Vulvodynia Clinical Trial
Official title:
A Laboratory and Clinical Search for the Presence of H. Pylori and Treatment Against it in Localized Vulvodynia (Vestibulitis, Vestibulodynia).
Hypothesis: to examine a possible association between localized vulvodynia and H. pylori infection.
Subsequent sections from the paraffin blocks were prepared and stained by modified Giemsa.
Immunostaining for H. pylori was done as described. In short, tissue sections were
deparaffinized in xylene, rehydrated through decreasing concentrations of alcohol ending in
phosphate-buffered saline (PBS), and subjected to pretreatment with Proteinase K (8
minutes). The sections were quenched with 3% hydrogen peroxidase, incubated with protein
block for 15 minutes at room temperature, and washed in PBS. Tissues then were incubated
with polyclonal rabbit anti-H pylori antibody (dilution, 1:10; clone ch-20 429, DAKO,
Carpinteria, CA). Finally, sections were washed in 0.05% polysorbate 20 in PBS, pH 7.4, and
the bound antibody was detected using streptavidin and biotinylated secondary antibody with
diaminobenzidine as the chromogen. Sections were counterstained with hematoxylin,
dehydrated, and mounted. Negative controls were sections treated as above, but instead of
incubation with the primary antibody, they were incubated with 1% bovine serum albumin in
PBS.
Vulvar biopsies of seven other women without localized vulvodynia served as healthy
controls.
The positive and negative control gastric tissues for the immunohistochemical stain of the
H. pylori microorganisms were obtained from the archives of the Department of Pathology.
;
Observational Model: Case Control, Time Perspective: Cross-Sectional
Status | Clinical Trial | Phase | |
---|---|---|---|
Completed |
NCT05478746 -
Effects of Flourish HEC on Localized Provoked Vulvodynia
|
N/A | |
Completed |
NCT02404961 -
Women's Health Study: Immunological Factors and Risk of Vulvodynia
|
||
Completed |
NCT00751010 -
Relationship: Interstitial Cystitis & Vulvodynia-Part 2
|
N/A | |
Completed |
NCT00607490 -
A Randomized Clinical Trial for Women With Vulvodynia
|
N/A | |
Recruiting |
NCT03640624 -
Multidisciplinary Treatment of Chronic Vulvar Pain
|
N/A | |
Recruiting |
NCT05343182 -
Vestibulectomy Surgical Techniques Comparison Study
|
N/A | |
Recruiting |
NCT05518630 -
An Investigation of Nomothetic Versus Idiographic Assessment in Chronic Pain
|
||
Recruiting |
NCT05597358 -
Efficacy of High Intensity Laser for Provoked Vestibulodynia
|
N/A | |
Completed |
NCT01304589 -
Savella in Treatment for Provoked Vestibulodynia
|
Phase 3 | |
Completed |
NCT00590590 -
Safety and Efficacy of Two Vaginal Products Versus Placebo in Patients With Vaginal Discomfort
|
Phase 2 | |
Recruiting |
NCT06138171 -
Personality, Defences, Central Sensitization, and Trauma in Women With Chronic Migraine, Fibromyalgia, and Vulvodynia
|
||
Completed |
NCT01996384 -
Provoked, Localized Vulvodynia Treatment With Acupuncture and Lidocaine Pilot Study
|
Phase 1 | |
Completed |
NCT01664962 -
Search for Genetic Basis of Vulvodynia
|
N/A | |
Recruiting |
NCT05955313 -
Effectiveness of Low-dose Naltrexone in Patients With Different Types of Vulvodynia
|
Phase 2 | |
Completed |
NCT02773641 -
Placebo-controlled RCT of Botulinum Toxin A as a Treatment for Provoked Vestibulodynia
|
Phase 3 | |
Completed |
NCT04057755 -
Botulinum Toxin A as a Treatment for Provoked Vestibulodynia
|
Phase 3 | |
Terminated |
NCT04016467 -
Effect of Spinal Manipulation on Vulvar Pain
|
N/A | |
Completed |
NCT01935063 -
Study to Compare the Efficacy of Cognitive-behavioral Couple Therapy and Lidocaine for Provoked Vestibulodynia
|
N/A | |
Completed |
NCT02809612 -
An Internet-based Information Platform for Vulvodynia Patients
|
N/A | |
Not yet recruiting |
NCT02393911 -
Dysbiosis in Localized Provoked Vulvodynia (LPV)
|
Phase 3 |