Analysis of Gingival Crevicular Fluid

Periodontal Diseases Clinical Trial

— GCF  

Official title:

Analysis of Gingival Crevicular Fluid IL-1β, IL-10, IL-36γ Cytokine Levels in Gingivitis and Periodontitis Patients

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05325905
• Other study ID #: 2014/01
• Status: Completed
• Start date: October 2014
• Est. completion date: January 2017

Study information

• Verified date: April 2022
• Source: Bezmialem Vakif University
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.

Description:

Gingival crevicular fluid (GCF) is formed when fluid exudes from the vessels of the microcirculation into the inflamed periodontal tissue and into the sulcus or pocket. As the fluid traverses the inflamed tissue, it is thought to pick up enzymes and other molecules that participate in the destructive process, as well as products of cell and tissue degradation. Efforts to develop diagnostic tests based on host factors have been focused almost entirely on analysis of GCF.

The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.

Systemically healthy and non-smokers 80 participants (30 males and 50 females) who were selected from among admitting to Dentistry Faculty of Selcuk University were included in this study. Clinical periodontal parameters were recorded and GCF samples were obtained. Samples were stored in -80 ˚C until analysis with Enzyme-Linked ImmunoSorbent Assay (ELISA).

Recruitment information / eligibility

• Status: Completed
• Enrollment: 81
• Est. completion date: January 2017
• Est. primary completion date: April 2016
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 20 Years and older

Eligibility

Inclusion Criteria:

• Systemically healthy

• non-smokers,

• presence of at least 20 teeth in the mouth

Exclusion Criteria:

• receiving periodontal therapy in the last 6 months;

• receiving antibiotics or anti-inflammatory drugs in the last 6 months;

• currently pregnant or breastfeeding

• using a removable partial denture and removable or fixed orthodontic appliances.

Related Conditions & MeSH terms

• Periodontal Diseases

Intervention

Diagnostic Test:
• Gingival crevicular fluid sampling
GCF sampling and clinical periodontal measurements were performed

Locations

Country	Name	City	State
n/a

Sponsors (2)

Lead Sponsor	Collaborator
Bezmialem Vakif University	Selcuk University

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Interleukin-36? (IL-36?) levels in GCF	GCF samples were obtained from one interproximal site in each quadrant	Baseline
Secondary	Probing depth (PD)	PD; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars.	Baseline
Secondary	Clinical attachment level (CAL)	CAL; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars.	Baseline
Secondary	Bleeding on Probing (BoP)	BoP, from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars	Baseline
Secondary	IL-10 and IL-1ß levels in GCF	GCF samples were obtained from one interproximal site in each quadrant	Baseline
Secondary	Plaque Index (PI)	PI; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars	Baseline
Secondary	Gingival Index (GI)	GI; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars	Baseline