Clinical Trial Details
— Status: Completed
Administrative data
NCT number |
NCT03194880 |
Other study ID # |
Alere COMBO & Alere q Detect |
Secondary ID |
|
Status |
Completed |
Phase |
|
First received |
|
Last updated |
|
Start date |
December 8, 2016 |
Est. completion date |
February 10, 2023 |
Study information
Verified date |
April 2022 |
Source |
Thai Red Cross AIDS Research Centre |
Contact |
n/a |
Is FDA regulated |
No |
Health authority |
|
Study type |
Observational
|
Clinical Trial Summary
Two new rapid HIV tests by Alere™ have the potential to substantially decrease the window
period for rapid tests. First, the Alere™ HIV Combo is a rapid fourth generation test. The
performance in a laboratory based setting was recently assessed at the Thai Red Cross
Anonymous Clinic Laboratory in Bangkok, Thailand (personal communication). Of 50 confirmed
acute HIV samples, Alere™ HIV Combo could detect 37 (74%), while none of these 50 cases could
be identified by the currently used third generation tests. These data have not yet been
published, but the preliminary results indicate a very favorable performance of the Alere™
HIV Combo in a facility-based laboratory setting.
Second, the Alere™ q HIV-1/2 Detect is a qualitative, cartridge based, nucleic acid
amplification test designed for Point of Care use. Data from several early infant diagnosis
studies in Sub-Saharan African countries have assessed the performance of the Alere™ q
HIV-1/2 Detect. The test was performed in the field, by a range of health professionals, from
nurses and laboratory technicians to medical doctors. The pooled analysis showed a
sensitivity of 99.07% (95%CI 95.48 - 99.95%) and a specificity of 99.94% (99.72-100%).
Description:
This will be a prospective study, collecting blood samples from individuals receiving PrEP at
one of two drop-in centers in Bangkok, Thailand:
Rainbow Sky Association of Thailand (RSAT) and Service Worker IN Group (SWING). RSAT is a
community-based organization for people with sexual diversity (MSM, gay, transgender,
lesbians, and bisexual), and has specific programs for MSM, transgender and lesbian health.
SWING promotes HIV prevention among male and female sex workers and provides both community
outreach and clinical services at their center. Both locations offer PrEP as part of their
service.
Participants who visit the DICs seeking to receive PrEP will undergo HIV testing at several
time points: before receiving PrEP (Month 0), one month after starting PrEP (Month 1), three
months after starting PrEP (Month 3), and every three months thereafter.
The routine HIV testing algorithm in the DIC is as follows:
- Determine® HIV-1/2 (Alere Medical Co., Ltd.), if reactive followed by two other rapid
3rd generation immunochromatography anti-HIV tests. These are all rapid tests on whole
blood as collected by finger prick, performed by trained community health workers in the
DIC.
For the purpose of this study, two additional blood samples will be taken by phlebotomy to
perform:
- The Anonymous Clinic HIV-testing algorithm (see Annex 1): Architect® HIV-1/2 Combo
(Abbott), if reactive followed by Determine® HIV-1/2 (Alere) and Elecsys® HIV combi PT
(Roche). Samples that are non-reactive by Architect® HIV-1/2 Combo will be tested by
pooled NAAT using Aptima HIV-1 RNA qualitative assay (Hologic®) to screen for acute HIV
infection. Samples that are positive by Architect®, Determine® and Elecsys® will be
tested by second generation EIA (which detects IgG Ab) to identify acute HIV infection
(i.e. 4th generation reactive and 2nd generation negative). This is the standard HIV
testing algorithm performed at the Thai Red Cross Anonymous Clinic Laboratory and will
identify acute HIV infections.
- Alere™ HIV Combo Alere™ q HIV-1/2 Detect Both the regular DIC algorithm and the
Anonymous Clinic algorithm will be used as comparator methods.