Sepsis Clinical Trial
Official title:
The Effect of Glutamine Infusion on the Inflammatory Response and HSP-70 in BMNCs During Human Experimental
Glutamine levels decrease during severe sepsis; this may be associated with increased
mortality. The investigators tested the effects of glutamine supplementation on systemic
inflammation in a human sepsis model.
The investigators found that glutamine levels drops significantly during experimentally
induced systemic inflammation. However, glutamine did not affect the degree of inflammation.
Glutamine levels have been shown to decrease substantially with severe sepsis and this has
been connected with increased mortality. Therefore, in the present study, we infused either
saline or Alanine-glutamine during an endotoxin challenge and measured parameters related to
an immune response, i.e. plasma cytokines and Heat Shock Protein (HSP)-70.
Materials and Methods This was a double blind, randomised, placebo-controlled crossover
trial in eight healthy young men. The study was performed in random order on two separate
days, with a four-week washout period between days. Subjects received an infusion of
Alanine-glutamine ( Dipeptiven) at a rate of 0.025 g / (kg BW * h) for 10 hrs or saline.
After two hours of infusion subjects received an intravenous bolus of E. coli endotoxin (0.3
ng/kg). Blood samples were collected hourly for the following eight hours. HSP-70 protein
content in isolated Blood Mononuclear Cells (BMNCs) was measured by western blotting.
Results and Discussion Plasma glutamine was significantly increased during infusion with
alanine-glutamine infusion. En-dotoxin caused a reduction in plasma-glutamine during saline
infusion as well as during Alanine-glutamine infusion. A significant effect of endotoxin was
found on leukocyte subpopulations, tumor necrosis factor-a, interleukin-6, the expression of
HSP-70 in BMNCs, temperature, and heart rate. However, no differences were detected between
treatments with regard to the effect of endotoxin on any of these parameters.
Conclusion Endotoxemia reduces plasma glutamine independently of parenteral infusion of
alanine-glutamine. Glutamine does not alter the response of leukocytes, leukocyte
subpopulations, IL-6, or TNF-α, or the expression of HSP-70 in BMNCs to endotoxemia.
;
Allocation: Randomized, Intervention Model: Crossover Assignment, Masking: Double Blind (Subject, Investigator), Primary Purpose: Basic Science
| Status | Clinical Trial | Phase | |
|---|---|---|---|
| Active, not recruiting |
NCT05095324 -
The Biomarker Prediction Model of Septic Risk in Infected Patients
|
||
| Completed |
NCT02714595 -
Study of Cefiderocol (S-649266) or Best Available Therapy for the Treatment of Severe Infections Caused by Carbapenem-resistant Gram-negative Pathogens
|
Phase 3 | |
| Completed |
NCT03644030 -
Phase Angle, Lean Body Mass Index and Tissue Edema and Immediate Outcome of Cardiac Surgery Patients
|
||
| Completed |
NCT02867267 -
The Efficacy and Safety of Ta1 for Sepsis
|
Phase 3 | |
| Completed |
NCT04804306 -
Sepsis Post Market Clinical Utility Simple Endpoint Study - HUMC
|
||
| Recruiting |
NCT05578196 -
Fecal Microbial Transplantation in Critically Ill Patients With Severe Infections.
|
N/A | |
| Terminated |
NCT04117568 -
The Role of Emergency Neutrophils and Glycans in Postoperative and Septic Patients
|
||
| Completed |
NCT03550794 -
Thiamine as a Renal Protective Agent in Septic Shock
|
Phase 2 | |
| Completed |
NCT04332861 -
Evaluation of Infection in Obstructing Urolithiasis
|
||
| Completed |
NCT04227652 -
Control of Fever in Septic Patients
|
N/A | |
| Enrolling by invitation |
NCT05052203 -
Researching the Effects of Sepsis on Quality Of Life, Vitality, Epigenome and Gene Expression During RecoverY From Sepsis
|
||
| Terminated |
NCT03335124 -
The Effect of Vitamin C, Thiamine and Hydrocortisone on Clinical Course and Outcome in Patients With Severe Sepsis and Septic Shock
|
Phase 4 | |
| Recruiting |
NCT04005001 -
Machine Learning Sepsis Alert Notification Using Clinical Data
|
Phase 2 | |
| Completed |
NCT03258684 -
Hydrocortisone, Vitamin C, and Thiamine for the Treatment of Sepsis and Septic Shock
|
N/A | |
| Recruiting |
NCT05217836 -
Iron Metabolism Disorders in Patients With Sepsis or Septic Shock.
|
||
| Completed |
NCT05018546 -
Safety and Efficacy of Different Irrigation System in Retrograde Intrarenal Surgery
|
N/A | |
| Completed |
NCT03295825 -
Heparin Binding Protein in Early Sepsis Diagnosis
|
N/A | |
| Not yet recruiting |
NCT06045130 -
PUFAs in Preterm Infants
|
||
| Not yet recruiting |
NCT05361135 -
18-fluorodeoxyglucose Positron Emission Tomography/Computed Tomography in S. Aureus Bacteraemia
|
N/A | |
| Not yet recruiting |
NCT05443854 -
Impact of Aminoglycosides-based Antibiotics Combination and Protective Isolation on Outcomes in Critically-ill Neutropenic Patients With Sepsis: (Combination-Lock01)
|
Phase 3 |