Hematological Factors and Iron Status in Aerobic Versus Anaerobic Training in Athletic Females

Sports Anemia Clinical Trial

Official title:

Hematological Factors and Iron Status in Aerobic Versus Anaerobic Training in Athletic Females: An Observational Study

Clinical Trial Details — Status: Completed

Administrative data

• NCT number: NCT05707455
• Other study ID #: P.T.REC/012/004727
• Status: Completed
• Start date: September 1, 2022
• Est. completion date: October 31, 2022

Study information

• Verified date: September 2023
• Source: Cairo University
• Contact: n/a
• Is FDA regulated: No
• Study type: Observational

Clinical Trial Summary

This study aimed to evaluate the hematological factors and iron status in aerobic versus anaerobic training in athletic females.

Description:

In both the short and long term, physical training causes iron status deterioration in sporty females. Nonetheless, little is known about the effect of different forms of exercise (aerobic versus anaerobic) on haematological variables and iron status in sporty females during adolescence. As a result, the purpose of this study was to compare the haematological variables and iron status in aerobic versus anaerobic exercise in adolescent female athletes. Another goal was to compare aerobic and anaerobic groups in terms of iron status classifications, such as normal iron status, iron deficiency with or without anaemia.

Recruitment information / eligibility

• Status: Completed
• Enrollment: 25
• Est. completion date: October 31, 2022
• Est. primary completion date: October 31, 2022
• Gender: Female
• Age group: 16 Years to 19 Years

Eligibility

Inclusion Criteria:

• Healthy, virginal, nonsmoker, female athletes.
• Age from 16 to 19 years.
• Body mass index < 25 kg/m2.
• Having the same socio-economic level.
• Following a normal balanced nutrition.

Exclusion Criteria:

• Using any medical or hormonal therapy that might influence the iron status.
• Vegetarian athletes.
• Having menorrhagia or amenorrhea.
• Chronic inflammatory condition or existing infection.
• Diabetic, hypertensive or cardiac athletes.
• Haematological disease (with the exception of iron deficiency with or without anaemia).
• Having transfusion of blood.

Related Conditions & MeSH terms

• Sports Anemia

Intervention

Other:
• evaluating blood levels of hemoglobin, hematocrit, red blood cell count, mean corpuscular volume, mean corpuscular hemoglobin concentration, serum transferrin and serum ferritin.
evaluating blood levels of hemoglobin, hematocrit, red blood cell count, mean corpuscular volume, mean corpuscular hemoglobin concentration, serum transferrin and serum ferritin.

Locations

Country	Name	City	State
Egypt	Local clubs	Cairo

Sponsors (1)

Lead Sponsor	Collaborator
Cairo University

Country where clinical trial is conducted

Egypt, 

Outcome

Type	Measure	Description	Time frame	Safety issue
Primary	Assessment of hemoglobin (Hb)	Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure hemoglobin (Hb).	2 months
Primary	Assessment of hematocrit (Hct)	Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure hematocrit (Hct).	2 months
Primary	Assessment of red blood cell (RBC) count	Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure red blood cell (RBC) count.	2 months
Primary	Assessment of mean corpuscular volume (MCV)	Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure mean corpuscular volume (MCV).	2 months
Primary	Assessment of mean corpuscular hemoglobin concentration (MCHC)	Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. An automatic cell counter (Sysmex XS 1000, Japan) was used to measure mean corpuscular hemoglobin concentration (MCHC).	2 months
Primary	Assessment of serum transferrin	Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. The serum sample was centrifuged, and the serum transferrin was measured using COBAS Integra 400 plus, Switzerland.	2 months
Primary	Assessment of serum ferritin	Blood samples were collected between 8:00 and 9:00 a.m. after an overnight fast and time of relaxation. The athletic girls were asked to refrain from exercising the day before sample. Blood was taken from the antecubital vein and placed in one EDTA tube and one beads tube for serum separation. The serum sample was centrifuged, and the serum ferritin was measured using ADVIA Centaur XPT, Germany.	2 months
Secondary	Anthropometric measures	A weight-height scale was used to assess the weight (Kg) and height (m) for each female athlete in the two groups. The body mass index (BMI) was then calculated by dividing the weight by the height squared (Kg/m2).	2 months
Secondary	Assessment of participation age to sport	Each female athlete was asked about her age of participation to sport to assess her training profile.	2 months
Secondary	Assessment of number of hours of training per week	Each female athlete was asked about the number of hours of training per week to assess her training profile.	2 months
Secondary	Assessment of training experience	The training experience in years was calculated by subtracting the female athlete's age of participation to training from her chronological age.	2 months