Salivary TAS, TOS, LL-37 and Dental Status in Passive Smoking Children

Dental Caries Clinical Trial

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04292548
• Other study ID #: Kirikkale pediatric dentistry
• Status: Completed
• Start date: November 16, 2016
• Est. completion date: August 15, 2018

Study information

• Verified date: February 2020
• Source: Kirikkale University
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

The aim of the present study was to determine the effect of passive smoking on the levels of salivary TAS, TOS, LL-37 and dental status in children.In the study the parents of children were asked to fill out a complete survey about smoking habits. After filling out the questionnaire, according to the survey results, a total of 180 children were included to the study as follows; 90 children exposed to passive smoking, and 90 children in the control group (unexposed controls). Also demographic data were recorded (age, gender, parental education levels, child's tooth-brushing habit and child's daily dietary sugar exposure, family income). Dental examination of children were performed and caries prevalance of the patients were recorded. Unstimulated saliva samples were collected from children. Saliva 'cotinine levels' which are expected to increase in passive smoking group; 'antimicrobial peptide LL-37' and oxidative stress markers 'total antioxidant status' (TAS), and' total oxidant status' (TOS) were evaluated by using Enzyme-linked immunosorbent assay (ELISA). The obtained data parameters of the two groups were evaluated and comparison was performed.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 180
• Est. completion date: August 15, 2018
• Est. primary completion date: April 15, 2018
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 6 Years to 12 Years

Eligibility

Inclusion Criteria:

-Systemically healthy children

Exclusion Criteria:

• The presence of orthodontic / intraoral appliance

• Any disease or condition known to affect defense system

• Uncooperative children

Related Conditions & MeSH terms

• Children
• Dental Caries
• Oxidative Stress
• Passive Smoking

Intervention

Diagnostic Test:
• taking saliva samples to determine TAS, TOS and LL-37

Locations

Country	Name	City	State
n/a

Sponsors (2)

Lead Sponsor	Collaborator
Merve Erkmen Almaz	Kirikkale University

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	salivary oxidative stress values: TAS level	Unstimulated saliva samples were collected from children.	1 day (once after the dental examination)
Primary	salivary oxidative stress values: TOS level	Unstimulated saliva samples were collected from children.	1 day (once after the dental examination)
Primary	salivary LL-37 level	Unstimulated saliva samples were collected from children.	1 day (once after the dental examination)
Primary	salivary cotinine levels	Unstimulated saliva samples were collected from children for describing passive smoking children.	1 day (once after the dental examination)