Microarchitecture of Vitreoretinal Contact

Vitreo-Retinal Adhesion Clinical Trial

Official title:

Microarchitecture of Vitreoretinal Contact

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04802889
• Other study ID #: 124576
• Status: Completed
• Phase: N/A
• Start date: January 1, 2008
• Est. completion date: December 31, 2020

Study information

• Verified date: March 2021
• Source: The S.N. Fyodorov Eye Microsurgery State Institution
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

Investigation of the vitreoretinal contact microarchitecture in the macular and paramacular zones of the human retina.

Description:

Purpose: to study the features of the macular ILM, to study the features of the preretinal structures of the vitreous body of the Central retinal region, morphofunctional features of preretinal hyalocytes, and the mechanism of vitreoretinal adhesion.

Study Protocol: examination of donor human eyes obtained from an eye Bank after sampling material for corneal transplantation.

Research method: scanning electron microscopy (SEM) of the central retina (macular and paramacular zone): ILM, preretinal vitreous.

Scientific hypothesis: vitreoretinal adhesion is provided not by laminin and fibronectin, but by the interweaving of vitreous collagen fibers between the epiretinal vitreous (more firmly fixed to the retinal surface) and the cortical vitreous.

Expected results: determination of ILM thickness in the macular zone, microanatomy of preretinal vitreous strutcures, and possible mechanism of vitreoretinal adhesion, establishing the possibility of intravital synthesis of collagen fibers synthesis in the adult vitreous.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 20
• Est. completion date: December 31, 2020
• Est. primary completion date: January 1, 2020
• Accepts healthy volunteers: No
• Gender: All
• Age group: 35 Years to 45 Years

Eligibility

Inclusion Criteria:

• eyes from donors without concomitant multiple organ pathology and ophthalmic pathology

Exclusion Criteria:

• eye injury, age over 45 years, concomitant multiple organ pathology, previous eye diseases

Related Conditions & MeSH terms

• Tissue Adhesions
• Vitreo-Retinal Adhesion
• Vitreoretinal Detachment

Intervention

Other:
• scanning electron microscopy
scanning electron microscopy of the ?entral retina and preretinal structures

Locations

Country	Name	City	State
Russian Federation	S.N. Fedorov Nmrc "Mntk "Eye Microsurgery"	Moscow

Sponsors (1)

Lead Sponsor	Collaborator
The S.N. Fyodorov Eye Microsurgery State Institution

Country where clinical trial is conducted

Russian Federation, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	the ILM thickness in the macular zone (foveola)	the ILM thickness in the macular zone (foveola) in mkm by scanning electron microscopy data	within 3 days after receiving the cadaver eyes
Primary	superficial microstructure of ILM	by scanning electron microscopy data	within 3 days after receiving the cadaver eyes
Primary	features of the structural microarchitecture of the preretinal vitreous in the macular zone	by scanning electron microscopy data	within 3 days after receiving the cadaver eyes
Primary	morphofunctional features of preretinal hyalocytes.	by scanning electron microscopy data	within 3 days after receiving the cadaver eyes

External Links

•   Antithesis to the thesis about fibronectin and laminin as the main components providing vitreoretinal adhesion (article)
•   Cellular Microanatomy of Vitreoretinal Interface. Morphofunctional Identification of Epiretinal Vitreous Cells. Collagen Synthesis in Adult Human Vitreous (article)
•   Microanatomy of Internal Limiting Membrane (article)
•   Microanatomy of vitreoretinal interactions in macular and paramacular areas (article DOI: 10.17816/KMJ2019-595)
•   New data of mechanism in vitreoretinal adhesion (Euretina2020 abstracts)
•   New Data of Mechanism in Vitreoretinal Adhesion and Posterior Vitreous Detachment in Humans (article)