| Eligibility |
Inclusion Criteria:
1. Subjects who were able to understand and follow instructions during the study.
2. Subjects who signed informed consent.
3. Male subjects =18 and =55 years of age; female subjects =18 and =50 years of age of
non-childbearing potential defined as follows:
- Female subjects 50 years of age, in menopause for 24 consecutive months and not
receiving any hormone replacement therapy within 24 months prior to inclusion
into the study
- Female subjects who underwent surgical sterilization
- Female subjects who underwent hysterectomy
- Female subjects with documented premature ovarian failure
4. Weight within a BMI range of 19.0-30.0 kg/m2.
5. Estimated glomerular filtration rate (eGFR) according to the CKD-EPI: =90 mL/min
(normal renal function)
6. Healthy subjects have to be in a good health in the opinion of the study physician, as
determined by medical history, ECG, vital signs, physical examination, and clinical
laboratory tests.
7. Having had no febrile or infectious illness for at least 14 days prior to dosing.
8. The subject was available to complete the study.
9. The subject is willing to comply with the restrictions and requirements of the
protocol and, in the opinion of the study physician, will be able to complete the
study.
Exclusion Criteria:
1. Unwilling or unable to give informed consent.
2. As a result of the medical screening process, the study physician considered the
subject unfit for the study.
3. Pregnant or lactating women or men with female partners who are lactating or are
pregnant.
4. Known or suspected history of hypersensitivity to rifabutin or excipients or to drugs
of a similar chemical class including rifampicin, rifapentine, rifaximin; history of
allergic reactions leading to hospitalisation or any other allergic conditions
(including drug allergies, asthma, eczema, anaphylactic reactions but excluding
untreated, asymptomatic, seasonal allergies) which the Investigator considers may
affect the safety of the subject and/or out-come of the study.
5. History of antibiotic associated diarrhoea within the last year.
6. Subjects with ECG abnormalities (history, or evidence of second-degree heart block of
Mobitz type II, third degree heart block, or any abnormality considered relevant by
the Investigator), QTcF > 450 ms, PR > 210 ms, or QRS duration > 115 ms.
7. Average supine systolic blood pressure > 140 mmHg or < 90 mmHg or average diastolic
blood pressure > 90 mmHg or < 50 mmHg at Screening or Day 1 prior to dosing (any
abnormal blood pressure results may be repeated once and if the repeat result is
within the normal range, it is not considered to have met the exclusion criterion).
Pulse rate > 90 or < 50 beats per minute at Screening or Day 1 prior to dosing.
8. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase
(ALP) and creatinine, above the ULN at Screening. Any abnormal value of these
parameters may be repeated during screening period and if the repeat result is within
the laboratory reference range, it is not considered to have met the exclusion
criterion.
9. Screening values other than AST, ALT, ALP, creatinine, for haematology, biochemistry,
or urinalysis must not exceed the reference range. Minor deviations from normal are
allowed if they are not clinically significant.
10. History of symptomatic, chronic or recurrent infection (e.g. nausea, vomit-ing,
diarrhoea, infection with fever) or any viral (including symptomatic herpes zoster),
bacterial (including upper respiratory infection), fungal (non-cutaneous) or parasitic
infection within 30 days prior to admission to the clinical unit.
11. A positive pre-study serology test for hepatitis B surface antigen (HBsAg), hepatitis
C virus (HCV) antibodies or human immunodeficiency virus (HIV)-1 and/or 2 antibodies.
12. Positive Coronavirus (SARS-CoV-2) rapid test and/or PCR (upon Check-in on Day -1). If
a subject has negative rapid test but a positive PCR, the subject could be included if
his/her medical history is consistent with pre-vious COVID-19 infection explaining
his/her positive PCR within the last 90 days.
13. A positive pre-study drug/alcohol screen.
14. History of epilepsy, seizures, other neurological disorders, or neuropsychiatric
conditions.
15. Subjects who have received any prescribed systemic or topical medication within 4
weeks of the first dose administration.
16. Subjects who had used any non-prescribed systemic or topical medication (including
herbal remedies) or megadose vitamins (i.e. 20 to 600 times the recommended daily
supplement dose) within 7 days prior to dosing, unless in the opinion of the study
physician the medication did not interfere with the study procedures or compromise
safety
17. Subjects who have received any medications, including St John's Wort, known to
chronically alter drug absorption or elimination processes within 30 days of the first
dose administration.
18. Regular use of any inducer of metabolism (e.g., barbiturates, rifampin) in the3 months
prior to the first dose administration.
19. Subjects who have participated in a clinical study involving administration of an
investigational drug (new chemical entity) within the following time period prior to
the first dose administration in the current study: 30 days, 5 half-lives or twice the
duration of the biological effect of the investigational product (whichever is
longer).
20. Subjects who consume more than 21 units of alcohol per week or who have a significant
history of alcoholism or drug/chemical abuse (one unit of alcohol equals ½ pint [285
mL] of beer or lager, one glass [125 mL] of wine, or 30 mL of 40% of alcohol by volume
distilled spirits).
21. Excessive consumption of caffeine- or xanthine-containing food or beverages (> 5 cups
of coffee a day or equivalent) or inability to stop consuming from 48 hours prior to
study treatment administration.
22. Subjects who smoked more than 10 cigarettes a day.
23. Any use of drugs-of-abuse or alcohol abuse within 2 years prior to the first admission
to the clinical unit.
24. Inability to understand or communicate reliably with the Investigator or considered by
the Investigator to be unable to or unlikely to co-operate with the requirements of
the study.
25. Any other conditions or factors which in the opinion of the Investigator may interfere
with study conduct. Failure to satisfy the Investigator of fit-ness to participate for
any other reason.
26. Any significant blood loss, donated one unit (450 mL) of blood or more, or received a
transfusion of any blood or blood products within 60 days, or donated plasma within 7
days prior to the first admission to the clinical unit.
27. Inability to refrain from using soft contact lenses starting from administration of
study treatment until follow-up visit.
28. Subjects who are study site employees or immediate family members of the study site or
Sponsor employee.
29. Contraindications related to itraconazole: decompensated heart failure, chronic
obstructive pulmonary disease, concomitant administration of itraconazole and CYP3A4
metabolized drugs known to prolong the QT interval, may result in potentially fatal
ventricular tachyarrhythmias, including torsades de pointe or a life-threatening
arrhythmia. Hypersensitivity or previous adverse events associated with azole
antifungals.
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