Telomeric Abnormalities in Colorectal Diseases by Fluorescent in Situ Hybridization Technique

Tumor Gastric Clinical Trial

Official title:

Telomeric Abnormalities in Benign and Malignant Colorectal Diseases by Fluorescent in Situ Hybridization Technique

Clinical Trial Details — Status: Not yet recruiting

Administrative data

• NCT number: NCT03208777
• Other study ID #: FISH
• Status: Not yet recruiting
• Phase: N/A
• First received: July 3, 2017
• Last updated: July 5, 2017
• Start date: August 1, 2017
• Est. completion date: August 2019

Study information

• Verified date: July 2017
• Source: Assiut University
• Contact: eman mosaad, prof.dr
• Phone: 00201065518821
• Email: Eman_mosaad[@]hotmail.com
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

Colorectal carcinoma is a heterogeneous disease that is caused by the interaction of genetic and environmental factors. colorectal carcinoma encompasses a complex disease with different molecular pathways and biological characteristics arising from a multi-step process that implicates several genetic and epigenetic events . The multi-step genetic model involves the loss of function of tumor suppressor genes, such as adenomatous polyposis coli (APC), Telomeres could be a promising marker due to the fact that their lengths change in the colorectal polyp-carcinoma sequence . Moreover, telomere length (TL) is altered in blood cells in patients with colorectal carcinoma

• These findings could suggest that changes in TL may take place before the development of the tumor .

The two main forms of inflammatory bowel disease (IBD), ulcerative colitis (UC) and Crohn's disease (CD) are characterized by chronic intestinal inflammation and risk of progression to colon cancer. One proposed cause of the latter characteristic is chromosome instability, since the rearrangement of genetic material can lead to activation of oncogenes, loss of tumor suppressor genes and other changes that lead to uncontrolled cell growth. Chromosome instability is particularly associated with UC and has been observed in colon epithelial cells and peripheral blood mononuclear cell. Since genomic instability in peripheral blood mononuclear cells (PBMCs) has been used as a biomarker for global cancer risk in a number of diseases, the latter observation suggests the possibility of a chromosome instability syndrome in UC that could affect all tissues. One possible cause of chromosome instability is telomere dysfunction .

Description:

Human chromosomes are capped and stabilized by telomeres, which not only protect them from damage but also have a role in regulating cellular senescence. After reaching a critical length, telomeres experience a double DNA change and cells will eventually enter senescence (replication) or cell death . Telomere length and telomere shortening have been long hypothesized to be a biological marker of aging at the cellular level and a potential mechanism of carcinogenesis. Genomic instability is a critical factor in the initiation and progression of human cancers. One mechanism that underlies genomic instability is loss of telomere function .

fluorescent in situ hybridization is a molecular diagnostic technique that utilizes labeled DNA probes to detect or confirm gene or chromosome abnormalities. fluorescent in situ hybridization is often utilized for both research and diagnosis of hematological malignancies and solid tumors. Conceptually, fluorescent in situ hybridization is a very straightforward technique whereby a DNA probe is hybridized to its complementary sequence on chromosomal preparations previously fixed on microscope slides . fluorescent in situ hybridization is able to detect cells that have chromosomal abnormalities consistent with neoplasia .

There has been a surge of published studies which assessed the association between telomere length and development of colorectal carcinoma. Thus, a meta-analysis addressing colorectal carcinoma and telomere length would be a useful addition to the current information in this area.

Recruitment information / eligibility

• Status: Not yet recruiting
• Enrollment: 80
• Est. completion date: August 2019
• Est. primary completion date: August 1, 2018
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 18 Years and older

Eligibility

Inclusion Criteria:

• Adult age group ? 18 years.

• Newly diagnosed cases (no previous treatment).

• No treatment was taken for HCV infection.

Exclusion Criteria:

• age group < 18 years.

• Patients with malignancy of other type.

• Patients not diagnosed by endoscopy or biopsy (not surely diagnosed).

Related Conditions & MeSH terms

• Stomach Neoplasms
• Tumor Gastric

Intervention

Genetic:
• taking blood samples
taking blood samples and measure telomeric abnormalities

Locations

Country	Name	City	State
n/a

Sponsors (1)

Lead Sponsor	Collaborator
Assiut University

References & Publications (1)

Bosman F, Yan P. Molecular pathology of colorectal cancer. Pol J Pathol. 2014 Dec;65(4):257-66. Review. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	presence of telomeric abnormalities	measure percentage of telomeric abnormalities in benign and malignant colorectal diseases	one year