Topical Vancomycin Over Sternal Edge in Cardiac Surgery

Sternal Wound Infection Clinical Trial

Official title:

Topical Vancomycin Paste Over Sternal Edge: Safety and Effect of on Incidence of Sternal Wound Infection After Cardiac Surgery

Clinical Trial Details — Status: Recruiting

Administrative data

• NCT number: NCT04500275
• Other study ID #: A-BR-109-013
• Status: Recruiting
• Phase: Phase 4
• Start date: July 1, 2020
• Est. completion date: December 31, 2024

Study information

• Verified date: March 2024
• Source: National Cheng-Kung University Hospital
• Contact: MENG-TA TSAI, MD
• Phone: +886-2353535
• Email: dongsar[@]gmail.com
• Is FDA regulated: No
• Study type: Interventional

Clinical Trial Summary

This is a single-blind 1:1 randomized controlled trial based on the hypothesis that topical application of vancomycin paste over sternum edge is safe and can reduce sternal wound infection after elective cardiac surgery. Vancomycin paste will be prepared using 2.5 g of vancomycin mixed with 2 ml normal saline. Vancomycin paste as control or 2 ml normal saline as placebo will be spread on sternal edge immediately after sternotomy and before sternal closure. The safety of Vancomycin paste over sternum edge will be assessed according to postoperative serum Vancomycin exposure and potential side effects such as renal toxicity or bacterial resistance. Effect of topical Vancomycin on incidence of postoperative sternal wound infection will be assessed on postoperative 7, 30, and 90 days.

Description:

1. Patient Recruitment and Group Assignment

1. Institution. All the surgical procedures will be undertaken in the National Cheng Kung University Hospital (NCKUH) under service of the Cardiovascular Surgery Department. The patient monitoring will be carried out perioperatively in the general ward and intensive surgical unit (ICU, surgical or cardiac) of National Cheng Kung University Hospital.

2. Eligibility Criteria. Described in other section.

3. Randomization. Eligible patients will be 1:1 randomized using a computer-generated list to receive either normal saline (Control group) or Vancomycin Paste (Vancomycin group). A randomization list with corresponding patient recruitment number will be archived in NCKUH pharmacy. On the operating day, our research coordinator will order a study prescription, which looks identical to either control or Vancomycin group. The pharmacy will deliver the prescription content according to the patient recruitment number on the randomization list. The surgeons will know the patient grouping only after they get the prescription content in the operating room.

4. Blinding. The intraoperative staff including the surgeon will know the application material for the patients. The patient and the nursing staff in charge of sternal wound evaluation will not know the allocation of patients.

5. Sample Size Determination. The average incidence of DSWI after isolated CABG in NCKUH is 3-4% in recent 5 years. An expected reduction of DSWI from 4% to 1% leads to an effect size of 0.15, which will require a total sample size of 330 to achieve a statistical power of 0.8 given an alpha error probability of 0.05 and 1 degree of freedom. (Estimation with G*Power software). Considering the potential patient withdrawals, our planned sample collection will be 360 cases (180 in each group) in two years.

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2. Intervention

(1) Vancomycin group protocol. i. Timing of application: the Vancomycin® (China Chemical & Pharmaceutical Co., Ltd. (CCPC / Taiwab) paste will be spread on sternal edge immediately after sternotomy and before sternal closure.

ii. Regimen: The vancomycin paste will be prepared using 2.5g of vancomycin mixed with 2ml normal saline for each time. A total of 5gm of vancomycin® powder will be applied.

(2) Control group protocol. 2ml normal saline will be spread on sternal edge immediately after sternotomy and before sternal closure.

(3) Common perioperative protocols in both groups regarding sternal wound infection prevention: i. Intravenous antibiotics: Cefazolin 1g q8h for 24hrs. Patients with known cephalosporin or beta-lactam allergy will receive single dose Vancomycin plus Gentamicin.

ii. Skin preparation: Skin shave will be performed on the operation day after injection of prophylactic antibiotics. Skin sterilization will be prepared with alcohol beta-iodine scrub followed by Hibitane (Chlorhexidine Gluconate).

iii. Bone wax: bone wax usage for hemostasis will be minimized and be removed as much as possible before sternal closure.

iv. Internal mammary artery harvest: Bilateral internal mammary arteries will be used at the discretion of the individual surgeon.

v. Sternum closure: our standard is 8 single wires closure. Replacement of any 2 single wires with a figure of eight wire or addition of Robicsek technique will depend on the decision of the individual surgeon.

vi. Subcutaneous and skin closure: interrupted Vircyl for subcutaneous closure and staples for skin.

vii. Blood glucose control: intravenous insulin infusions to maintain serum blood glucose level between 120 and 180 mg/dL.

viii. Chest hugger: postoperative chest hugger will be used according to the preference of the individual patient.

ix. Delayed sternal closure: The decision is based on the discretion of the individual surgeon. If a delayed closure is attempted, the vancomycin paste will still be applied over the unclosed sternal edge.

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3. Outcome Measurement. Details described in other section.

(1) Sternal wound. Measurement on postoperative day (POD) 7, 30, and 90, based on the definition from Centers for Disease Control and Prevention.

(2) Serum Vancomycin levels. Sampling time: postoperative day 0, 3 and 7. Measurement: High performance liquid chromatography (HPLC).

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4. Data and Registry.

1. Data dictionary. Each variable in a row, presented in the following order: Variable, Coding Information, Range (numeric data) or Levels (categorial data), Information Source i. Sex, Sex, M or F, Electrical Medical Record

ii. Age, Age, 20 - 100, Electrical Medical Record

iii. Height, Body Height, 100 - 200, Electrical Medical Record

iv. Weight, Body Weight, 30 - 150, Electrical Medical Record

v. DM, Diabetes Mellitus, 1 or 0, Electrical Medical Record

vi. DM control, Diabetes Mellitus control method, 0 or Oral or Insulin, Electrical Medical Record

vii. HTN, Hypertension, 1 or 0, Electrical Medical Record

viii. PAOD, Peripheral artery occlusion disease, 1 or 0, Electrical Medical Record

ix. Dialysis, Dialysis, 0 or HD or PD, Electrical Medical Record

x. OldCVA, Old Cerebral Vascular Accident, 1 or 0, Electrical Medical Record

xi. CarotidStenosis, Carotid Artery Stenosis, 1 or 0, Electrical Medical Record

xii. LiverDisease, Liver Disease, 1 or 0, Electrical Medical Record

xiii. COPD, Chronic Obstructive Pulomnary Disease, 1 or 0, Electrical Medical Record

xiv. Alcohol, Alcohol consumption, 1 or 0, Electrical Medical Record

xv. Tobacco, Tobacco use, Never or Current or Former, Electrical Medical Record

xvi. NYHA, NYHA Functional Class, 1, 2, 3, 4, Electrical Medical Record

xvii. Afib, Atrial Fibrillation; paroxysmal, persistent, permanent, Electrical Medical Record

xviii. LVEF, Left Ventricular Ejection Fraction on preoperative echocardiography(%); 1 - 100, Electrical Medical Record

xix. Procedure, Surgical Procedure, "AVR MVR DVR CABG Aorta others", Electrical Medical Record

xx. XCtime, Cross-Clamp time (mins), 0 - 1000, Electrical Medical Record

xxi. CPBtime, Cardiopulmonary bypass time (mins), 0 - 1000, Electrical Medical Record

xxii. delay.closure, delayed sternal closure, 1 or 0, Electrical Medical Record

xxiii. SIMA, Single internal mammary artery graft, 1 or 0, Electrical Medical Record

xxiv. BIMA, Bilateral internal mammary artery graft, 1 or 0, Electrical Medical Record

xxv. BUN, Blood Urea Nitrogen , 0 - 100, Hospital Lab

xxvi. Cr, Serum Creatinine , 0 - 20, Hospital Lab

xxvii.AST, Serum Aspartate Aminotransferase level , 0 - 1000, Hospital Lab

xxviii.ALT, Serum Alanine Aminotransferase level , 0 - 1000, Hospital Lab

xxix. WBC, White blood cell count , 0 - 10, Hospital Lab

xxx. Albumin, serumAlbumin , 0 - 10, Hospital Lab

xxxi. Prealbumin, Serum Prealbumin level , 0 - 50, Hospital Lab

xxxii.TRF, Serum Transferrin level , 0 - 200, Hospital Lab

xxxiii.Serum Zinc level, Serum Zinc level , 70-120, Hospital Lab

xxxiv.TG, serum triglycerol level , 0 - 1000, Hospital Lab

xxxv.CHOL, serum CHOL level, 0 - 1000, Hospital Lab

xxxvi.HDL, serum high density lipoprotein level , 0 - 100, Hospital Lab

xxxvii.LDL, serum low density lipoprotein level , 0 - 1000, Hospital Lab

xxxviii.HbA1c, hemoglobin A1c level , 0 - 100, Hospital Lab

xxxix.Vanco.POD0, Serum Vancomycin level on postoperative day 0, 0 - 50, Study lab

xl. Vanco.POD3, Serum Vancomycin level on postoperative day 3, 0 - 50, Study lab

xli. Vanco.POD7, Serum Vancomycin level on postoperative day 7, 0 - 50, Study lab

xlii. Stroke, postoperative Stroke, 1 or 0, Electrical Medical Record

xliii. Bacteremia, postoperative Bacteremia, 0 or the bacteria species, Electrical Medical Record

xliv. Pneumonia, postoperative Pneumonia, 1 or 0, Electrical Medical Record

xlv. Resistance.pathogen, Resistance pathogen, 1 or 0, Electrical Medical Record

xlvi. Intubation.days, Intubation days, 0 - 100, Electrical Medical Record

xlvii. Mechanical.support.type, postoperative Mechanical support type, "IABP ECMO VAD", Electrical Medical Record

xlviii. Parenteral.nutrition, postoperative Parenteral nutrition, 0 or PPN or TPN, Electrical Medical Record

xlix. ICU.days, ICU days, 1-100, Electrical Medical Record

l. Hospital.days, Hospital days, 1-100, Electrical Medical Record

li. ThirtyDays.mortality, 30 days mortality, 1 or 0, Electrical Medical Record

lii. Hospital.mortality, Hospital mortality, 1 or 0, Electrical Medical Record

liii. Wound.POD7, Sternal wound condition on postoperative day 7, normal or superficial sternal infection or deep sternal infection, Study Nursing Practitioner

liv. Wound.POD30, Sternal wound condition on postoperative day 30, normal or superficial sternal infection or deep sternal infection, Study Nursing Practitioner

lv. Wound.POD90, Sternal wound condition on postoperative day 90, normal or superficial sternal infection or deep sternal infection, Study Nursing Practitioner

2. Data Collection:

i. Clinical parameters collection. All patient demographic data and parameters of STS (society of thoracic surgery) database will be recorded, such as age, sex, cause of cardiac diseases, co-morbidities, concurrent medication, body mass index, nutrition status, operation details, days of hospital and ICU stay, hospital cost, any surgical complications, and any documented infection and their microbiology susceptibility test results.

ii. Biochemistry and blood cell count. Data such as creatinine levels, fasting sugar levels, HbA1C, liver enzymes, nutrition status, and white cell count will be measured on postoperative day 0, 3, and 7 in the central laboratory of National Cheng Kung University Hospital.

3. Data management. i. Two registrars are responsible for data registry. Data are stored in spread sheet format.

ii. Missing Data. All data source are available in NCKUH's electrical medical record. Any variable with missing data more than 3% will be dropped.

4. Report for adverse events. The investigators are responsible for adverse events reporting to our institution review board (NCKUH IRB). The reporting procedure is generally based on the form from the Council for International Organizations of Medical Sciences (CIOMS). The definition and requirement for Serious Adverse Event (SAE), Suspected Unexpected Serious Adverse Reaction (SUSAR), and Unanticipated Problems (UP), are available at our IRB website: http://nckuhirb.med.ncku.edu.tw/upload/download/file20191217230754.pdf.

5. Quality assurance. This is a single institution study. Quality assurance will be done by monthly investigator meeting in our hospital, which including the following:

i. Data checks. Monthly data visualization report, either histogram for continuous variable or bar chart for categorial variable, will be used for detection of any data running out of range or having inconsistency with predefined rules.

ii. Source data verification. Accuracy and completeness of the registry data will be double checked by the primary care nursing practitioners by comparison with the electrical medical record system.

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5. Data Analysis, Statistical and Pharmacokinetic Analysis

1. For clinical data, continuous variables are expressed are as means ± standard deviation and analyzed with t-test. Categorical variables are presented as numbers (percentages) and Pearson's χ 2 or Fisher's exact test was used where appropriate. Univariate and multivariate logistic regression model and Cox model will be used to analyze risk factors for sternal wound infection. Significance was set at P < 0.05. Statistical analysis was performed using R, version 3.2.5 (R Foundation for Statistical Computing, Vienna, Austria.).

2. For serum Vancomycin levels, non-compartmental analysis will be conducted with WinNonlin software (www.certara.com). The population pharmacokinetics will be analyzed with nonlinear mixed-effects modeling using in NONMEN 7.3 (Icon Development Solutions, Hanover, MD). Xpose (xpose.sourceforge.net), PsN (psn.sourceforge.net), and R, version 3.2.5 (R Foundation for Statistical Computing, Vienna, Austria.) were used for postprocessing of NONMEM output.

Recruitment information / eligibility

• Status: Recruiting
• Enrollment: 360
• Est. completion date: December 31, 2024
• Est. primary completion date: December 31, 2024
• Accepts healthy volunteers: No
• Gender: All
• Age group: 20 Years and older

Eligibility

Inclusion Criteria:

• All adult patients admitted for elective cardiac surgery via first-time full sternotomy, such as coronary artery disease, valvular heart disease, adult congenital heart disease, aortic aneurysm.

Exclusion Criteria:

• redo sternotomy surgery

• minimal invasive or non-full-sternotomy cardiac surgery

• emergent surgery with preoperative shock or cardiopulmonary resuscitation

• heart transplant or ventricular assist device surgery

• patients who have any preoperative mechanical support including IABP, ECMO, or ventricular assist device

• evidence of Vancomycin allergy

• preexisting infection requiring preoperative regular antibiotics

• perioperative immunosuppressive requirement

• Patients with known cephalosporin or beta-lactam allergy who requires Vancomycin plus Gentamicin for intravenous prophylaxis will also be excluded because the intravenous Vancomycin will affect the further measurement of serum Vancomycin level after topical application

Related Conditions & MeSH terms

• Infections
• Sternal Wound Infection
• Wound Infection

Intervention

Drug:
• Vancomycin Hydrochloride
Vancomycin will be spread on sternal edge immediately after sternotomy and before sternal closure

Other:
• Normal Saline
2 ml normal saline will be spread on sternal edge immediately after sternotomy and before sternal closure

Locations

Country	Name	City	State
Taiwan	National Cheng Kung University Hospital	Tainan	???

Sponsors (1)

Lead Sponsor	Collaborator
National Cheng-Kung University Hospital

Country where clinical trial is conducted

Taiwan, 

References & Publications (6)

Hamman BL, Stout LY, Theologes TT, Sass DM, da Graca B, Filardo G. Relation between topical application of platelet-rich plasma and vancomycin and severe deep sternal wound infections after a first median sternotomy. Am J Cardiol. 2014 Apr 15;113(8):1415-9. doi: 10.1016/j.amjcard.2013.12.046. Epub 2014 Jan 31. — View Citation

Lander HL, Ejiofor JI, McGurk S, Tsuyoshi K, Shekar P, Body SC. Vancomycin Paste Does Not Reduce the Incidence of Deep Sternal Wound Infection After Cardiac Operations. Ann Thorac Surg. 2017 Feb;103(2):497-503. doi: 10.1016/j.athoracsur.2016.10.020. Epub 2016 Dec 24. — View Citation

Lazar HL, Barlam T, Cabral H. The effect of topical vancomycin applied to sternotomy incisions on postoperative serum vancomycin levels. J Card Surg. 2011 Sep;26(5):461-5. doi: 10.1111/j.1540-8191.2011.01300.x. — View Citation

Lazar HL, Ketchedjian A, Haime M, Karlson K, Cabral H. Topical vancomycin in combination with perioperative antibiotics and tight glycemic control helps to eliminate sternal wound infections. J Thorac Cardiovasc Surg. 2014 Sep;148(3):1035-8; 1038-40. doi: 10.1016/j.jtcvs.2014.06.045. Epub 2014 Jul 2. — View Citation

Lazar HL, Salm TV, Engelman R, Orgill D, Gordon S. Prevention and management of sternal wound infections. J Thorac Cardiovasc Surg. 2016 Oct;152(4):962-72. doi: 10.1016/j.jtcvs.2016.01.060. Epub 2016 Aug 8. No abstract available. — View Citation

Vander Salm TJ, Okike ON, Pasque MK, Pezzella AT, Lew R, Traina V, Mathieu R. Reduction of sternal infection by application of topical vancomycin. J Thorac Cardiovasc Surg. 1989 Oct;98(4):618-22. — View Citation

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Sternal wound infection condition on postoperative day 7	Sternal wound condition evaluate by study nursing practitioner, according to the definition of sternal wound infection based on the Centers for Disease Control and Prevention.; Depths 1 and 2 limited to the subcuticular and subcutaneous layers with no involvement of the sternum are de?ned as a super?cial sternal infection (SSI).; Depths 3 and 4, which involved the sternal bone or wires and collections beneath the sternum, are defined as deep sternal infection (DSI).; A wound was considered infected only if a positive culture was obtained.; The sternal wound condition will be coded as "non-infection", "SSI", "DSI"	postoperative day 7
Primary	Sternal wound infection condition on postoperative day 30	Sternal wound condition evaluate by study nursing practitioner, according to the definition of sternal wound infection based on the Centers for Disease Control and Prevention.; Depths 1 and 2 limited to the subcuticular and subcutaneous layers with no involvement of the sternum are de?ned as a super?cial sternal infection (SSI).; Depths 3 and 4, which involved the sternal bone or wires and collections beneath the sternum, are defined as deep sternal infection (DSI).; A wound was considered infected only if a positive culture was obtained.; The sternal wound condition will be coded as "non-infection", "SSI", "DSI"	postoperative day 30
Primary	Sternal wound infection condition on postoperative day 90	Sternal wound condition evaluate by study nursing practitioner, according to the definition of sternal wound infection based on the Centers for Disease Control and Prevention.; Depths 1 and 2 limited to the subcuticular and subcutaneous layers with no involvement of the sternum are de?ned as a super?cial sternal infection (SSI).; Depths 3 and 4, which involved the sternal bone or wires and collections beneath the sternum, are defined as deep sternal infection (DSI).; A wound was considered infected only if a positive culture was obtained.; The sternal wound condition will be coded as "non-infection", "SSI", "DSI"	postoperative day 90
Secondary	Serum Vancomycin exposure on postoperative day 0	Serum Vancomycin concentration (mcg/mL) measured with HPLC (high performance liquid chromatography)	postoperative day 0
Secondary	Serum Vancomycin exposure on postoperative day 3	Serum Vancomycin concentration (mcg/mL) measured with HPLC (high performance liquid chromatography)	postoperative day 3
Secondary	Serum Vancomycin exposure on postoperative day 7	Serum Vancomycin concentration (mcg/mL) measured with HPLC (high performance liquid chromatography)	postoperative day 7