Biomarkers of Food Intake in Healthy Subjects Clinical Trial
Official title:
Biomarkers of Meats and Potatoes Intake: a Meal Study in Healthy Men and Women - the MEPO Study.
The discovery of biomarkers for the intake of meats and potatoes is needed for an accurate assessment of the intake of these foods. Twelve healthy subjects were enrolled in a controlled, cross-over meal study, randomized by a Latin square design. The test meals contained 1) beef, pork, chicken and a control meal for the meats and 2) french fries, boiled potatoes, chips and a control for the potato meals. A standardized diet was provided during sample collection. Blood and urine samples were collected up to 48h primarily for untargeted metabolomic profiling. Blood was further collected to study the effect of the meals on insulin and glycemic responses. Effects on satiety were measured by VAS and an ad libitum lunch following the test meals.
Protein-rich diets improve body weight regulations and are thus believed to play a key role
in combating the global obesity epidemic. Protein-rich diets are generally high in meats,
some of which have become controverted enough to be considered disease-promoting foods
despite their nutritional richness. The degree at which associations with disease differs
from causality is however not entirely known.
Assessment of food intake currently relies on food frequency questionnaires (FFQ), 24h recall
and dietary records, all of which are self-reporting methods known to be subject to bias and
inaccuracy. Biomarkers of food intake emerged thus within the past years to complement the
conventional tools as objective measurements of food intake, drawn from biological samples.
Several biomarkers of meat consumption have been proposed, however none is currently accepted
and used as biomarker(s) of meat intake and none is able to differentiate between the
different types of meat.
In the modern diet, meats are very often served with potatoes. To our knowledge, biomarkers
of potato intake have not been thoroughly reported in the literature. Different cooking
methods were shown to potentially influence the content of bioactive constituents of
vegetables, their glycemic index, as well as subsequent energy intake. High heat cooking
additionally yields the formation of potentially toxic compounds such as advanced glycation
endproducts (AGEs) that were shown to contribute to insulin resistance and type 2 diabetes.
In this line, biomarkers of potatoes as such as well as biomarkers to differentiate between
different cooking methods would be of great importance in advancing the understanding of
nutritional science.
Primary objectives:
1. To identify acute biomarkers of general meat intake by untargeted metabolomics
2. To explore the changes in the metabolome as a result of acute exposure to three types of
meat and identify meat-specific biomarkers of intake
3. To identify biomarkers of acute potato intake by untargeted metabolomics
4. To identify biomarkers that can differentiate between three types of cooking methods
5. To investigate the effect of the interaction between meat on potatoes on satiety,
assessed by registration of intake from an ad libitum meal
Secondary objectives:
1. To validate already known biomarkers of meat intake by targeted metabolomics
2. To investigate the effect of the interaction between meat and potatoes on satiety,
assessed by visual analogue scales
3. To investigate the effect of the interaction between meat and potatoes on glucose and
insulin responses
;