Evaluation of Oxidative Stress and Inflammation Markers in Temporomandibular Disorders

Inflammation Clinical Trial

Official title:

The Evaluation of Oxidative Stress and Inflammation Markers in Serum and Saliva of the Patients With Temporomandibular Disorders

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05985733
• Other study ID #: PYO.DIS.1904.17.018
• Status: Completed
• Start date: June 19, 2020
• Est. completion date: April 19, 2023

Study information

• Verified date: August 2023
• Source: Bahçesehir University
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

Background: The occurrence of many diseases has been associated with oxidative stress and disruption of antioxidant mechanisms. Temporomandibular Joint(TMJ) Disorders are also thought to develop with the onset of the destructive process in the tissues as a result of this balance being disrupted.

Purpose: This study was designed to compare salivary and serum oxidative stress and inflammation markers of individuals with Temporomandibular Disorders (TMD) and healthy subjects.

Study design, setting, and sample: A prospective cross-sectional study was conducted. 27 TMD patients diagnosed with disc displacement (DD) according to RDC/TMD and 17 healthy subjects were enrolled in the study. Prior to any treatment, serum and saliva samples were taken from the patients and centrifuged, and stored at -80°C until analyzed. All samples were examined for IL-6, MDA and 8-OHdG concentrations.

Predictor variable: Diagnosis of disc displacement (RDC/TMD) Main outcome variables: Levels of 8-OHdG, IL-6 and MDA Covariates: Age and gender

Description:

The term oxidative stress is defined as any condition that causes free radical accumulation in tissue. Oxidative stress, which is an indicator of the deterioration of the balance between oxidant/antioxidant in an organism, is thought to play a role in the pathogenesis of various diseases such as atherosclerosis, aging, cancer, neurological disorders, diabetes, ischemia/reperfusion, Alzheimer's, Parkinson's, rheumatoid arthritis and chronic inflammation. Recently, many studies have shown a relationship between the pathogenesis of TMD and various free radicals, antioxidant enzymes and inflammation mediators. Through biomolecule methods studies determine the increase of some of cytokines such as Interleukin 1β (IL-1β), IL-6 in TMJ with internal derangement. These studies emphasize the positive relationship between the severity of the disease and the levels of synovial fluid mediators in joints with TMD. Although it has been supported by previous studies that synovial fluid is a reliable diagnostic material-that reflects the pathological condition in the joint, the disadvantages of the difficulty of obtaining has led to the need for the use of another realiable diagnostic tool. Saliva and blood are materials that are easily available and provide information about the formation and etiology of many systemic diseases.

The aim of this study is to compare the salivary and serum oxidative stress and inflammatory marker levels of TMD patients with RDC/TMD type II disorder with healthy subjects. For this purpose, IL-6, MDA and 8-OHdG will be evaluated as biomarkers of inflammation and oxidative stress.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 44
• Est. completion date: April 19, 2023
• Est. primary completion date: March 28, 2023
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 14 Years to 40 Years

Eligibility

Inclusion Criteria:

• TMD subjects who had RDC/TMD type II disorder (TMJ disc displacement (DD) with reduction

• TMJ-DD without reduction with limited opening

• TMJ-DD without reduction without limited opening)

Exclusion Criteria:

• other local or systemic diseases,

• pain of dental origin,

• pregnancy or lactation,

• story of the use of anti-inflammatory drugs, analgesics, muscle relaxants, vitamin C or vitamin E,

• smoking,

• individuals who had already been under treatment for TMD

Related Conditions & MeSH terms

• Inflammation
• Oxidative Stress
• Temporomandibular Disorder
• Temporomandibular Joint Disorders
• Temporomandibular Joint Dysfunction Syndrome

Intervention

Other:
• Biochemical analysis
Oxidative stress and inflammation markers were analyzed in serum and saliva of TMD and healthy subjects.

Locations

Country	Name	City	State
Turkey	Bahcesehir University	Istanbul	Besiktas

Sponsors (1)

Lead Sponsor	Collaborator
Bahçesehir University

Country where clinical trial is conducted

Turkey, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	relationship between serum and salivary 8-OHdG, IL-6 and MDA levels	relationship between serum and salivary 8-OHdG, IL-6 and MDA levels and Temporomandibular Disorders were evaluated	through study completion, an average of 1 year