Comparison of the Genotoxicity of Desflurane and Propofol

Anesthesia Clinical Trial

Official title:

Genotoxic Effects of Desflurane and Propofol Anesthesia in Patients Undergoing Lumbar Disc Surgery

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05185167
• Other study ID #: MSKU 05/III
• Status: Completed
• Phase: N/A
• Start date: January 17, 2022
• Est. completion date: March 20, 2022

Study information

• Verified date: March 2022
• Source: Mugla Sitki Koçman University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The purpose of this study is to evaluate if the anesthetics propofol and desflurane can damage DNA according to comet essay in patients submitted to lumbar disc surgery.

Description:

It is expected that DNA damage information provide the possible genotoxic effects of inhalational and intravenous anesthetics and contribute to the establishment of strategies aimed at reducing the risks to exposed patients.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 30
• Est. completion date: March 20, 2022
• Est. primary completion date: March 15, 2022
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 65 Years

Eligibility

Inclusion Criteria:

1. Ability to understand and willingness to sign an informed consent form

2. Elective lumbar spinal surgery that will take at least 120 minutes,

3. American Society of Anesthesiologists (ASA) physical status I and II

Exclusion Criteria:

1. Patients with ASA physical status III or IV disease

2. Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders)

3. Smoking or alcohol consumption

4. Recently received radiation, chemotherapy

5. Malignancy

6. Patients who will receive blood transfusion during the operation

7. Users of antioxidant supplements

8. Patients who have received general anesthesia in the last three months

9. Patients with known occupational exposures (operating room personnel, chemical plant worker

Related Conditions & MeSH terms

• Anesthesia
• DNA Damage

Intervention

Drug:
• Anesthetics, General Desflurane Maintenance
Comparison of dna damage of desflurane maintenance with propofol maintenance according to comet analysis
• Anesthetics, General Propofol Maintenance
Comparison of dna damage of propofol maintenance with desflurane maintenance according to comet analysis

Locations

Country	Name	City	State
Turkey	Mugla Sitki Kocman University	Mugla

Sponsors (1)

Lead Sponsor	Collaborator
Mugla Sitki Koçman University

Country where clinical trial is conducted

Turkey, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.	Before anesthesia induction
Primary	Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.	2 hours after anesthesia induction
Primary	Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.	postoperative 1st day
Primary	Intensity of the DNA tail detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the intensity of the DNA tail, and the tail greater, the greater the damage.	postoperative 5th day
Primary	Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.	Before anesthesia induction
Primary	Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.	2 hours after anesthesia induction
Primary	Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.	postoperative 1 st day
Primary	Head length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the head length of DNA, the head greater, the smaller the damage.	postoperative 5th day
Primary	Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.	before anesthesia induction
Primary	Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.	2 hours after anesthesia induction
Primary	Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.	postoperative 1 st day
Primary	Tail length of DNA detected by using Comet Assay IV Version 4.3.2 for Basler FireWire	The blood samples that evaluate with comet analysis aim to detect the tail length of DNA, the head greater, the greater the damage.	postoperative 5th day