Human Brown Adipose Tissue and Mitochondrial Respiration

Obesity Clinical Trial

Official title:

Human Brown Adipose Tissue Histological and Mitochondrial Respiratory Analysis

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT03111719
• Other study ID #: NL31367.068.10
• Status: Recruiting
• Start date: January 4, 2010
• Est. completion date: October 10, 2021

Study information

• Verified date: February 2020
• Source: Maastricht University Medical Center
• Contact: Wouter D Van Marken Lichtenbelt, Professor
• Phone: +31(0)433881629
• Email: markenlichtenbelt[@]maastrichtuniversity.nl
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

This study is focused on measuring mitochondrial activity of human brown adipose tissue. During surgery, adipose tissue biopsies from white and brown adipose tissue will be collected from the deep neck area. These biopsies will be used to examine mitochondrial function via histological analysis, biochemical analysis and in vitro experiments based on precursor cells present in the biopsies.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 180
• Est. completion date: October 10, 2021
• Est. primary completion date: October 10, 2021
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years to 65 Years

Eligibility

Inclusion Criteria:

• Indication for surgery in the supraclavicular and neck region (more specific patients diagnosed with hyperparathyroidism, struma, benign thyroid gland tumours, implantation of a vagal neurostimulator for epilepsia and cervical neurologic disorders)

• In the thyroid gland group, euthyroid function

Exclusion Criteria:

• Pre-operatively diagnosed malign tumour

• Patients undergoing acute surgery because of critical illness

Related Conditions & MeSH terms

• Adipose Tissue, Brown
• Aging
• Metabolic Diseases
• Mitochondrial Metabolism Disorders
• Obesity

Locations

Country	Name	City	State
Netherlands	Maastricht University Medical Center / University of Maastricht	Maastricht

Sponsors (2)

Lead Sponsor	Collaborator
Maastricht University Medical Center	Ministry of Education, Culture and Science

Country where clinical trial is conducted

Netherlands, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Mitochondrial respiration in human brown	Measure of mitochondrial respiration in human brown adipose tissue biopsies and measure mitochondrial respiration in cells derived from human brown adipose tissue biopsies. With high resolution respirometry , oxygen consumption in mitochondria will be determined using different substrates that stimulate mitochondrial activity including norepinephrine which is a natural agonist to increase mitochondrial activity in brown adipose tissue. During these experiments mitochondrial complex activity can be inhibited using specific inhibitors.	8 weeks
Secondary	Characterise human brown adipose tissue	Perform histological analysis and biochemical analysis in brown adipose tissue biopsies and compare to skeletal muscle. Via qPCR analysis, western blotting and immunfluorescence we will examine UCP1 expression in BAT.	8 weeks