| Eligibility |
Inclusion Criteria:
1. Voluntarily sign an ICF and expect to complete the subsequent follow-up.
2. Aged 18 to 65 years (including cut-offs), regardless of gender.
3. B-cell non-Hodgkin's lymphoma diagnosed as CD19-positive by cytology or histopathology
according to WHO 2022 criteria, including pathologically confirmed (1) diffuse large
B-cell lymphoma, non-specific type (DLBCL, NOS); (2) follicular lymphoma
histopathologically graded as grade 3b (FL3b); (3) follicular lymphoma with diffuse
large B-cell transformation; (4) primary mediastinal large B-cell lymphoma (PMBCL);
(5) high-grade B-cell lymphoma (HGBCL).
4. Relapsed/refractory B-cell non-Hodgkin's lymphoma, provided one of the following
conditions is met:
1. Definition of relapse: Relapse after achieving remission (PR and CR) with
second-line or higher therapy;
2. Definition of refractory:
- No response to second-line or more therapy: The best efficacy of last
therapy is PD or SD (SD requires at least 2 cycles of treatment);
- Recurrence (must have biopsy-proven recurrence) or progression within 12
months of autologous stem cell transplantation (ASCT) . If salvage therapy,
no response to last therapy (SD or PD).
5. Subjects must have received adequate treatment in the past, which should include the
following treatments:
1. Anti-CD20 monoclonal antibody, unless the investigator determines that the tumor
is CD20 negative;
2. Chemotherapy containing anthracycline drugs;
3. For subjects with transformed follicular lymphoma (tFL) who have been previously
treated with follicular lymphoma (FL) chemotherapy and with transformation to
DLBCL show refractory to chemotherapy.
6. ECOG performance status 0 to 1.
7. The presence of a measurable lesion that meets one of the following criteria:
1. The long axis of the lymph node lesion exceeds 15 mm in length (the short axis is
measurable);
2. The long and short axes of the extralymph node lesion exceed 10 mm in length.
8. ALaboratory results within 7 days prior to Lymphodepletion need to meet the following
criteria:
1. Coagulation function:
- Activated partial thromboplastin time = 1.5 times the upper limit of normal
(ULN);
- Prothrombin time (PT) = 1.5 times ULN;
2. Liver function:
- Glutathione aminotransferase (AST) = 5 times the upper limit of normal
(ULN);
- Glutamic aminotransferase (ALT) = 5 times the upper limit of normal (ULN);
- Total bilirubin = 1.5 times ULN, unless the subject has documented Gilbert
syndrome;
- Subjects with Gilbert-Meulengracht syndrome with total bilirubin = 3.0 times
ULN and direct bilirubin = 1.5 times ULN may be included.
3. Renal function:
- Serum creatinine = 1.5 times ULN or a creatinine clearance = 60 ml/min;
4. Complete blood count (No blood transfusion treatment received within 7 days prior
to examination):
- Haemoglobin = 80 g/L;
- Absolute neutrophil count (ANC) = 1.0 x 10^9/L;
- A platelet count = 50 x 10^9/L;
5. Cardiopulmonary function:
- Left ventricular ejection fraction (LVEF) = 45%;
- Oxygen saturation = 91%;
9. Female subjects with of childbearing potential should have a negative pregnancy test
during the screening period. Any male and female subjects of childbearing potential
must agree to use an effective contraception method for at least six months from the
time that they sign the informed consent form until the end of the cell infusion.
Female subjects without childbearing potential (meeting at least 1 of the following
criteria) is described below:
1. Have undergone a hysterectomy or bilateral oophorectomy;
2. Medically recognised ovarian failure;
3. Medically recognised as post-menopausal (at least 12 consecutive months of
menopause without pathological or physiological cause).
Exclusion Criteria:
1. Other malignancies within 5 years prior to screening, except adequately treated
carcinoma in situ of the cervix, basal cell or squamous epithelial cell skin cancer,
post-radical localized prostate cancer, post-radical ductal carcinoma in situ, and
post-radical thyroid cancer
2. Any unstable systemic disease: including but not limited to active infection (other
than local infection), unstable angina, cerebrovascular accident or transient
ischaemia (within 6 months prior to screening), myocardial infarction (within 6 months
prior to screening), myocardial infarction (within 6 months prior to screening), New
York Heart Association class III or IV cardiac insufficiency, refractory hypertension
(refractory hypertension is defined as blood pressure that has not reached standard
after >1 month of reasonably tolerable treatment with =3 antihypertensive drugs
(including diuretics) at adequate doses based on lifestyle improvement or blood
pressure that is not effectively controlled with =4 antihypertensive drugs), severe
cardiac arrhythmias requiring pharmacologic treatment, hepatic arrhythmias, liver
diseases, kidney diseases or metabolic disorders.
3. Patients with B-cell non-Hodgkin's lymphoma with active central nervous system
invasion.
4. Patients with positive hepatitis B surface antigen (HBsAg) or hepatitis B core
antibody (HBcAb) and peripheral blood hepatitis B virus (HBV) DNA titres not within
the normal reference range, positive hepatitis C virus (HCV) antibody and peripheral
blood HCV RNA ,positive for human immunodeficiency virus (HIV), or positive for
cytomegalovirus (CMV) DNA, or positive syphilis test.
5. Subjects who are receiving systemic steroids prior to screening and who are judged by
the investigator to require long-term treatment with systemic steroids during the
treatment period (except for inhaled or topical use).
6. Previous organ transplantation or preparation for organ transplantation (except for
haematopoietic stem cell transplantation).
7. Persons with acute/chronic Graft-vs-Host Disease (GvHD).
8. Patients have received a haematopoietic stem cell transplant within 2 months prior to
screening.
9. Active neurological autoimmune or inflammatory diseases (e.g. Guillain-Barre Syndrome
(GBS), Amyotrophic lateral sclerosis (ALS)).
10. Clinically significant active cerebrovascular diseases (such as cerebral edema,
posterior reversible encephalopathy syndrome).
11. Patients with a life expectancy of less than 3 months.
12. Subjects have participated in other interventional clinical studies within 3 months
prior to screening.
13. Received attenuated live vaccine within 6 weeks prior to lymphodepletion.
14. The subjects have contraindications or hypersensitivity reactions to fludarabine,
cyclophosphamide, etoposide, tocilizumab, investigational product and its ingredients.
15. Remaining within the washout period of other antitumor treatments prior to
lymphodepletion.
16. Patients who, in the investigator's judgment and/or clinical criteria, have a
contraindication to any of the study procedures or have other medical conditions that
may place them at unacceptable risk.
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