Antibacterial Activity of Urea Against Ocular Bacteria

Urea in Blood; High Clinical Trial

Official title:

In Vitro Evaluation of Antibacterial Activity of Urea Solution Against Ocular Bacterial Isolates

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04020406
• Other study ID #: 2323297
• Status: Completed
• Start date: November 1, 2017
• Est. completion date: November 1, 2018

Study information

• Verified date: July 2019
• Source: Assiut University
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

Antibacterial properties of urea was tested against 35 isolates from ocular infections.

Description:

In vitro susceptibility testing was performed with commercially available antibiotic discs, homemade urea discs saturated with urea solution at a concentration of 96 mmol. The weight of urea in one drop = 0.37 mg, in one ml = 10.34 mg (5 microliter/ disc) discs and combined discs (antibiotic discs to which urea solution was added). Combined discs provided better in vitro susceptibility results than antibiotics alone.

Scanning electron micrographs of bacterial isolates incubated overnight with urea solution showed marked disruption of bacterial aggregative patterns and irregularities of their cell walls.

Western blotting showed marked reduction in total proteins in bacterial isolates incubated overnight with urea solution. These findings may explain the possible mechanisms for antibacterial activity of urea solution as a result of protein denaturation.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 90
• Est. completion date: November 1, 2018
• Est. primary completion date: November 1, 2018
• Accepts healthy volunteers: No
• Gender: All
• Age group: 5 Months to 65 Years

Eligibility

Inclusion Criteria:

• ocular infection

Exclusion Criteria:

• Local antibiotics treatment

Related Conditions & MeSH terms

• Urea in Blood; High
• Uremia

Intervention

Biological:
• Antibacterial Activity
Antibacterial Activity of Urea Solution against Ocular Bacterial Isolates

Locations

Country	Name	City	State
Egypt	Hassan Lotfy Fahmy	Assiut

Sponsors (1)

Lead Sponsor	Collaborator
Assiut University

Country where clinical trial is conducted

Egypt, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Antibacterial effect of Urea Solution against Ocular Bacterial	Samples were both inoculated on Muller Agar (Sigma-Aldrich) plates and directly examined under the microscope using Gram stained smears. Inoculated plates were incubated at 37oC for 48 hrs.; Gram negative isolates were sub-cultured on MacConkey's, Eosin Methelyene Blue (EMB) and Cystine Lactose Electrolyte Deficient (CLED) agar plates (Sigma-Aldrich). Gram positive isolates were sub-cultured on Mannitol Salt agar plates (Sigma-Aldrich).	6 month