Natural Killer(NK) Cell Therapy in Acute Myeloid Leukemia

Minimal Residual Disease Clinical Trial

Official title:

Phase I Study to Evaluate the Safety and Efficacy of NK Cell Therapy in Acute Myeloid Leukemia (AML).

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT05987696
• Other study ID #: QH-TJ-01
• Status: Not yet recruiting
• Phase: Phase 1
• Start date: August 10, 2023
• Est. completion date: August 31, 2026

Study information

• Verified date: August 2023
• Source: Institute of Hematology & Blood Diseases Hospital
• Contact: Jianxiang Wang
• Phone: 022-23909120
• Email: wangjx[@]ihcams.ac.cn
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This is a phase 1, first-in-human (FIH), open-label, multicohort study to evaluate the safety, tolerability and preliminary efficacy of iPSC NK cells in patients with relapsed/refractory AML or AML Minimal Residual Disease (MRD).

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 102
• Est. completion date: August 31, 2026
• Est. primary completion date: August 31, 2026
• Accepts healthy volunteers: No
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

1. Provision of signed and dated informed consent form (ICF).

2. =18 years old.

3. Eastern Cooperative Oncology Group (ECOG) performance status =1 and life expectancy greater than 12 weeks.

4. Diagnosis of r/r AML (Cohort 1 and 2) or AML MRD (Cohort 3).

5. Cohort 1: Both CLL1 and CD33 expression are positive in AML blasts; Cohort 2: The expression of CD33 in AML blast is positive.

6. Adequate organ and marrow function, as defined below:

1. Blood creatinine (Cr) = 2 x ULN or calculated creatinine clearance (Cockcroft-Gault formula) = 50 mL/min;

2. Total bilirubin (TBIL) = 2 x the ULN;

3. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) = 3 x ULN;

4. International normalized ratio (INR) and activated partial thromboplastin time (aPTT) = 1.5 x ULN;

7. Females of childbearing potential must have a negative serum pregnancy test.

8. Donor specific antibody (DSA) is negative: MFI <= 2000.

Exclusion Criteria:

1. Allergic to drug used in this study.

2. Subjects received any antitumor therapy as follows, prior to first NK infusion:

1. Systemic steroid therapy within 3 days (except physiological replacement therapy);

2. Systemic antitumor therapy within 2 weeks or at least 5 half-lives, whichever is less;

3. Radiotherapy within 4 weeks;

4. Donor lymphocyte infusion within 6 weeks;

5. Intrathecal treatment within 1 week;

6. CAR-T therapy, CAR-NK therapy, or any other genetically modified cell therapy product within 6 months;

3. History of allogeneic stem cell transplantation.

4. Received the vaccine within 4 weeks prior to the first infusion and/or expected to require vaccination from the study period to 12 weeks after the last infusion.

5. Active central nervous system Leukemia.

6. Acute Promyelocytic Leukemia (APL).

7. History of other malignant tumors, except for those who have achieved complete remission more than 5 years after radical treatment without any signs of recurrence.

8. Active autoimmune diseases.

9. History of central nervous system disease or meningeal involvement such as epilepsy, paralysis, aphasia, stroke, etc.

10. Serious cardiovascular and cerebrovascular diseases:

1. Severe heart rhythm or conduction abnormalities, corrected QT interval (QTc)=480 ms;

2. Acute coronary syndrome, congestive heart failure, aortic dissection, stroke, or other grade 3 or higher cardiovascular and cerebrovascular events within 6 months prior to first infusion;

3. New York Heart Association (NYHA) class II or above congestive heart failure or left ventricular ejection fraction (LVEF) <50% in color Doppler echocardiography;

4. Hypertension that cannot be controlled by drug.

11. Active pulmonary infection; SpO2 =90%; Pulmonary embolism, chronic obstructive pulmonary disease, or interstitial lung disease.

12. Uncontrolled bacterial, fungal, or viral infection. Known HIV infection, active Hepatitis B (HBV) or Hepatitis C (HCV) infection.

13. History of substance abuse.

14. Toxicity induced by previous therapy not recovered to = grade 2(NCI-CTCAE v5.0).

15. Large surgical treatment within 4 weeks prior to first infusion, not including diagnostic biopsy.

16. Pregnant/breastfeeding women.

17. Investigator-assessed presence of any medical or social issues that are likely to interfere with study conduct or may cause increased risk to subject.

Related Conditions & MeSH terms

• AML, Adult
• Leukemia, Myeloid, Acute
• Minimal Residual Disease
• Neoplasm, Residual

Intervention

Drug:
• CD33/CLL1 dual CAR-NK cell
NK cell therapy
• Cyclophosphamid
Lympho-conditioning Agent
• Fludarabine
Lympho-conditioning Agent
• Cytarabine
Lympho-conditioning Agent
• CD33 CAR-NK cell
NK cell therapy
• super NK cell
NK cell therapy

Locations

Country	Name	City	State
China	Institute of Hematology & Blood Diseases Hospital	Tianjin

Sponsors (2)

Lead Sponsor	Collaborator
Institute of Hematology & Blood Diseases Hospital	Hangzhou Qihan Biotech Co.,Ltd.

Country where clinical trial is conducted

China, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Incidence of Treatment-Emergent Adverse Events [Safety and Tolerability]		28 Days from first dose of iPSC NK cell infusion
Primary	Incidence of subjects with Dose Limiting Toxicities within each dose level cohort		28 Days from first dose of iPSC NK cell infusion
Secondary	Overall Response Rate(ORR)		Up to approximately 2 years after last dose of iPSC NK cell infusion
Secondary	MRD negative rate		28 Days from first dose of iPSC NK cell infusion
Secondary	Event-free survival		Up to approximately 2 years after last dose of iPSC NK cell infusion]
Secondary	Relapse-free survival		Up to approximately 2 years after last dose of iPSC NK cell infusion
Secondary	Overall survival (OS)		Up to approximately 2 years after last dose of iPSC NK cell infusion
Secondary	Determination of the pharmacokinetics (PK) of iPSC NK cells in peripheral blood	The PK of iPSC NK in peripheral blood will be reported as the relative percentage of product DNA versus patient DNA (% chimerism) measured from blood samples at the specified time points.	Up to approximately 2 years after last dose of iPSC NK cell infusion