Nanopore Sequencing in Ophthalmology

Status Recruiting

Clinical Trial Summary

In this pilot study, the feasibility of the Oxford Nanopore MinION to identify pathogens from vitrectomy samples in eyes with endophthalmitis shall be assessed. The MinION is a low cost commercially available device for DNA/ RNA analysis that, in studies, has been used for pathogen determination in various infectious diseases as well as for the genetic characterization of hematologic tumors.

Clinical Trial Description

Vitreous samples and anterior chamber taps from eyes with endophthalmitis are taken during routine vitrectomy. The gathered samples are divided in half to undergo routine culture for microbial growth and nanopore sequencing with the MinION Mk1b, a commercially available device for DNA/RNA sequencing. Base reads from Nanopore sequencing are then compared with available libraries for pathogen identification. The Nanopore sequencing results for pathogens are compared versus the results of the microbiological culture of the same eye as the gold standard. Clinical features and visual function before and after vitrectomy are gathered from patient charts. Treatment is not altered due to results of the Nanopore sequencing. ;

Study Design

Related Conditions & MeSH terms

Endophthalmitis

Clinical Trial Details

NCT number	NCT05372861
Study type	Observational
Source	Medical University of Graz
Contact	Michael Sommer, MD
Phone	004331638531033
Email	michael.sommer@medunigraz.at
Status	Recruiting
Phase
Start date	April 22, 2022
Completion date	December 1, 2024

View Details

See also
Status	Clinical Trial	Phase
Recruiting	`NCT04214821` - The Pharmacokinetics of Topical Levofloxacin 1.5% vs Topical Moxifloxacin 0.5%	Phase 1/Phase 2
Completed	`NCT00461656` - Povidone-iodine Antisepsis for Strabismus Surgery	Phase 4
Completed	`NCT00136344` - Study of Antibiotic Prophylaxis for Endophthalmitis Following Cataract Surgery	N/A
Completed	`NCT04568213` - Hypochlorous Acid Disinfection Prior to Cataract Surgery	Phase 3
Terminated	`NCT01496794` - Endophthalmitis Cultures	N/A
Completed	`NCT00406913` - Prophylaxis Prior to Cataract Surgery Conjunctival Flora and Optimal Ocular Sterilization Technique Prior to Cataract Surgery	N/A
Recruiting	`NCT05079854` - Use Of Intracameral Moxifloxacin For Prophylaxis Of Open Globe Endophthalmitis	Phase 4
Recruiting	`NCT05432336` - Complex Ocular Infection, Optimization of Microbiological Diagnosis
Recruiting	`NCT02810587` - Endophthalmitis After Intravitreous Anti-VEGF Injections in Patients Receiving vs. Not Receiving Topical Antibiotics	N/A
Completed	`NCT00398658` - Prophylaxis of Acute Post-Traumatic Bacterial Endophthalmitis	Phase 4
Recruiting	`NCT04035369` - Endophthalmitis Post Intravitreal Injections	N/A
Not yet recruiting	`NCT03420820` - Concentrating on Antisepsis: 5% vs. 10% Povidone-Iodine Prior to Intravitreal Injection	N/A
Recruiting	`NCT06268561` - Ozurdex Endophtamitis Cohort, Prognostic Assessment at 12 Months
Withdrawn	`NCT00335231` - Preoperative Topical Gatifloxacin on Anterior Chamber Cultures After Cataract Surgery	N/A
Not yet recruiting	`NCT03244072` - Safety and Efficacy of Intracameral Zimoxin for Prevention of Endophthalmitis After Cataract Surgery	Phase 2/Phase 3
Completed	`NCT01323868` - Endogenous Endophthalmitis in the Inpatient Setting	N/A
Completed	`NCT03363295` - Evaluation of Macular Changes After Intracameral Moxifloxacin for Prevention of Endophthalmitis	Phase 4
Suspended	`NCT02590523` - Intracameral Antibiotic Safety Study	Phase 3
Terminated	`NCT02770729` - Evaluation of Efficacy and Safety of Intracameral Moxifloxacin for Prevention of Postcataract Endophthalmitis.	Phase 3
Not yet recruiting	`NCT02324166` - Cefazolin-Lidocaine Combination Solution to Reduce Antibiotic Pain	Phase 4