymatically digested with collagenase type I solution (1mg/ml prepared in Low Glucose Dulbecco's Modified Eagle Medium (LG-DMEM) and incubate for 45 minutes at 37°C on a shaker. After digestion, the enzyme will be inactivated by LG-DMEM augmented with 5% freshly isolated human serum and spin at 1200 rpm for 10 minutes. After centrifugation, fat will be discarded and the infranatant will be passed through 100 µm cell strainers to remove the debris. The filtrate will be centrifuged again for 1200rpm for 8 minutes. Pellet will be resuspended and plated in a sterile T-75 cm2 flask containing 10 ml LG-DMEM supplemented with 5% human serum. The flask will be placed in a humidified incubator at 37°C, 5% carbon dioxide (CO2). The media will be replenished after every third day until the cells got 80-90 % confluency. Cells of P2-P3 stage will be used in this study for immunocytochemistry, and intervention as well. For preconditioning, ASCs will be incubated with curcumin preconditioned medium for 24 h. Cell sterility and viability will be assessed prior to transplantation. For transplantation, 1,00,000 cells per ml of fat will be mixed, transferred to 10 cc syringes and injected at deformity site. Amount of fat will be predetermined by the clinician on the basis of facial asymmetry. ;


Study Design


Related Conditions & MeSH terms


NCT number NCT05610878
Study type Interventional
Source University of the Punjab
Contact Azra Mehmood, PhD
Phone +92-333-4107008
Email azra_mehmood@hotmail.com
Status Recruiting
Phase Phase 1
Start date November 20, 2020
Completion date December 2023

See also
  Status Clinical Trial Phase
Completed NCT03664505 - Evaluation of the Product Properties of Individualized 3D-compression Garments to Treat Scars N/A
Recruiting NCT05309720 - Dermal Substitution in Pediatric Burns N/A
Not yet recruiting NCT04995302 - Microneedling Therapy With or Without Amnion Bilayer Sheeting on Scar Tissue N/A
Not yet recruiting NCT04557514 - Comparative Study Between Fat Injection And Platelet Rich Plasma In Post Burn Facial Scar N/A
Recruiting NCT05555602 - Observation of the Comfort and Efficacy of CO2 Laser Combined With Cryo 6 for Burn or Linear Scars. N/A
Recruiting NCT01619917 - The Role of Fractional Vascular Laser Therapy in the Management of Burn Scars N/A
Recruiting NCT01488240 - The Role of Pulsed Dye Laser Therapy in the Management of Burn Scars N/A
Completed NCT03197649 - CO2 Laser Phototherapy for Management of Mature Burn Scars N/A
Completed NCT04619589 - Characterization of Dyschromic Hypertrophic Scar
Completed NCT03627650 - Fat Grafting in Skin-grafted Deep Burn Scars N/A
Completed NCT02417779 - Cutaneous Microcirculation After Extracorporeal Shock Wave Therapy N/A
Completed NCT02417818 - Cutaneous Microcirculation After Plasma Therapy N/A
Withdrawn NCT01075165 - Efficacy of Spray Silicone in Alteration of Burn Scar N/A
Recruiting NCT04364217 - Pain and Itch Reduction in Burn Scars Treated With Fractional CO2 Laser N/A
Completed NCT04412759 - PORCINE XENOGRAFT OR MICROBIAL CELLULOSE IN THE TREATMENT OF PARTIAL THICKNESS BURNS N/A
Recruiting NCT06122090 - Treatment of Hypopigmented Scars With Bimatoprost Phase 2
Recruiting NCT05423613 - Microneedling for Burn Hypertrophic Scars N/A
Active, not recruiting NCT04456127 - CO2 Laser Revision for Burn Related Donor Site Scars N/A
Completed NCT03544697 - Easy Way of Choosing Rectangular Expander and Getting Maximum Benefit From Expanded Tissue
Terminated NCT01564407 - A Clinical Study of Allogeneic Human Dermal Fibroblasts for Remodeling Scar Contractures Phase 1/Phase 2