LXR and Oocyte Meiosis Resumption

Female Infertility Clinical Trial

Official title:

Are LXRs Involved in Regulation of Human Oocyte Meiosis Resumption?

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT03045315
• Other study ID #: CHU-303
• Status: Recruiting
• Phase: N/A
• First received: January 27, 2017
• Last updated: February 7, 2017
• Start date: January 2015
• Est. completion date: August 2019

Study information

• Verified date: February 2017
• Source: University Hospital, Clermont-Ferrand
• Contact: Patrick LACARIN
• Phone: 04 73 75 11 95
• Email: placarin[@]chu-clermontferrand.fr
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Previous studies in animal model showed clearly that lipid homeostasis influence oocyte meiosis resumption. However Liver X Receptors pathway has never been investigated in human ovocyte

Description:

In mice deficient of Liver X Receptor (LXR) genes, the oocytes are unable to resume meiosis. Oxysterols are molecules derived from the cholesterol synthesis pathway. They are ligands of the liver X receptors (LXRs). In human, the LXR signalling pathway has never been investigated in this process. The Investigator propose to analyse in granulosa cells surrounding ovocyte the expression of LXR, their target genes and the enzymes involved in oxysterols synthesis. These granulosa cells are normally retrieved and destroyed 18 hours after sperm insemination of oocytes in a In Vitro Fertilization program. The oocyte meiosis resumption will be considered as positive (Granulosa cell positive, GC+) if a zygote is observed 18 hours after sperm insemination and negative (Granulosa cell negative, GC-) if none zygote appears. Moreover the investigator will analyse the quality of embryo development during 5 days after zygote is formed (normal process of In Vitro Fertilization) . The both populations of granulosa cells (GC+ and GC-) will be compared. The measurements of Liver X Receptor genes and the enzymes involved in oxysterols pathways xill be performed by Reverse Transcription-Polymerase Chain Reaction. The accumulation of lipid in granulosa cells will be measures by oil red staining. By this sudy the investigator will probably find new biological markers of resumption meiosis for women enrolled in In Vitro Fertilization programm. Moreover, data will help to better understand the physiopathology of oocyte meiosis failures.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 250
• Est. completion date: August 2019
• Est. primary completion date: May 2019
• Accepts healthy volunteers: No
• Gender: Female
• Age group: 18 Years to 36 Years

Eligibility

Inclusion Criteria:

• woman aged less 37 years

• included in a IVF program

• tubal infertility

• ovarian stimulation with recombinant FSH

• induced ovulation by recombinant chorionic gonaotrophin

Exclusion Criteria:

• dysovulation

• male infertility

• Ovarian insuficiency

Related Conditions & MeSH terms

• Female Infertility
• Infertility
• Infertility, Female

Intervention

Other:
• IVF Procedure

Locations

Country	Name	City	State
France	CHU Clermont-Ferrand	Clermont-Ferrand

Sponsors (1)

Lead Sponsor	Collaborator
University Hospital, Clermont-Ferrand

Country where clinical trial is conducted

France, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Expression of RNAs of LXR pathways in granulosa cells surrounding oocyte	The day of the retrieve of granulosa cells, RNA extraction will be performed and the expression of Liver X Receptors genes, their downstream target genes and the enzymes of oxyterols synthesis will be analysed.	at day 1
Secondary	Accumulation of lipid in granulosa cells surrounding oocyte	Embryo development of inseminated ovocytes at day 5 after sperm insemination during In Vitro Fertilization process	at day 5