Molecular and Hormonal Responses to Diet-Induced Insulin Resistance

Healthy Clinical Trial

Official title:

Molecular and Hormonal Responses to Diet-Induced Insulin Resistance

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT03879187
• Other study ID #: R13-P171
• Status: Completed
• Phase: N/A
• Start date: March 2, 2015
• Est. completion date: May 22, 2018

Study information

• Verified date: March 2019
• Source: Loughborough University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This study determined the effect of 7 days of high-fat overfeeding on whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function in 15 healthy young individuals.

Description:

Fifteen healthy, young individuals underwent metabolic testing before and after 7 days of high-fat overfeeding. We determined whole-body glycaemic control, glucose kinetics, skeletal muscle insulin signalling, and markers of skeletal muscle microvascular function. Stable isotope tracers were used to assess the rate of appearance of glucose from the gut, liver glucose output and whole-body glucose disposal. Insulin signalling was determined by Western blotting of skeletal muscle tissue. Microvascular function was assessed through the use of quantitative immunofluorescence microscopy. These measurements were performed both postabsorptively and following the ingestion of 50 grams of glucose + 15 grams of protein.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 15
• Est. completion date: May 22, 2018
• Est. primary completion date: May 22, 2018
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years to 40 Years

Eligibility

Inclusion Criteria:

• Aged between 18 and 40 years

• Physically active

• Non-smoker

• Not taking any medication known to interfere with the study

• No history of cardiovascular disease or diabetes

• Weight stable for the last 6 months

Exclusion Criteria:

• Outside of age range

• Sedentary

• Smoker

• Taking medication known to interfere with the study

• History of cardiovascular disease or diabetes

• Unstable weight within last 6 months

Related Conditions & MeSH terms

• Healthy
• Insulin Resistance

Intervention

Dietary Supplement:
• High-fat, high-energy diet
Participants consumed a diet that was high in fat and high in energy

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Loughborough University

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Change in glucose concentration	Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Glucose was reported as mmol/L.	Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Primary	Change in insulin concentration	Venous blood samples were obtained the day before and the day after the 7 day high-fat diet. Insulin was reported as pmol/L.	Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Primary	Change in glucose kinetics (i.e., rate of appearance and disappearance) assessed using isotope tracers	Glucose kinetics were assessed the day before and the day after the 7 day high-fat diet. Data expressed as umol/kg/min.	Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Primary	Change in skeletal muscle insulin signalling	AKT and AS160 phosphorylation was determined by Western blotting of skeletal muscle. Data are expressed as arbitrary units.	Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Primary	Change in markers of microvascular function	endothelial nitric oxide synthase was determined in terminal arterioles by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units.	Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)
Secondary	Change intramuscular triglyceride content	Intramuscular triglyceride content was determined by quantitative immunofluorescence microscopy that was performed on sections of skeletal muscle tissue. Data presented as arbitrary units.	Immediately before and after the 7 day high-fat diet (i.e., measurements were made 7 days apart)