Colorectal Neoplasms Clinical Trial
Official title:
Diagnostic Potential of Hypermethylated DNA in Colorectal Cancer
Colorectal cancer (CRC) is one of the most common forms of cancer and the second leading
cause of cancer-related deaths in the western world.
CRC mortality is related to stage of disease with a five year survival for early-stage
disease of 77.0% and 50.8% for late stage disease. Methods for early detection of primary as
well as recurrent CRC are therefore important to increase patient survival. Tumour
biomarkers from blood, stool, or urine could aid the early diagnostics of CRC, but despite
extensive research such markers have only provided limited clinical value.
Sporadic CRC develops as a result of the accumulation of genetic and epigenetic alterations.
Epigenetic alterations include DNA hypermethylation, which through transcriptional silencing
of tumour suppressor genes is associated with cancer development and cancer progression. The
search for gene promoter regions hypermethylated in cancer has been ongoing for nearly two
decades, and a number of genes have been shown to be preferentially hypermethylated in CRC.
Therefore, hypermethylated DNA in plasma has been suggested as a marker for tumour-stage and
survival in CRC patients. The only approved biomarker for the detection of CRC recurrence is
the protein carcinoembryonic antigen (CEA). CEA is limited by its low sensitivity and
therefore not recommended as a diagnostic biomarker. Hypermethylation of CRC specific genes
as part of a molecular biomarker panel measured in blood could prove to be a recurrence
marker in CRC patients, with elevated sensitivity and specificity.
The aims of this project are to examine if hypermethylation of specific genes measured from
cell-free DNA in plasma of CRC patients can be used to detect primary CRC, to detect CRC
recurrence and to be a biomarker for CRC prognosis.
Development of a reliable sensitive and specific biomarker for CRC will immensely improve
the diagnostics and handling of CRC patients.
Colorectal cancer (CRC) is one of the most common forms of cancer and the second leading
cause of cancer-related deaths in the western world, accounting for the annual death of
215.000 europeans in 2012 and an estimated 50.310 Americans in 2014.
Sporadic CRC develops through the accumulation of genetic and epigenetic alterations in the
epithelial cell-lining. These alterations are thought to push the normal epithelial cells
into the adenoma-carcinoma sequence. Detection of hypermethylated DNA can be made in blood
or stool of cancer patients and it is believed, that hypermethylated cell-free DNA in blood
and stool can be used as a cancer biomarker, exhibiting high sensitivity and specificity in
CRC and other major cancer-types (e.g. lung, prostate, and breast cancer).
Hypermethylated cell-free DNA - measured in plasma - could prove to be a more specific and
sensitive biomarker for CRC than both the detection of blood in stool and CEA in blood of
CRC patients.
The aims of this project are to examine if hypermethylation of specific genes measured from
cell-free DNA in plasma of CRC patients is a diagnostic biomarker for CRC. The results of
this study will show, if a single blood sample can differentiate patients with CRC from
patients without cancer.
Development of a reliable sensitive and specific biomarker for CRC will immensely improve
the diagnostics and handling of CRC patients.
To propose the most sensitive and specific hypermethylated DNA promoter regions for CRC
detection, a review of the literature currently available will be undertaken. The following
search criteria (MeSH terms) will be used: "colorectal neoplasm", "DNA methylation or CpG
islands", "tumour or stool sample or blood or serum or plasma", "real time PCR or microarray
analysis or genome sequencing or biopsy or blood analysis". Based on this review a
literature review will be written: "DNA Methylation in Blood and Stool as a Screening and
Prognostic Marker for Colorectal Cancer".
Epigenetics is the study of heritable changes in gene expression, which does not arise due
to changes in the DNA sequence. Epigenetic alterations include micro-satellite instability,
histone modifications and DNA methylation. DNA methylation is a normal phenomenon in
eukaryotic cells and occurs across the whole genome. Methylation target cytosine-residues
followed by a guanine on the DNA strand (called CpG-islands) converting these to
methyl-cytosine. Un-methylated CpG-islands exist in a high concentration in the promoter
regions of human genes, and DNA promoter hypermethylation leads to decreased transcriptional
activity. This event happens in tumour suppressors leading to gene silencing which is
related to tumour genesis. CpG-Island hypermethylation is shown to be a non-random process
and the pattern of gene hypermethylation appears to be specific to individual cancers. This
lead to the definition of a CpG-Island Methylator Phenotype (CIMP) in CRC, a panel of genes
being hypermethylated in CRC tumours, and there is a growing body of literature on the
correlation between hypermethylated genes and CRC. This investigation has primarily focused
on DNA obtained from CRC biopsies but in recent years focus has moved to hypermethylated DNA
obtained from blood and stool samples.
Hypermethylated genes that have been analysed in blood of CRC patients are e.g. SDC2, THBD,
HIC1, RASSF1A. Hypermethylated SEPT9 has been analysed in a large prospective study to
evaluate its performance as a screening marker, but this only revealed a sensitivity of
48.2% with a specificity of 91.5%. However when hypermethylated promoter regions are
analysed in concert with others as part of a biomarker panel, markedly better performance
has been reported. A biomarker panel composed of RASSF1A, APC, MGMT, and Wif-1 reached a
sensitivity of 86.5% and a specificity of 92.1% for CRC detection.
From these studies, it is apparent that the profile of hypermethylated DNA in blood samples
from CRC patients is different from the profile measured in healthy controls.
Only two studies have been conducted, prospectively studying the relationship between DNA
hypermethylation measured in blood of CRC patients and risk of cancer recurrence. These
studies are limited by only analysing a single gene promoter region (p16) and a low number
of study participants.
The studies above show that hypermethylated DNA can be detected in blood of CRC patients,
promoting it as biomarker for CRC. No study has however prospectively analysed the
performance according to treatment and relapse of disease in CRC patients.
Studies on hypermethylated DNA in stool of CRC patients have been conducted using single
gene hypermethylation markers. The gene primarily analysed has been hypermethylated VIM
which also has a commercially available kit for stool analysis. The focus in the stool
studies has been DNA hypermethylation as a screening marker for CRC. One study combined the
iFOBT test with hypermethylation of the PHACTR3 gene, extracted from the iFOBT fluid. A
positive result in at least one of the two tests raised sensitivity to 95% (19/20) at high
specificity of 94% (45/48) for CRC and advanced adenomas.
Most of the methods for analysis require bisulfite conversion of the DNA for detection of
methylated cytosines, and Pedersen et al. (2012) recently published an article addressing
this key time-consuming step lowering the analysis-time and increasing the recovery of
sparse amounts of DNA (< 0.1 ng/ml), rendering the test more clinically applicable.
In summary, DNA collected from blood and stool samples of CRC patients has been analysed
with the purpose of discovering hypermethylated genes that are CRC specific.
Hypermethylation of these specific genes in blood samples have been associated with poor
prognosis (p16, HLTF, TMEFF1, ALX4, VIM, and FBN2), early diagnosis (APC, TAC1, SEPT9,
NEUROG1, RASSF1A, SDC2, and THBD) and potential recurrence markers for CRC patients (p16 and
TFPI2). Especially THBD and SDC2 show great promise as markers of early stage CRC, and the
fact that it seems possible to use DNA methylation in blood as a marker of disease
recurrence warrants further research, using these genes individually or in concert with
others. Hypermethylation of DNA in stool samples of CRC patients have not been analysed as
thoroughly as in blood samples, though detection of hypermethylated DNA fragments in stool
samples could be used when screening for CRC. SFRP2, TMEFF2, SPG20, TFPI2, and PHACTR3 have
shown promise in the detection of CRC at early stages or in premalignant lesions. The fact
that PHACTR3 could be detected in iFOBT-fluid (reaching increased sensitivity and
specificity) indicates a possible new screening method, where DNA hypermethylation is
analysed in one stool sample. There are however issues regarding the analytical sensitivity
of hypermethylated DNA in stool-samples from CRC patients. The diagnostic sensitivity for
right-sided tumours is lower than for left-sided tumours suggesting DNA degradation in the
colonic lumen before stool excretion. This promotes blood as the media of choice for
detection of hypermethylated genes as biomarkers in the diagnosis, prognosis and recurrence
detection of CRC.
METHOD AND STUDY PARTICIPANTS
1. To propose the most sensitive and specific hypermethylated DNA promoter regions for CRC
detection, a review of the literature currently available will be undertaken. The
search criteria are listed in the previous section.
2. Colorectal cancer patients:
Blood samples from approximately 210 patients diagnosed with colorectal carcinoma
collected prospectively and consecutively at the Department of Surgical
Gastroenterology, Aalborg University Hospital during the time period 2003-2006 will be
used. The blood samples were collected at the time of diagnosis, before and after
treatment (surgery and radio-chemo-therapy) and at regular intervals for a time period
of two years after treatment. These blood samples were collected during a previous
PhD-study: "Pre- and postoperative Venous Thromboembolism in Patients with Colorectal
Cancer - implications of Radiotherapy" (ID-number VN 2003/102). In collaboration with
the Section of Molecular Diagnostics, Clinical Biochemistry, Aalborg University
Hospital, the methylation profile of the cell-free DNA promoter regions in plasma will
be analysed using methylation specific PCR (Polymerase Chain Reaction) based on an
accelerated bisulfite treatment method.
3. Inflammatory bowel disease patients In collaboration with The Department of Medical
Gastroenterology, Aalborg University Hospital, there will be recruited approximately
100 patients with ulcerous colitis for blood sampling (28 ml). In collaboration with
the Section of Molecular Diagnostics, Clinical Biochemistry, Aalborg University
Hospital, the methylation profile of the cell-free DNA promoter regions in plasma will
be analysed using methylation specific PCR (Polymerase Chain Reaction) based on an
accelerated bisulfite treatment method.
4. Control group:
Blood samples (8ml) from patients (n=142) who underwent work-up for lower
gastrointestinal malignancy during the time period 2003-2006. Colonoscopy was
performed, and no malignancy or premalignant lesions found. Blood samples were
collected before/after colonoscopy. These blood samples were collected during a
previous PhD-study: "Pre- and postoperative Venous Thromboembolism in Patients with
Colorectal Cancer - implications of Radiotherapy" (ID-number VN 2003/102). The plasma
samples will be analysed at the Section of Molecular Diagnostics, Clinical
Biochemistry, Aalborg University Hospital, and the methylation profile of the cell-free
DNA in plasma will be analysed, using methylation specific PCR based on the accelerated
bisulfite treatment protocol.
5. External validation:
Cases:
Blood samples from 143 patients were collected prospectively and consecutively for a
previous study on the effect of omega 3 fatty acids an postoperative complications
after colorectal surgery. All study participants have provided written informed consent
(N-VN-20050035).
Controls:
There will be recruited approximately 100 control subjects with a positive occult
faecal blood but with a normal colonoscopy for blood sampling (28 ml). In collaboration
with the Section of Molecular Diagnostics, Clinical Biochemistry, Aalborg University
Hospital, the methylation profile of the cell-free DNA promoter regions in plasma will
be analysed using methylation specific PCR (Polymerase Chain Reaction) based on an
accelerated bisulfite treatment method.
Inclusion criteria:
Patients referred to The Department of Gastrointestinal Surgery, Aalborg University
Hospital, with a positive stool test for CRC but with no sign of cancer on the
subsequent colonoscopy.
6. Blood sampling:
Blood samples will be drawn by venepuncture in accordance with the European concerted
action on thrombosis (ECAT) procedures.
7. Methylation specific PCR:
In collaboration with the Section of Molecular Diagnostics, Clinical Biochemistry,
Aalborg University Hospital the cell-free DNA promoter regions methylation profile will
be analysed in EDTA plasma samples by methylation specific PCR based on the accelerated
bisulfite protocol.
8. Biobank Any residual material (blood samples) from the study will be stored for fifteen
years and thereafter the remaining samples will be transferred to the Danish National
Biobank.
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