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Clinical Trial Details — Status: Completed

Administrative data

NCT number NCT01769625
Other study ID # 200807-001
Secondary ID
Status Completed
Phase Phase 1/Phase 2
First received January 14, 2013
Last updated August 8, 2017
Start date January 2009
Est. completion date November 2016

Study information

Verified date August 2017
Source Hartford Hospital
Contact n/a
Is FDA regulated No
Health authority
Study type Interventional

Clinical Trial Summary

This is a biomarker study with the goal of measuring changes in proteins and gene methylation. This study is not intended for use in diagnosing, mitigating, treating, curing, or preventing disease.

The purpose of this study is to determine if Vitamin D (cholecalciferol) alone and in combination with celecoxib (Celebrex, a non-steroidal anti-inflammatory drug, or NSAID), act together to decrease breast cancer risk by their effect on certain biological indicators (biomarkers) of breast cancer risk (called PGE2, COX-2, and 15-PGDH) and cell changes in the breast.


Description:

This is a biomarker study with the goal of measuring changes in protein and rna expression. This study is not intended for use in diagnosing, mitigating, treating, curing, or preventing disease.

66 women at normal risk for developing breast cancer will be recruited and enrolled. 22 women will be randomized into each arm, with anticipation of 2 women in each group will not be evaluable, leaving 20 in each group for evaluation.

A combination of vitamin D and celecoxib act synergistically to decrease breast cancer risk by decreasing cell proliferation in the mammary epithelium through their action on prostaglandin synthesis and metabolism.

Specific Aims:

-Evaluate vitamin D metabolism, through the measurement of CYP24 in the breast.

2-Evaluate breast specific levels of vitamin D and celecoxib, and assess if the levels of these compounds correlate with response to markers which influence prostaglandin synthesis and metabolism. Additionally, in women without active breast cancer , we will determine the effect of vitamin D, with or without celecoxib, on 1) PG synthesis and metabolism, through the measurement of PGE2, COX-2 and 15-PGDH in the breast, 2) proliferative activity in the breast,, and 3) circulating levels of vitamin D and celecoxib, to determine if levels of these compounds correlate with response to markers of PG production, metabolism, or cell proliferation.


Recruitment information / eligibility

Status Completed
Enrollment 31
Est. completion date November 2016
Est. primary completion date November 2016
Accepts healthy volunteers Accepts Healthy Volunteers
Gender Female
Age group 18 Years and older
Eligibility Inclusion Criteria:

- Woman >18 years old

- Healthy women who are at normal risk for developing breast cancer

- ECOG Performance Status score 0-1

- Premenopausal women must not be pregnant

Exclusion Criteria:

- History of bilateral mastectomy, or bilateral breast irradiation

- Significant medical or psychiatric problems making the participant a poor candiate

- Evidence of excess use of narcotics or drug dependency

- Have been pregnant and lactating in the past 2 years

- Significant history of peptic ulcer disease or upper gastrointestinal bleeding

- History of severe congestive heart failure that requires hospitalization or intervention

- History of asthma requiring medication for treatment

- Allergy to sulfonamides or NSAID medications

- History of myocardial infarction or stroke

- Currently on Coumadin

- Currently on Tamoxifen (nolvadex),Evista (raloxifene), Femara (letrozole), Arimidex (anastrozole), or Aromasin (exemestane)

- Undergone prior subaeolar breast surgery

Study Design


Related Conditions & MeSH terms


Intervention

Drug:
placebo/celecoxib 400 mg and cholecalciferol 400 IU/cholecalciferol 2,000 IU
Take 1 capsule from each bottle (1 bottle containing either placebo/celecoxib and 1 bottle containing either cholecaliferol 400 IU/cholecaliferol 2,000 IU) for 30 days

Locations

Country Name City State
United States University of North Dakota Grand Forks North Dakota

Sponsors (2)

Lead Sponsor Collaborator
Hartford Hospital Susan G. Komen Breast Cancer Foundation

Country where clinical trial is conducted

United States, 

Outcome

Type Measure Description Time frame Safety issue
Primary PG synthesis and metabolism, through the measurement of PGE2, COX-2 and 15-PGDH in the breast This will be measured from both baseline and completion samples
1. PG synthesis and metabolism, through the measurement of 15-PGDH, COX-2, and PGE2 in the breast
Rationale: 1,25(OH)2D, the active form of vitamin D, has been shown in vitro to decrease PGE2 both by interfering with its production and by increasing its breakdown, leading to lower cell proliferation. Celecoxib potentiated the antiproliferative effect, allowing a much lower dose of each agent when used in combination than in isolation.
approximately 30 days
Secondary proliferative activity in the breast and circulating levels of vitamin D and celecoxib, to determine if levels of these compounds correlate with response to markers of PG production, metabolism, or cell proliferation. This will be measured from both baseline and completion samples.
2. Proliferative activity in the breast, as measured by MD cell morphology
Rationale: Both MD and NAF contain ductal epithelial cells, but MD samples contain more cells for cytologic review than NAF. Findings on MD cytology correlate with likelihood of breast cancer, NAF cytology relates to breast cancer risk and improves risk stratification, and bioactive food components can alter NAF cytology
approximately 30 days