Adipose Tissue Blood Flow in Aging Humans

Healthy Clinical Trial

Official title:

Adipose Tissue Blood Flow in Aging Humans

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT06096532
• Other study ID #: 23-004200
• Secondary ID: UL1TR002377
• Status: Recruiting
• Phase: N/A
• Start date: January 15, 2024
• Est. completion date: August 2024

Study information

• Verified date: February 2024
• Source: Mayo Clinic
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The goal of this basic science clinical trial is to understand whether adipose tissue blood flow changes between younger and older healthy adults.

The main question[s] it aims to answer are:

• Is basal and nutrient responsive adipose tissue blood flow (ATBF) different between younger and older adults

• What molecular and systemic signatures related to adipose tissue blood flow are altered between these two groups.

Participants will undergo measurements of adipose tissue blood flow using the xenon washout technique, undergo 2 abdominal subcutaneous adipose tissue biopsies, and drink a sugary drink.

Description:

On Visit 1, volunteers will provide written informed consent (and urine pregnancy test for women of childbearing age), complete a Dual x-ray absorptiometry scan, baseline labs (comprehensive metabolic panel) and a cardiopulmonary exercise test. Participants will arrive fasted at 08:00, and urine pregnancy test for women of childbearing age administered and an intravenous catheter will be placed. Following 30min of supine rest, baseline venous blood samples will be collected. ATBF will be assessed on participants' left abdomen first by measurement of blood flow using 133Xenon washout method. Immediately thereafter, an abdominal adipose tissue biopsy of the left side will be performed but ≥6 cm distant from location of 133Xenon injections. Subjects will them consume 75 gm glucose; because ATBF peaks 30-60 minutes following ingestion of glucose, ATBF measures will start 30 minutes after ingestion on the contralateral side followed immediately by plasma measures and another adipose tissue biopsy. During ATBF measurements, brachial blood pressure will be assessed in two-minute intervals.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 24
• Est. completion date: August 2024
• Est. primary completion date: August 2024
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 80 Years

Eligibility

Inclusion criteria

• Within the ages of 18-35 years or 65-80 years at time of study visit

• BMI between 20-25.0 kg/m2.

Exclusion criteria:

• Presence of diagnosed cardiometabolic diseases (e.g., Type 2 diabetes, hypertension, Heart Failure)

• Taking prescription anticoagulants

• Taking prescriptions or supplements that effect adipose tissue metabolism (i.e., statins, Thiazolidinediones, niacin, atypical antipsychotics, or fish oil)

• Pregnant or nursing

• Inability to provide written informed consent

• Smokers

Related Conditions & MeSH terms

• Aging
• Healthy

Intervention

Other:
• 75 gm oral glucose load
Consumption of 75 gm oral glucose load in order to test nutrient responsiveness

Locations

Country	Name	City	State
United States	Mayo Clinic Minnesota	Rochester	Minnesota

Sponsors (2)

Lead Sponsor	Collaborator
Mayo Clinic	National Center for Advancing Translational Sciences (NCATS)

Country where clinical trial is conducted

United States, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Adipose tissue blood flow	blood flow will be measured in the upper body subcutaneous adipose tissue using 133 Xenon washout and reported as blood flow in ml/min/100g adipose tissue.	Baseline, pre-intervention fasting and immediately following 75 gm oral glucose load
Secondary	Genomic alterations	RNA-Seq from adipose tissue biopsies	Baseline, pre-intervention fasting and immediately following 75 gm oral glucose load