Analysis of Proteome and Pathophysiological Characteristics of Human Blood Cells

Peripheral Blood Mononuclear Cell Clinical Trial

Official title:

Analysis of Proteome and Pathophysiological Characteristics of Human Blood Cells

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT02272530
• Other study ID #: Proteome
• Status: Completed
• Start date: July 2012
• Est. completion date: December 31, 2020

Study information

• Verified date: February 2021
• Source: Heinrich-Heine University, Duesseldorf
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The aim of this study is to investigate wether change in endothelial nitric oxid synthase of blood cells (erythrocytes and peripheral blood mononuclear cells (PBMC)) has an influence on development and progression of cardiovascular diseases.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 200
• Est. completion date: December 31, 2020
• Est. primary completion date: December 31, 2020
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 20 Years to 75 Years

Eligibility

Inclusion Criteria:

• healthy volunteers
• Patients: stable coronary artery disease with accordingly endothelial dysfunction

Exclusion Criteria:

• acute inflammation (CRP>0,5 mg/dl, leucocyte >11000/dl)
• malignant diseases
• acute or terminated kidney disease
• relevant heart failure (NYHA III-IV)

Related Conditions & MeSH terms

• Endothelial NO-synthase
• Peripheral Blood Mononuclear Cell
• Red Blood Cell

Locations

Country	Name	City	State
Germany	University Hospital Duesseldorf	Duesseldorf

Sponsors (1)

Lead Sponsor	Collaborator
Heinrich-Heine University, Duesseldorf

Country where clinical trial is conducted

Germany, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Blood examination	Following parameters will be examined: Blood count (red cells, white cells, platelets, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width (RDW), mean platelet volume (MPV) ); Creatinin; Urea; Glucose; Glycated hemoglobin (HbA1c); Cholesterol; Triglyceride; C-reactive Protein; Apolipoprotein	baseline
Secondary	Protein expression	Determination with western blot	baseline
Secondary	Asymmetric dimethylarginine (ADMA) and Cytokine	Determination with enzyme-linked immunosorbent assay (ELISA).	baseline
Secondary	Microparticles	Determination with ELISA and flow cytometry	baseline
Secondary	Immunocytochemistry		baseline
Secondary	Nitric oxid metabolite and proteins in blood plasma, red blood cells and peripheral blood mononuclear cells (PBMN)	Determination with reductive chemiluminescence	baseline
Secondary	Nitric oxid and reactive oxygen species	Determination with fluorescence coloring in flow cytometry and fluorescence microscopy	baseline