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NCT04713436 Clinical Trial

The Effects of Antibiotic Combinations on Stem Cells

Anti-Bacterial Agents Clinical Trial

Official title:
The Effects of Antibiotic Combinations on Mesenchymal Stem Cells Derived From Apical Papilla

Clinical Trial Details — Status: Completed

Administrative data
NCT number NCT04713436
Other study ID # 2015-68
Secondary ID
Status Completed
Phase
First received
Last updated
Start date January 1, 2014
Est. completion date February 1, 2017

Study information
Verified date January 2021
Source Eskisehir Osmangazi University
Contact n/a
Is FDA regulated No
Health authority
Study type Observational

Clinical Trial Summary

The purpose of this study was to evaluate the indirect effects of antibiotic combinations. SCAP viability, proliferation and apoptosis of the cells were evaluated. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Description:

The purpose of this study was to evaluate the indirect effects of triple (TAP- ciprofloxacin, metronidazole and minocycline), double (DAP- ciprofloxacin and metronidazole) and modified triple antibiotic pastes (mTAP- ciprofloxacin, metronidazole and cefaclor) on mesenchmal stem cells derived from apical papilla (SCAP). SCAP were isolated from the impacted third molar teeth of the young adults. TAP, DAP and mTAP treatment (14-day medication and 28-day medication) were applied on SCAP and viability, proliferation and apoptosis of the cells were evaluated after four weeks. Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Recruitment information / eligibility
Status Completed
Enrollment 3
Est. completion date February 1, 2017
Est. primary completion date January 1, 2016
Accepts healthy volunteers Accepts Healthy Volunteers
Gender All
Age group 16 Years to 22 Years
Eligibility Inclusion Criteria: - Patients with open apex third molar teeth for extraction indication Exclusion Criteria: - Patients with mature apex third molar teeth for extraction indication

Study Design

Related Conditions & MeSH terms

Intervention

Drug:
• Differentiation Markers
Osteocalcin (BGLAP), dentin sialo phosphoprotein (DSPP) and alkaline phosphatase (ALPP) gene expression levels were also evaluated.

Locations
Country Name City State
Turkey Eskisehir Osmangazi University Eskisehir

Sponsors (2)
Lead Sponsor Collaborator
Eskisehir Osmangazi University TC Erciyes University

Country where clinical trial is conducted

Turkey, 

Outcome
Type Measure Description Time frame Safety issue
Primary Measurment of Cell Viability and Apoptosis To evaluate cell viability and apoptosis MuseCell Analyzer Count and Viability kit (Merck Millipore, Taufkirchen, Germany) was used according to the manufacturer's protocol. 1 year
Primary Measurment of cell differantiation To determine gene expression levels, total RNA of SCAP was extracted using the Ambion PureLink RNA mini kit (Invitrogen, US) and the Roche cell RNA Kit (Penzberg, Germany) according to the manufacturer's instructions. 1 year
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