25(OH)D Measurement and 25(OH)D Deficiency Clinical Trial
Official title:
LC-MS/MS Based 25(OH)D Status in a Large Central European Outpatient Cohort- Gender and Age Specific Differences
The present study assessed serum 25(OH)D in a cohort of 74235 outpatients by
liquid-chromatography-tandem-mass-spectrometry (LC-MS/MS).
The investigators studied the distribution of serum 25(OH)D concentrations in males and
females of different age groups, the prevalence of measurable amounts of 25(OH)D2 and
seasonal variability.
Measurements were performed under routine circumstances. Samples were collected in serum tubes with clot-activator. As per routine procedure, samples were centrifuged upon arrival in the lab and stored at 4° C until measurement. 25(OH)D3 and 25(OH)D2 were quantitated separately. Results from subjects <18 years were excluded from the analysis. The study was approved by the local Ethics Committee. ;