Sub-optimal Culture Conditions Clinical Trial
Official title:
The Blastocyst Development and Clinical Outcome in Single Step Medium With or Without Renewal on Day 3 in a Time-lapse Incubator
Culture media used to support development of zygotes to the blastocyst stage is based on two
basic philosophies: a single step medium "let-the-embryo-choose" or a sequential medium
"back-to-nature".
For single step media, there are two types of protocol can be used for the culture of human
preimplantation embryos:
1. Interrupted single medium culture, in which the medium is renewed on the Day 3 of
embryo culture
2. Uninterrupted single embryo culture, in which the same single medium is used throughout
the 5-6 days of culture with no replenishment or replacement on Day 3.
Investigators aim to compare the blastocyst development and clinical outcome between the two
types of protocols of a single medium (with or without renewal on Day 3)
n/a
Allocation: Randomized, Endpoint Classification: Efficacy Study, Intervention Model: Parallel Assignment, Masking: Double Blind (Investigator, Outcomes Assessor)