OC and ICTP in Gingival Crevicular Fluid During Piezocision Accelerated Orthodontic Treatment

Tooth Movement Clinical Trial

Official title:

Osteocalcin and Cross-Linked C-Terminal Telopeptides of Type I Collagen in Gingival Crevicular Fluid During Piezocision Accelerated Orthodontic Tooth Movement

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT04611984
• Other study ID #: 04.03.2013.38
• Status: Completed
• Phase: N/A
• Start date: March 2013
• Est. completion date: August 2014

Study information

• Verified date: October 2020
• Source: Marmara University
• Contact: n/a
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

The aim of this study was to evaluate the tooth movement with and without piezocision with regard to the levels of osteocalcin and cross-linked C-terminal telopeptides of type I collagen in the gingival crevicular fluid.

Description:

15 systemically healthy subjects requiring extraction of the maxillary 1. premolars with distalization of the maxillary canines were enrolled to the study. Piezocisions were performed on the distal and mesial sides of the right maxillary canines while the left maxillary canines served as controls. Canine distalization was performed via closed-coil springs applying 150 g of force per side by using miniscrews as anchorage. Gingival crevicular fluid samples were collected from both mesial and distal sides of right and left maxillary canines at 0, 1, 7, 14 and 28 days. The gingival crevicular fluid levels of osteocalcin and cross-linked C-terminal telopeptides of type I collagen were determined by using ELISA. The rate of tooth movement was measured on 14 and 28 days.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 15
• Est. completion date: August 2014
• Est. primary completion date: August 2014
• Accepts healthy volunteers: Accepts Healthy Volunteers
• Gender: All
• Age group: 13 Years to 20 Years

Eligibility

Inclusion Criteria:

• Class I, Class II or Class III malocclusion,
• Proclined incisors requiring extraction of the maxillary first premolars with subsequent retraction of the maxillary canines
• No previous orthodontic treatment
• Good oral hygiene and periodontal health (plaque and gingival indices less or equal 1, probing depth less or equal 3 mm, no radiographic bone loss).

Exclusion Criteria:

• having systemic diseases that could affect bone and tooth movement
• smoking

Related Conditions & MeSH terms

• Tooth Movement

Intervention

Procedure:
• Piezocision
Piezocisions were performed on the mesial and distal sides of the right maxillary canines. Then canine distalization was performed via closed-coil springs applying 150 g of force by using mini-screws as anchorage.
• Control
The mesial and distal sides of the left maxillary canines served as controls and canine distalization was performed via closed-coil springs applying 150 g of force by using mini-screws as anchorage.

Locations

Country	Name	City	State
Turkey	Marmara University Faculty of Dentistry	Istanbul

Sponsors (1)

Lead Sponsor	Collaborator
Marmara University

Country where clinical trial is conducted

Turkey, 

References & Publications (3)

Alfaqeeh SA, Anil S. Osteocalcin and N-telopeptides of type I collagen marker levels in gingival crevicular fluid during different stages of orthodontic tooth movement. Am J Orthod Dentofacial Orthop. 2011 Jun;139(6):e553-9. doi: 10.1016/j.ajodo.2011.03.005. — View Citation

Dibart S, Surmenian J, Sebaoun JD, Montesani L. Rapid treatment of Class II malocclusion with piezocision: two case reports. Int J Periodontics Restorative Dent. 2010 Oct;30(5):487-93. — View Citation

Raj SC, Praharaj K, Barik AK, Patnaik K, Mahapatra A, Mohanty D, Katti N, Mishra D, Panda SM. Retraction With and Without Piezocision-Facilitated Orthodontics: A Randomized Controlled Trial. Int J Periodontics Restorative Dent. 2020 Jan/Feb;40(1):e19-e26. doi: 10.11607/prd.3968. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Gingival crevicular fluid osteocalcin level (ng/ml)	Gingival crevicular fluid samples were collected from mesial and distal sides of the maxillary right and left canines.The gingival crevicular fluid level of osteocalcin was determined by using ELISA	28 days after canine distalization
Primary	Gingival crevicular fluid cross-linked C-terminal telopeptides of type I level (ng/ml)	Gingival crevicular fluid samples were collected from mesial and distal sides of the maxillary right and left canines.The gingival crevicular fluid level of cross-linked C-terminal telopeptides of type I collagen was determined by using ELISA.	28 days after canine distalization
Secondary	The amount of canine movement (mm)	All measurements were performed on the dental cast model using a digital caliper. The amount of space closure was based on the measurements of the distance between the contact points of the distal surface of canine and the mesial surface of second premolar. The change of distance between the three observation periods represented the amount of canine movement.	Baseline (day 0) before canine distalization and, 14 and 28 days after canine distalization
Secondary	Retraction rate (mm/day) of canine movement	All measurements were performed on the dental cast model using a digital caliper. The amount of space closure was based on the measurements of the distance between the contact points of the distal surface of canine and the mesial surface of second premolar. The change of distance between the three observation periods represented the amount of canine movement, which was divided by the number of days between two sessions to obtain the retraction rate on daily basis	14 and 28 days after canine distalization